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pWZL hygro H-Ras V12

Plasmid

#18749

Depositor

Scott Lowe

Publication

Serrano et al Cell. 1997 Mar 7. 88(5):593-602.

Insert

H-Ras V12 (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

V12

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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CD3 delta YFP

Plasmid

#11951

Depositor

Nico Dantuma

Publication

Menendez-Benito et al Hum Mol Genet. 2005 Oct 1. 14(19):2787-9

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CD3delta-YFP

Expression

Mammalian

Available since

June 22, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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mini-pHelper-1.0

Plasmid

#230933

Purpose

The mini version (8.4 kb) of Ad helper plasmid to provide E2A, E4orf6, and VA RNA for AAV production

Depositor

Qizhao Wang

Publication

Yang et al Mol Ther Nucleic Acids. 2025 May 14;36(2

Type

Empty backbone

Use

AAV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pSOUP

Plasmid

#165419

Purpose

The pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in Agrobacterium

Depositor

Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

Publication

Hayta et al Curr Protoc. 2021 Mar;1(3):e58. doi: 10.

Insert

Rep A

Use

Helper plasmid required for replication for all p…

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-hSyn-OsTIR1(F74G)

Plasmid

#140730

Purpose

OsTIR1(F74G)-P2A-mAID-EGFP-NES

Depositor

Masato Kanemaki

Publication

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(F74G)-P2A-mAID-EGFP-NES

Use

AAV

Expression

Mammalian

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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AAV.CBA.eGFP.2A.P301L-Tau

Plasmid

#140425

Purpose

AAV.CBA.eGFP.2A.P301L-Tau propagation reporter P301L-2N4R-hTau

Depositor

Bradley Hyman

Publication

Wegmann et al Sci Adv. 2019 Jun 26;5(6):eaaw6404. doi:

Insert

eGFP-2A-Tau(P301L) (MAPT Human, Synthetic)

Use

AAV

Tags

eGFP

Mutation

changed Proline 301 to Leucine

Promoter

CBA

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3.1-SARS-Spike

Plasmid

#145031

Purpose

Express SARS-CoV spike protein with C9 tag at C-terminal in mammalian cells

Depositor

Fang Li

Publication

Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SARS-CoV Spike (S Synthetic)

Tags

C9 and CD5 signal peptide

Expression

Mammalian

Promoter

CMV

Available since

April 16, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

13 Articles

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ER-mNeonGreen

Plasmid

#137804

Purpose

Visualization of the Endoplasmic Reticulum

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

KDEL

Expression

Mammalian

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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PLKO.1-Scrambled

Plasmid

#136035

Purpose

Scrambled shRNA (negative control) inserted into the PLKO.1 plasmid (CCTAAGGTTAAGTCGCCCTCG)

Depositor

Anthony Leung

Publication

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

None (Scrambled)

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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pAG153 FKBP-CFAST11

Plasmid

#130813

Purpose

Expresses FKBP-CFAST11 in mammalian cells

Depositor

Arnaud Gautier

Publication

Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FKBP-CFAST11

Tags

Myc-tag

Expression

Mammalian

Promoter

CMV

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)

Plasmid

#128604

Purpose

Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.

Depositor

Marvin Tanenbaum

Publication

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Inserts

24xMoonTag
kinesin family member 18b (KIF18B Human)

Expression

Mammalian

Promoter

Pcmv

Available since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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4xmts-mNeonGreen

Plasmid

#98876

Purpose

In vivo visualization of mitochondria (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

4xmts

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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AAV CMV-dSaCas9-KRAB-bGHpA

Plasmid

#106219

Purpose

AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

Depositor

Charles Gersbach

Publication

Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

Insert

dead S. aureus Cas9 KRAB

Use

AAV and CRISPR

Tags

HA-NLS

Expression

Mammalian

Mutation

D10A and N580A

Promoter

CMV

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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TurboID-V5_pRS415

Plasmid

#107167

Purpose

expresses V5-tagged TurboID in the yeast cytosol

Depositor

Alice Ting

Publication

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID (BirA mutant)

Expression

Yeast

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Available since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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S1030

Bacterial strain

#105063

Purpose

host strain for phage assisted continuous evolution

Depositor

David Liu

Publication

Carlson et al Nat Chem Biol. 2014 Mar;10(3):216-22. do

Bacterial resistance

Streptomycin and Tetracycline

Available since

March 21, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
TRE-KRAB-dCas9-IRES-GFP

Plasmid

#85556

Purpose

2nd generation lentiviral vector expressing a KRAB-dCas9 fusion protein and GFP

Depositor

Eric Lander

Publication

Fulco et al Science. 2016 Sep 29. pii: aag2445.

Insert

KRAB-dCas9-IRES-GFP

Use

CRISPR and Lentiviral

Tags

2x NLS, HA Tag, and KRAB domain

Expression

Mammalian

Promoter

TRE3G

Available since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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AAV-U6-sgRNA-hSyn-mCherry

Plasmid

#87916

Purpose

sgRNA expressing AAV construct with a mCherry reporter driven by hSyn promoter. (replaced the GFP in pX552 from Zhang lab with mCherry)

Depositor

Alex Hewitt

Publication

Hung et al Invest Ophthalmol Vis Sci. 2016 Jun 1;57

Insert

mCherry

Use

AAV and CRISPR

Expression

Mammalian

Promoter

hSyn

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pHR-PP7-2xmCherry-CAAX

Plasmid

#74925

Purpose

Expresses PP7-2xmCherry-CAAX

Depositor

Marvin Tanenbaum

Publication

Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

Insert

PP7-2xmCherry-CAAX

Use

Lentiviral

Expression

Mammalian

Available since

April 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA-Flag-Tet3

Plasmid

#60940

Purpose

Expresses Tet3 in mammalian cells

Depositor

Yi Zhang

Publication

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Ten-Eleven Translocation 3

Tags

flag

Expression

Mammalian

Promoter

CMV

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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mouse E-cadherin GFP (423)

Plasmid

#67937

Purpose

Mammalian expression of E-cadherin-GFP

Depositor

Alpha Yap

Publication

Truffi et al J Cell Sci. 2014 Jun 1;127(Pt 11):2420-3

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

E-cadherin (Cdh1 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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