We narrowed to 62,257 results for: cloning
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Plasmid#102249PurposeExpresses fusion of CRY2PHR with mCherry and LAMP1 (1-415)DepositorAvailable SinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only
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G9A[SET]-dCas9
Plasmid#100089PurposeCatalytic domain [SET] of human G9A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertG9A (EHMT2 Human)
UseCRISPRTags3XFLag-NLS-G9A[SET]-dCas9-NLSExpressionMammalianMutationG9A[SET] includes aa 829-1209PromoterCMVAvailable SinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_ATG16L1_WT
Plasmid#82255PurposeGateway Donor vector containing ATG16L1 , part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-1BPNLS-Cas9-1BPNLS
Plasmid#87108PurposeBPNLS-Cas9-BPNLS expression plasmidDepositorInsert1BPNLS-Cas9-1BPNLS
UseCRISPRTags3xFLAGExpressionMammalianPromoterCAGAvailable SinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBad-HisB-RRvT
Plasmid#87364PurposeExpresses RRvT, a tandem heterodimer red fluorescent proteinDepositorInsertRRvT
TagsHis-TagExpressionBacterialPromoterpBad (Arabinose)Available SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti Lifeact-mRuby2 BlastR
Plasmid#84384PurposeLentiviral expression of mRuby2-tagged Lifeact with Blasticidin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsmRuby2PromoterCMV immediate earlyAvailable SinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-VPR
Plasmid#78898PurposeExpresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.DepositorInsertdCas9-VPR
UseCRISPRExpressionInsectPromoterpActin (Drosophila)Available SinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas9-polyA
Plasmid#72602Purposeexpress hCas9 byT7 promoter with polyadenine tailsDepositorInsertCas9-polyadenine
Tags81-bp polyadenylationPromoterT7Available SinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV_NLS-SaCas9-NLS-VPR
Plasmid#68496PurposeAAV vector containing SaCas9 fused to VPRDepositorInsertSaCas9-VPR
UseAAV and CRISPRTagsVPRExpressionMammalianPromoterCMVAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMU-PMr
Plasmid#61180PurposeFluorescent reporter for plasma membrane AtPIP2a-mCherry expressed under AtUBQ10 promoterDepositorInsertAtPIP2a
TagsmCherryExpressionPlantPromoterAtUBQ10Available SinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV-IFP2.0 T2A HO1
Plasmid#59427PurposeExpress infrared fluorescent protein IFP2.0 plus HO1 enzymeDepositorInsertIFP2.0T2AHO1 IRES EGFP
UseLentiviralPromoterCMVAvailable SinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR-PKAKTRClover
Plasmid#59146PurposeORF encoding PKA KTR mClover flanked by gateway sequencesDepositorInsertPKA Kinase Translocation Reporter (PRKACA Mouse, Human)
Available SinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
PUF Assembly Kit
Plasmid Kit#1000000051PurposeAssembly of Pumilio and FBF (PUF) homology domain based RNA-binding proteins for recognition of RNA targets.DepositorApplicationCloning and Synthetic Biology, Genome EditingVector TypeBacterial Expression, Mammalian ExpressionEditing TypePUFCloning TypeGolden GateAvailable SinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAC149-pCR8-dCas9VP160
Plasmid#48221PurposedCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expressionDepositorInsertdCas9(D10A;H840A) fusion with VP160 activation domain
UseCRISPR and Gateway CloningTagsHA Tag and VP160MutationD10A;H840A nuclease-deficientAvailable SinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAC91-pmax-dCas9VP64
Plasmid#48223PurposedCas9VP64 on pmax expression vectorDepositorInsertdCas9(D10A;H840A) fusion with VP64 activation domain
UseCRISPRTagsHA Tag and VP64ExpressionMammalianMutationD10A;H840A (catalytically inactive)PromoterCAGGSAvailable SinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSAM
Plasmid#45174DepositorTypeEmpty backboneTags6xHis and 6xHis/Thrombin/T7ExpressionBacterialPromoterT7Available SinceJune 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
gRNA_DNMT3a-T1
Plasmid#41821PurposeExpresses a guide RNA (gRNA) to target DNMT3a (T1 target sequence) for genome engineeringDepositorAvailable SinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti6-BMPR1A(Q233D)-V5
Plasmid#35638DepositorInsertBMP Receptor Type1A Q233D (BMPR1A Human)
UseLentiviralTagsV5ExpressionMammalianMutationGlutamine 233 to AspartatePromoterCMVAvailable SinceApril 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMXs-MYT1L
Plasmid#32926PurposeRetroviral expression of human Myt1LDepositorAvailable SinceNov. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Ngn2
Plasmid#32928PurposeRetroviral expression of mouse Ngn2DepositorAvailable SinceNov. 30, 2011AvailabilityAcademic Institutions and Nonprofits only