163,297 results

1. pHR-UCOE-SFFV-Zim3-dCas9-BFP

   Plasmid

   #188776

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9-mTagBFP2
   

   Use

   Lentiviral

   Tags

   HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CW3SL-EGFP

   Plasmid

   #61463

   Purpose

   AAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes)

   Depositor

   Bong-Kiun Kaang

   Article

   Choi et al Mol Brain. 2014 Mar 11;7:17. doi: 10.118

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CaMKIIa

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCasPA

   Plasmid

   #113347

   Purpose

   Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa

   Depositor

   Quanjiang Ji

   Article

   Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5-HA-YFP-rictor

   Plasmid

   #73387

   Purpose

   Expresses HA and YFP tagged Rictor

   Depositor

   Jie Chen, Taekjip Ha

   Article

   Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

   Insert

   RPTOR Independent Companion Of MTOR, Complex 2 (RICTOR Human)
   

   Use

   Tags

   HA and YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pINDUCER20-α-Syn

   Plasmid

   #92200

   Purpose

   Retroviral overexpression vector (doxycycline-inducible) for α-Syn

   Depositor

   Huda Zoghbi

   Article

   Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

   Insert

   SNCA (SNCA Human)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-TO-hNGN2

   Plasmid

   #172115

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Article

   Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

   Inserts

   hNGN2 (NEUROG2 Human)
   puroR-T2A-mycNLS-mTagBFP2
   

   Use

   Tags

   T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and TRE3G

   Available since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLEX-MCS UBC promoter

   Plasmid

   #128045

   Purpose

   Lentiviral vector with UBC promoter

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR_Gal4UAS_dGFP_PGK_iRFP (pLZA042)

   Plasmid

   #203908

   Purpose

   Encodes dGFP downstream UAS promoter that's responsive to Gal4-VP64 activity in mammalian cells

   Depositor

   Jared Toettcher

   Article

   Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

   Insert

   Gal4UAS_dGFP_PGK_iRFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   5xUAS and PGK

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBetaActin-mNeonGreen-CAMSAP3

   Plasmid

   #191322

   Purpose

   Expresses the fluorescently labeled microtubule minus end marker CAMSAP3 to measure microtubule retrograde flow

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   mNeonGreen-CAMSAP3 (Camsap3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiSAMv2

    Plasmid

    #75112

    Purpose

    lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available since

    May 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSBbi-RN

    Plasmid

    #60519

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and neomycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSBtet-RN

    Plasmid

    #60503

    Purpose

    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and neomycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TCE & RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CKIIa-stGtACR2-FusionRed (AAV Retrograde)

    Viral Prep

    #105669-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-CKIIa-stGtACR2-FusionRed (#105669). In addition to the viral particles, you will also receive purified pAAV-CKIIa-stGtACR2-FusionRed plasmid DNA. CaMKIIa-driven, soma-targeted anion-conducting channelrhodopsin fused to FusionRed for optogenetic inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Ofer Yizhar

    Article

    Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

    Promoter

    CaMKII

    Tags

    FusionRed

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

14. pPSU10-30-50-100

    Plasmid

    #173838

    Purpose

    Coexpresses 10, 30, 50 and 100 kD Penn State ladder proteins

    Depositor

    Song Tan

    Article

    Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

    Insert

    STRHSTPAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-Myc-GFP

    Plasmid

    #83375

    Purpose

    Expression of Myc tagged GFP

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    Myc-GFP
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPSU20-40-60-80

    Plasmid

    #173839

    Purpose

    Coexpresses 20, 40, 60 and 80 kD Penn State ladder proteins

    Depositor

    Song Tan

    Article

    Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

    Insert

    STRHSTPABPACCBPv3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CAG-RdLight1 (AAV9)

    Viral Prep

    #125707-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-CAG-RdLight1 (#125707). In addition to the viral particles, you will also receive purified pAAV-CAG-RdLight1 plasmid DNA. CAG-driven expression of red genetically encoded dopamine sensor RdLight1. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Lin Tian

    Article

    Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

    Promoter

    CAG

    Tags

    None

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

18. pINDUCER21-MYB

    Plasmid

    #51305

    Purpose

    Expression of MYB after doxycycline administration

    Depositor

    George Daley

    Article

    Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

    Insert

    v-myb avian myeloblastosis viral oncogene homolog (MYB Human)
    

    Use

    Lentiviral

    Tags

    n/a

    Expression

    Mutation

    full length ORF

    Promoter

    Available since

    June 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. Myc-E-Syt1

    Plasmid

    #66833

    Purpose

    Mammalian Expression, Bacterial Expression

    Depositor

    Pietro De Camilli

    Article

    Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

    Insert

    E-Syt1 (ESYT1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET22b-PelB-6H-Ag43-160N-sfGFP

    Plasmid

    #172886

    Purpose

    Display sfGFP protein on E. coli cell surface

    Depositor

    Urartu Seker

    Article

    Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

    Inserts

    SfGFP
    Ag43, partial (flu )
    

    Use

    Tags

    HHHHHH and TEV recognition site (ENLYFQG)

    Expression

    Bacterial

    Mutation

    Deleted 1-160 (N terminal)

    Promoter

    T7 Promoter

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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