We narrowed to 3,644 results for: ORF1

Showing: 3541 - 3560 of 3644 results

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pBIG1a_3xFlag-6His-nsp14 (SARS-CoV-2)

Plasmid

#169163

Purpose

Baculoviral transfer vector to express SARS-CoV-2 nsp14 in insect cells

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

3xFlag-6His-nsp14 (ORF1ab Synthetic, SARS-CoV-2)

Tags

3xFlag-6His

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEAHISSIRV131

Plasmid

#97053

Purpose

Expressing Sulfolobus islandicus rudivirus (SIRV) orf 131 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

SIRV orf 131

Tags

N-terminal TEV protease cleavable 6xHis tag

Expression

Bacterial

Mutation

none

Promoter

T7

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_FAM196A_p.Q46R

Plasmid

#81451

Purpose

Gateway Donor vector containing FAM196A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FAM196A (FAM196A Human)

Use

Gateway entry vector

Mutation

Q46R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_FAM196A_p.R399Q

Plasmid

#81411

Purpose

Gateway Donor vector containing FAM196A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FAM196A (FAM196A Human)

Use

Gateway entry vector

Mutation

R399Q

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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AK9 gRNA (BRDN0001146911)

Plasmid

#77689

Purpose

3rd generation lentiviral gRNA plasmid targeting human AK9

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AK9 (AKD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AK9 gRNA (BRDN0001162330)

Plasmid

#77690

Purpose

3rd generation lentiviral gRNA plasmid targeting human AK9

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AK9 (AKD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AK9 gRNA (BRDN0001162237)

Plasmid

#77691

Purpose

3rd generation lentiviral gRNA plasmid targeting human AK9

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AK9 (AKD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AK9 gRNA (BRDN0001147860)

Plasmid

#77692

Purpose

3rd generation lentiviral gRNA plasmid targeting human AK9

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AK9 (AKD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PB-TRE-OKSM

Plasmid

#204498

Purpose

Expresses Oct4, Klf4, Sox2 and c-Myc by tet-on promoter in mammalian cells

Depositor

Wataru Fujii

Article

Matsuya et al Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.

Inserts

OCT4 (POU5F1 Human)
KLF4 (KLF4 Human)
SOX2 (SOX2 Human)

Expression

Mammalian

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pKP332 (Lenti-OSK1)

Plasmid

#21627

Depositor

Tim Townes

Article

Chang et al Stem Cells. 2009 May . 27(5):1042-9.

Insert

hOCT4 - 2A - hSOX2 - 2A - hKLF4 (POU5F1, SOX2, KLF4 Porcine teschovirus-1, Human)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

Nov. 12, 2009

Availability

Academic Institutions and Nonprofits only

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MOS

Plasmid

#64120

Purpose

A modified episomal (EBNA1/oriP) vector expressing human OCT4 and SOX2 genes

Depositor

Linzhao Cheng

Article

Chou et al Stem Cells Transl Med. 2015 Mar 5. pii:

Insert

OCT4-2A-SOX2 (POU5F1, SOX2 Human)

Tags

HA and Myc

Expression

Mammalian

Promoter

SFFV

Available Since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pTO_CHMP4B_LAP

Plasmid

#101850

Purpose

Tet-inducible expression of human CHMP4B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration

Depositor

Daniel Gerlich

Article

Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d

Insert

Charged Multivesicular Body Protein 4B (CHMP4B Human)

Use

Gateway cloning, flp-frt recombination, tet-induc…

Tags

Localization and Affinity Purification (LAP) tag

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-SARS-CoV-2_NSP3-VA

Plasmid

#191214

Purpose

Lentiviral expression of SARS-CoV-2 NSP3 in mammalian cells

Depositor

Mohan Babu

Article

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

Non structural protein 3 (ORF1ab SARS CoV-2)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pEP4 E02S EN2K

Plasmid

#20925

Purpose

used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Nanog and Klf4

Depositor

James Thomson

Article

Yu et al Science. 2009 Mar 26.

Insert

Oct4 and Sox2; Nanog and Klf4 (SOX2, POU5F1, NANOG, KLF4 Human)

Use

Episomal/ebna

Available Since

May 20, 2009

Availability

Academic Institutions and Nonprofits only

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pEP4 E02S EN2L

Plasmid

#20922

Purpose

used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Nanog and Lin28

Depositor

James Thomson

Article

Yu et al Science. 2009 Mar 26.

Insert

Oct4 and Sox2; Nanog and Lin28 (POU5F1, SOX2, NANOG, LIN28A Human)

Use

Episomal/ebna

Available Since

May 20, 2009

Availability

Academic Institutions and Nonprofits only

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pGL3-human OCT4 PE-SV40-Luc

Plasmid

#52415

Purpose

Luciferase reporter plasmid for PE OCT4 enhancer activity

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

Proximal element of hOCT4 promoter (POU5F1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pGL3-human OCT4 DE-SV40-Luc

Plasmid

#52414

Purpose

Luciferase reporter plasmid for DE OCT4 enhancer activity

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

Distal element of human OCT4 promoter (POU5F1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pK27Sumo_His-SUMO-nsp10-nsp16 fusion (SARS-CoV-2)

Plasmid

#169191

Purpose

To express nsp10-nsp16 fusion protein in E. coli

Depositor

John Diffley

Article

Basu et al Biochem J. 2021 Jul 16;478(13):2481-2497

Insert

14His-SUMO-nsp10-GSGSGS-nsp16 (ORF1ab Synthetic, SARS-CoV-2)

Tags

14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T5

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-SARS-CoV-2_NSP16-VA

Plasmid

#191215

Purpose

Lentiviral expression of SARS-CoV-2 NSP16 in mammalian cells

Depositor

Mohan Babu

Article

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

Non structural protein 16 (ORF1ab SARS CoV-2)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_CMVTRE3G_LYSET-Isoform2_Flag

Plasmid

#191999

Purpose

Lentiviral vector to generate flag-tagged LYSET(TMEM251)-isoform2 stable expressing cell line under CMVTRE3G promoter

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-Isoform2 (LYSET Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMVTRE3G

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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