165,173 results

1. Sp_PE

   Plasmid

   #212038

   Purpose

   Bacterial expression of the PE2 proteins for genome editing in E. coli

   Depositor

   Quanjiang Ji

   Article

   Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

   Insert

   PE2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   L-arabinose-inducible promoter PBAD

   Available since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-dCas9-p300 Core

   Plasmid

   #61357

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   CMV

   Available since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCold TDP43 WT

   Plasmid

   #27451

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   Factor Xa, GST, and His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET His6 GFP TEV LIC cloning vector (1GFP)

   Plasmid

   #29663

   Depositor

   Scott Gradia

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   6xHis, GFP, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPRia-v2

   Plasmid

   #84832

   Purpose

   CRISPRi/a V2 library parental plasmid

   Depositor

   Jonathan Weissman

   Article

   Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

   Inserts

   sgRNA
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-ULK2

   Plasmid

   #97075

   Purpose

   Expresses ULK2 in mammalian cells

   Depositor

   Pasquale Vito

   Article

   Scudiero et al Cell Death Dis. 2017 Feb 23;8(2):e2627.

   Insert

   ULK2 (ULK2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti CMV TRE3G Neo GFP-Lamin A wildtype

   Plasmid

   #118709

   Purpose

   Lentiviral TET-ON inducible GFP-Lamin A wildtype

   Depositor

   Tom Misteli

   Article

   Kubben et al Methods. 2015 Sep 1. pii: S1046-2023(15)

   Insert

   GFP-Lamin A human wildtype (LMNA Human)
   

   Use

   Lentiviral; Tet-on inducible

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV TRE3G (TET-ON)

   Available since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. CAG-BFP-sesRNA-GFP-WPRE-pA

   Plasmid

   #192063

   Purpose

   Expression of BFP, sesRNA in mammalian cells, with GFP as efRNA.

   Depositor

   Josh Huang

   Article

   Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

   Insert

   sesRNA for tdTom
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3b-8XGliBS:EGFP

   Plasmid

   #84602

   Purpose

   Gli protein-dependent EGFP reporter for analysis of Hedgehog signaling

   Depositor

   James Chen

   Article

   Hyman et al Proc Natl Acad Sci U S A. 2009 Aug 18;10

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   8X Gli-binding site

   Available since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-JEDI-2P-WPRE (AAV9)

    Viral Prep

    #179464-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hSyn-JEDI-2P-WPRE (#179464). In addition to the viral particles, you will also receive purified pAAV-hSyn-JEDI-2P-WPRE plasmid DNA. hSyn-driven expression of the genetically encoded voltage indicator JEDI-2P. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Francois St-Pierre

    Article

    Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

    Promoter

    Syn

    Tags

    None

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

11. pClneoMyc rat S-SCAM alpha

    Plasmid

    #40213

    Depositor

    Yoshimi Takai, Yutaka Hata

    Article

    Hirao et al J Biol Chem. 1998 Aug 14;273(33):21105-1

    Insert

    S-SCAM (Magi2 Rat)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pXPR_206

    Plasmid

    #96920

    Purpose

    for SaCas9 knockout, lentiviral expression of S. aureus Cas9 (SauCas9) and gRNA scaffold

    Depositor

    David Root, John Doench

    Article

    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-nEF-ChRmine-mScarlet (AAV8)

    Viral Prep

    #137158-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-nEF-ChRmine-mScarlet (#137158). In addition to the viral particles, you will also receive purified pAAV-nEF-ChRmine-mScarlet plasmid DNA. nEF-driven expression of ChRmine-mScarlet. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    nEF

    Tags

    mScarlet

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

14. MelanoTag-Control-PURO (pLJC5-GPR143-mScarlet-1xMyc)

    Plasmid

    #160368

    Purpose

    Stable expression of GPR143-mScarlet-1xMyc fusion protein for use as a control with MelanoTag-PURO

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Insert

    GPR143 (GPR143 Human)
    

    Use

    Lentiviral

    Tags

    mScarlet-myc

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFRT-TODestGFP_RNAseH1

    Plasmid

    #65784

    Purpose

    expresses GFP-tagged RNAseH1 in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    RNaseH1 (RNASEH1 Zebrafish, Human)
    

    Use

    Tags

    GFP and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGLO-GFP-1UAG

    Plasmid

    #82500

    Purpose

    Expresses GFP with 1 UAG codon at the amino acid position 3

    Depositor

    Chang Liu

    Article

    Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

    Insert

    Green Fluorescent Protein with 1 UAG codon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Added an UAG codon at the amino acid position 3

    Promoter

    AraBAD

    Available since

    Sept. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. MT363

    Plasmid

    #65858

    Purpose

    The gRNA construct is designed for constitutive expression of salicylate hydroxylase for degradation of salicylic acid to catechol.

    Depositor

    Detlef Weigel

    Article

    Todesco et al Nature. 2010 Jun 3;465(7298):632-6. doi:

    Insert

    nahG
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. KA1775_pPBCAG-rtTAM2-IH

    Plasmid

    #124167

    Purpose

    piggyBac-based expression of rtTA-M2

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    rtTAM2-IRES-Hph
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. AbVec1.1-IGLC2-XhoI

    Plasmid

    #99575

    Purpose

    Expression of immunoglobulin light chains in mammalian cells, human lambda 2 constant region

    Depositor

    Hedda Wardemann

    Article

    Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W

    Plasmid

    #67977

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2AmCherry cassette, WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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