We narrowed to 54,151 results for: tra

1. pB-Halo,IRES-eGFP

   Plasmid

   #164519

   Purpose

   All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible GFP and constitutive rtTA and neomycin resistance)

   Depositor

   Mitchell Guttman

   Article

   Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF0917

   Plasmid

   #142198

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NCOA3 (NCOA3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHDM-SARS-CoV-2 Δ18

   Plasmid

   #164434

   Purpose

   Encodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Insert

   SARS-CoV-2 Spike truncated to remove 18 amino acids (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. GC52 pEGFPN1 anti-Gephyrin nanobody

   Plasmid

   #135224

   Purpose

   Mammalian expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an intrabody

   Depositor

   James Trimmer

   Article

   Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

   Insert

   anti-Gephyrin (Human) recombinant llama nanobody GC52 (GPHN L. glama (llama))
   

   Use

   Tags

   6xHis-HA-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEXP5-aHL_spytag_his

   Plasmid

   #107334

   Purpose

   Monomeric SpyTag-6×-His alpha-hemolysin

   Depositor

   George Church

   Article

   Stranges et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

   Insert

   alpha-hemolysin-SpyTag-6xHis
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

   Plasmid

   #171090

   Purpose

   CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.

   Depositor

   Sebastian Lourido

   Article

   Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

   Inserts

   Sth1 dCas9
   Chloramphenicol acetyltransferase
   TagBFP
   

   Use

   CRISPR; Toxoplasma gondii expression

   Tags

   3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…

   Expression

   Mutation

   D9A, H599A

   Promoter

   TUB1 (Toxoplasma gondii), transcriptionally linke…

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

   Plasmid

   #176534

   Purpose

   Bacterial expression of His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   CRISPR-associated IscB (Delaware Bay metagenome)
   

   Use

   Tags

   14xHis-MBP-bdSUMO and TwinStrep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKIF1C-GFP

   Plasmid

   #130977

   Purpose

   Expression of KIF1C-GFP in mammalian cells.

   Depositor

   Anne Straube

   Article

   Efimova et al J Cell Sci. 2014 Dec 15;127(Pt 24):5179-

   Insert

   KIF1C-GFP (KIF1C Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   5 silent mutations that make this construct RNAi …

   Promoter

   CMV

   Available since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. SS80 pEGFPN1 anti-SAPAP2 nanobody

   Plasmid

   #135228

   Purpose

   Mammalian expression of anti-SAPAP (Rat) recombinant llama nanobody for use as an intrabody

   Depositor

   James Trimmer

   Article

   Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

   Insert

   anti-SAPAP2 (Rat) recombinant llama nanobody SS80 (Dlgap2 L. glama (llama))
   

   Use

   Tags

   6xHis-HA-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Flag-Bcl2-Cb5

    Plasmid

    #18004

    Depositor

    Clark Distelhorst

    Article

    Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

    Insert

    ER targeted Bcl-2 (BCL2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C terminal of Bcl-2 replaced with Cytochrome b5. …

    Promoter

    CMV

    Available since

    June 4, 2008

    Availability

    Academic Institutions and Nonprofits only

11. Flag-Bcl2-Maob

    Plasmid

    #18005

    Depositor

    Clark Distelhorst

    Article

    Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

    Insert

    Mitochondria-targeted Bcl-2 (BCL2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C terminal of Bcl-2 replaced with Maob. Targets B…

    Promoter

    CMV

    Available since

    June 4, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pCI-Puro_mouse_Adar2_Tv1

    Plasmid

    #158111

    Purpose

    expresses mADAR2 shortest transcript, puromycin selection

    Depositor

    Emanuela Felley-Bosco

    Article

    Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

    Insert

    adar2 (Adarb1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSB63 - pL0_fLUC-I (CDS1)

    Plasmid

    #123180

    Purpose

    Golden Gate (MoClo; CDS1) compatible luciferase gene from Photinuspyralis with an intron for transient and stable expression in plants

    Depositor

    Carolyn Lee-Parsons, Erin Cram

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    Luciferase gene from Photinuspyralis with intron from U5 small nuclear ribonucleoprotein component (Arabidopsis thaliana)
    

    Use

    Luciferase; Part for plant expression

    Tags

    Expression

    Plant

    Mutation

    No BpiI and BsaI sites

    Promoter

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSB167 - pL2_pSB90_2x35S::fLUC-I::tNOS_tMAS::rLUC-I::pMAS

    Plasmid

    #123200

    Purpose

    binary plant vector for transient expression of a Renilla luciferase (rLUC, with intron) and a firefly luciferase (fLUC, with intron) in plants

    Depositor

    Carolyn Lee-Parsons, Erin Cram

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    2x35S::fLUC-I::tNOS tMAS::rLUC-I::pMAS
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHS0535 CRISPR-associated IscB (Chesapeake Bay) in pBR322 with Fn spacer

    Plasmid

    #176589

    Purpose

    Endogenous locus of CRISPR-associated IscB (Chesapeake Bay) with Fn spacer

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    CRISPR-associate IscB (Chesapeake Bay) locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Truncated CRISPR array to two direct repeats, rep…

    Promoter

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-Flt3-Y591F/Y919F

    Plasmid

    #74497

    Purpose

    Overexpression of FLT3 Y591F/Y919F mutant in mammalian cells

    Depositor

    Lars Rönnstrand

    Article

    Kazi et al J Biol Chem. 2012 Oct 19;287(43):36509-1

    Insert

    FLT3 (FLT3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y591F/Y919F, T227M

    Promoter

    CMV

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pTwist-SARS-CoV-2 Δ18 K417N+E484K+N501Y

    Plasmid

    #169469

    Purpose

    Encodes SARS-CoV-2 K417N+E484K+N501Y Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 K417N+E484K+N501Y Spike truncated to remove 18 amino acids (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. phBG-P53DD

    Plasmid

    #149707

    Purpose

    In vitro transcription of dominant negative form of P53 mRNA for RNA transfection into mammalian cells

    Depositor

    Erika Sasaki

    Article

    Watanabe et al Genes Cells. 2019 Jul;24(7):473-484. doi

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    Rna transcription

    Tags

    Expression

    Mutation

    deleted amino acid 14-301

    Promoter

    T7

    Available since

    June 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMSCV-Flt3-Y591F/Y919F

    Plasmid

    #74498

    Purpose

    Retroviral expression of FLT3 Y591F/Y919F

    Depositor

    Lars Rönnstrand

    Article

    Kazi et al J Biol Chem. 2012 Oct 19;287(43):36509-1

    Insert

    FLT3 (FLT3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Y591F/Y919F, T227M

    Promoter

    unknown

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pTwist-SARS-CoV-2 Δ18 B.1.351v2/wtRBD

    Plasmid

    #169467

    Purpose

    Encodes SARS-CoV-2 B.1.351v2/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 B.1.351v2/wtRBD Spike truncated to remove 18 amino acids (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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