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Neurl1Rm-Flag pcDNA3.1

Plasmid

#17319

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Use

RNAi

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pTV-Nanog_2-5prime-1000-24MS96T-3F_NeoR

Plasmid

#174882

Purpose

Targeting vector for generating Nanog STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

STREAMING-tag with homology arms for Nanog

Use

Mouse Targeting

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pINDUCER20-Src(Y527F)

Plasmid

#215464

Purpose

Dox-inducible lentiviral transfer vector with Src constitutively-active mutant (Y527F)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Src (SRC Chicken)

Use

Lentiviral

Mutation

Y527F (constitutively-active)

Promoter

mCMV, Doxycycline-inducible

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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myc-UVRAG

Plasmid

#86736

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

Insert

human UVRAG (UVRAG Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV5 MEKK2 WT

Plasmid

#12182

Depositor

Gary Johnson

Article

Blank et al J Biol Chem. 1996 Mar 8. 271(10):5361-8.

Insert

MEKK2 (Map3k2 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
TIMP1-bio-His

Plasmid

#52089

Purpose

Expresses full-length Metalloproteinase inhibitor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

TIMP1 (TIMP1 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG MCP-VP64

Plasmid

#92357

Purpose

CAG-driven ubiquitous expression of MCP-VP64. MCP is bacteriophage MS2 coat protein, VP64 is transcriptional activation domain. Part of SAM-mediated gene activation system in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

MCP-VP64

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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ULBP3-bio-His

Plasmid

#51859

Purpose

Expresses full-length NKG2D ligand 3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

ULBP3 (ULBP3 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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TIMP3-bio-His

Plasmid

#53418

Purpose

Expresses full-length Metalloproteinase inhibitor 3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

TIMP3 (TIMP3 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-DCX-2A-mCherry

Plasmid

#118290

Purpose

Expresses human DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells.

Depositor

Vance Lemmon

Article

Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

Insert

doublecortin (DCX Human)

Use

AAV

Tags

2A peptide and mCherry

Expression

Mammalian

Promoter

CMV with beta global intron

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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NgΔIQ FUNgDIQGW

Plasmid

#92232

Purpose

Lentiviral expression of human Ng with deletion mutation

Depositor

Weifeng Xu

Article

Hwang et al Biol Psychiatry. 2021 Feb 1;89(3):256-26

Insert

Ng (NRGN Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

delta IQ

Promoter

hUbC

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pTV-Sox2_5prime-1000-24MS96T-3F_NeoR

Plasmid

#174884

Purpose

Targeting vector for generating Sox2 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

STREAMING-tag with homology arms for Sox2

Use

Mouse Targeting

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pRSV-CaMKIV

Plasmid

#45062

Purpose

Expression of CaMKIV

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

Cam Kinase IV (Camk4 Mouse)

Expression

Mammalian

Mutation

*

Promoter

RSV

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-LacR-TopBP1

Plasmid

#31313

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al J Biol Chem. 2011 Jun 3. 286(22):19229-3

Insert

TopBP1 (TOPBP1 Human)

Tags

FLAG and LacR

Expression

Mammalian

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
THOC1

Plasmid

#156081

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

THOC1 (THOC1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pRExT2A-CT-BmNG

Plasmid

#232207

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mNeongreen-3xTy-T2A-BSD-3xTy

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

mNeongreen

Expression

Bacterial

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pRExT2A-CT-PmNG

Plasmid

#232201

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mNeongreen-3xTy-T2A-PAC-3xTy

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

mNeongreen

Expression

Bacterial

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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Dynamin-I-K44A-6His/Flag

Plasmid

#32696

Depositor

Robert Lefkowitz

Article

Ahn et al J Biol Chem. 2002 Jul 19;277(29):26642-5

Insert

Dynamin I (Dnm1 Rat)

Tags

Flag and His6

Expression

Mammalian

Mutation

K44A

Promoter

CMV

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pRExT2A-CT-PmSc

Plasmid

#232549

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mScarlet-3xTy-T2A-PAC-3xTy

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

mScarlet

Expression

Bacterial

Mutation

None

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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Type I inositol-1,4,5-trisphosphate 5-phosphatase in pcDNA3

Plasmid

#121976

Purpose

transfection studies in eukaryotic cells such as COS-7 cells

Depositor

Christophe Erneux

Article

De Smedt et al J Biol Chem. 1996 Apr 26;271(17):10419-2

Insert

Type I Inositol-1,4,5-trisphoosphate 5-phosphatase (INPP5A Human)

Expression

Mammalian

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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