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Venus-GalT

Plasmid

#11931

Depositor

Jennifer Lippincott-Schwartz

Article

Cole et al Science. 1996 Aug 9. 273(5276):797-801.

Insert

GalT (GALT Human)

Expression

Mammalian

Mutation

Amino acids 1-60 of the human galactosyltransfera…

Available Since

Sept. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pCDH-cGAS-E225A D227A-mCherry2-EF1-Puro

Plasmid

#132771

Purpose

Interacts with cytoplasmic DNA. Useful for identifying sites of nuclear envelope rupture.

Depositor

Jan Lammerding

Article

Denais et al Science. 2016 Apr 15;352(6283):353-8. do

Insert

Cyclic GMP-AMP synthase (CGAS Human)

Use

Lentiviral

Tags

mCherry2

Expression

Mammalian

Mutation

E225A D227A

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pHAHA

Plasmid

#16586

Depositor

Bert Vogelstein

Article

Yu et al Mol Cell. 2001 Mar . 7(3):673-82.

Type

Empty backbone

Tags

2x HA

Expression

Mammalian

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-NORAD

Plasmid

#120383

Purpose

Expression vector for lncRNA NORAD

Depositor

Igor Ulitsky

Article

Tichon et al Nat Commun. 2016 Jul 13;7:12209. doi: 10

Insert

NORAD (NORAD Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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EFIa-iTGB1

Plasmid

#115799

Purpose

Integrin B1 overexpression

Depositor

Joan Massague

Article

Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

Insert

iTGB1 (ITGB1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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BiP-mGFP

Plasmid

#62231

Purpose

Encodes hamster BiP/GRP78/HSPA5 fused to monomeric EGFP. Useful for studying BiP mobility and trafficking in live cells.

Depositor

Erik Snapp

Article

Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2

Insert

hamster BiP-mGFP-KDEL

Tags

mGFP

Expression

Mammalian

Promoter

cmv

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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GI-Gal4 DBD

Plasmid

#42500

Purpose

GIGANTEA fused with Gal4 DNA binding domain (DBD), part of a light-inducible transcription factor

Depositor

Ricardo Dolmetsch

Article

Yazawa et al Nat Biotechnol. 2009 Oct;27(10):941-5. d

Insert

GIGANTEA fused with Gal4 DBD

Tags

Gal4 DNA binding domain (DBD)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mem-TurboID

Plasmid

#149409

Purpose

Mammalian expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in mammalian expression plasmid. Amp/Neo selection

Depositor

Jonathan Long

Article

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Insert

mem-TurboID

Tags

HA and V5

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pPS

Plasmid

#85801

Purpose

Backbone for pooled cloning of Perturb-seq libraries

Depositor

Aviv Regev

Article

Dixit et al Cell. 2016 Dec 15;167(7):1853-1866.e17.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

March 14, 2017

Availability

Academic Institutions and Nonprofits only

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pAD48-daf-2 RNAi

Plasmid

#34834

Depositor

Cynthia Kenyon

Article

Dillin et al Science. 2002 Oct 25;298(5594):830-4.

Insert

daf-2 (daf-2 Nematode)

Use

RNAi

Expression

Bacterial

Available Since

June 11, 2012

Availability

Academic Institutions and Nonprofits only
pLUT7 HA-GLI1

Plasmid

#62970

Purpose

Expresses HA-tagged Gli1

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

GLI1 (GLI1 Human)

Use

Lentiviral

Tags

HA

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5myc RhoA L63

Plasmid

#15900

Depositor

Alan Hall

Article

Nobes et al J Cell Biol. 1999 Mar 22. 144(6):1235-44

Insert

RhoA (RHOA Human)

Tags

myc

Expression

Mammalian

Mutation

Q63L

Available Since

Nov. 15, 2007

Availability

Academic Institutions and Nonprofits only
pEGFP-C3-Exo84

Plasmid

#53762

Purpose

pEGFP-C3 plasmid that expresses Exo84

Depositor

Channing Der

Article

Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

Insert

Exo84 (EXOC8 Human)

Tags

GFP

Expression

Mammalian

Available Since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only

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pGL4 mIL-17 2kb promoter + CNS-5

Plasmid

#20128

Depositor

Warren Strober

Article

Fuping et al 2008 Nov;9(11):1297-306. Epub 2008 Oct 1

Insert

mIL-17 (Il17a Mouse)

Use

Luciferase

Available Since

Jan. 16, 2009

Availability

Academic Institutions and Nonprofits only

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Cdk6-DN-HA

Plasmid

#1869

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk6 (CDK6 Human)

Tags

HA

Expression

Mammalian

Mutation

Dominant negative, D to N in conserved KLAD*FGLAR…

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only
pTRE-Tight caspase-3 (p12)::nz [TU#817]

Plasmid

#16084

Depositor

Martin Chalfie

Article

Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 1

Insert

caspase-3 (p12) :: nz (CASP3 Human)

Expression

Mammalian

Available Since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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3492 pcDNA3 Bad

Plasmid

#8778

Depositor

Stanley Korsmeyer

Article

Harada et al Proc Natl Acad Sci U S A 2001 Aug 14;98(

Insert

Bad (Bad Mouse)

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
k3(long) luciferase

Plasmid

#11110

Depositor

Anjana Rao

Article

Tsai et al Mol Cell Biol. 1996 Feb . 16(2):459-67.

Insert

k3(L) (TNF Human)

Tags

luciferase

Expression

Bacterial

Available Since

April 24, 2006

Availability

Academic Institutions and Nonprofits only
pENTR_L1-YFP-Turbo-L2

Plasmid

#127350

Purpose

gateway entry vector for making N-terminal YFP-TurboID fusions with nuclear and non-nuclear proteins

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

TurboID (BirA mutant)

Use

Gateway Cloning

Tags

GS linker, V5, and YFP

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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ARHGEF11(DHPH)-CRY2-mCherry

Plasmid

#89481

Purpose

Also known as optoGEF-RhoA, encodes for a fusion between CRY2(1-498)-mCherry and the DHPH domain of ARHGEF11

Depositor

Xavier Trepat Guixer

Article

Valon et al Nat Commun. 2017 Feb 10;8:14396. doi: 10

Insert

ARHGEF11(DHPH) - CRY2(1-498) - mCherry (CRY2, ARHGEF11 Mustard Weed, Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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