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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 81,667 results for: cloning

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Showing: 3521 - 3540 of 81667 results

  1. pCJ136

    Plasmid
    #162665
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162665-plasmid-map-sequence-id-321451

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  5. YE1-BE4max-NG

    Plasmid
    #138159
    Purpose
    C-to-T base editor
    Depositor
    David Liu
    Publication
    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rAPOBEC1(YE1)-nSpCas9(NG)-UGI-UGI
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Within rAPOBEC1 W90Y + R126E; within Cas9 D10A + …
    Promoter
    CMV
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    138159-plasmid-map-sequence-id-272341

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  6. pT3-EF1A-MYC-IRES-lucOS

    Plasmid
    #129776
    Purpose
    Transposon based vector that expresses human MYC followed by IRES and firefly luciferase and the antigens SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), and OVA323-339
    Depositor
    Amaia Lujambio
    Publication
    Ruiz de Galarreta et al Cancer Discov. 2019 Jun 11. pii: 2159-82
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human MYC (MYC Human)
    Tags
    OS: SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), a…
    Expression
    Mammalian
    Promoter
    EF1A
    Available since
    Feb. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    129776-plasmid-map-sequence-id-263368

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  7. PB_tre_dCas9_VP160

    Plasmid
    #126031
    Purpose
    PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion
    Depositor
    Mauro Calabrese
    Publication
    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9 fusion with VP160
    Hygromycin resistance
    Use
    CRISPR; Piggybac
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically inactive Cas9)
    Promoter
    EF1 alpha and TRE
    Available since
    June 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    126031-plasmid-map-sequence-id-246110

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  9. pCLX-PGK-hTERT

    Plasmid
    #114315
    Purpose
    Constitutive expression of the hTert protein
    Depositor
    Patrick Salmon
    Insert
    hTERT (TERT Human)
    Use
    Lentiviral
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114315-plasmid-map-sequence-id-218852

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  10. MoClo Plant Parts II and Infrastructure Kit

    Plasmid kit
    #1000000135
    Purpose
    Modules connect MoClo and Gateway for hierarchical assembly of plant multigene constructs; contains yeast two-hybrid and bacterium-to-plant translocation vectors. Partially compatible with GoldenBraid.
    Depositor
    Johannes Stuttmann
    Application
    Cloning and Synthetic Biology
    Vector type
    Plant Expression
    Cloning type
    Golden Gate (MoClo)
    Available since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only

  15. TCRalpha_E2A_SP-SNAPf-TCRbeta_P2A_CD3e_T2A_CD3zeta

    Plasmid
    #89347
    Purpose
    2nd generation lentiviral vector which expresses SNAP tagged TCRbeta and other TCR subunits (Jurkat alleles) separated via 2A sequences
    Depositor
    Ron Vale
    Publication
    Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do
    Insert
    Jurkat TCRalpha/beta alleles and CD3e and CD3z
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    SFFV
    Available since
    May 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89347-plasmid-map-sequence-id-176322

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  16. TCRalpha_E2A_SP-SNAPf-TCRbeta_P2A_CD3e_T2A_CD3zeta

    Plasmid
    #89347
    Purpose
    2nd generation lentiviral vector which expresses SNAP tagged TCRbeta and other TCR subunits (Jurkat alleles) separated via 2A sequences
    Depositor
    Ron Vale
    Publication
    Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do
    Insert
    Jurkat TCRalpha/beta alleles and CD3e and CD3z
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    SFFV
    Available since
    May 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89347-plasmid-map-sequence-id-176322

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  17. pCMV-DeAct-GS1

    Plasmid
    #89445
    Purpose
    Expresses EGFP-GS1 (Human Gelsolin 53-176) in mammalian cells
    Depositor
    Brad Zuchero
    Publication
    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DeAct-GS1 (GSN Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Truncation spanning cytoplasmic Gsn amino acids 5…
    Promoter
    CMV
    Available since
    April 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89445-plasmid-map-sequence-id-177128

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  18. pCMV-DeAct-GS1

    Plasmid
    #89445
    Purpose
    Expresses EGFP-GS1 (Human Gelsolin 53-176) in mammalian cells
    Depositor
    Brad Zuchero
    Publication
    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DeAct-GS1 (GSN Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Truncation spanning cytoplasmic Gsn amino acids 5…
    Promoter
    CMV
    Available since
    April 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89445-plasmid-map-sequence-id-177128

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Showing: 3521 - 3540 of 81667 results