We narrowed to 6,169 results for: pCas
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Plasmid#207524PurposePlasmid for CRISPRi; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpRDepositorInsertxylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR
UsePseudomonas vectorAvailable SinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLSC-5
Plasmid#62889PurposeLenti vector for expression of inducible split Cas9DepositorInsertsSpCas9(574-1368)
SpCas9(2-573)
UseCRISPR and LentiviralTagsFFBP12, FRB, NES PTK2, NLS SV40, and P2AExpressionMammalianMutation*see comment belowPromoterEFSAvailable SinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNuc-cis
Plasmid#202796PurposeRK2-based conjugative plasmid containing the TevSpCas9 dual-endonuclease for targeted bacterial killingDepositorInsertTevSpCas9, sgRNA cassette, oriT, CenArsHis
ExpressionBacterialAvailable SinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC0052-REPAIR non-targeting guide clone into pC0043
Plasmid#103868PurposeNon-targeting guide RNA for use with PspCas13b. Useful as a control for RNA editing and RNA knockdown experiments.DepositorInsertnon-targeting guide RNA
UseCRISPRExpressionMammalianAvailable SinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-sgRNA-com-BoxB-vector
Plasmid#132556PurposeExpresses dCas9-DMNT3a-DMNT3l fusion protein, lambda N22-KRAB fusion protein, and sgRNA scaffold with com and BoxB replacing the Tetraloop and the loop 2 respectively.DepositorInsertsdCas9-DMNT3a-DMNT3l (DNMT3A Synthetic)
Lambda N22-KRAB
sgRNA acaffold with com and BoxB replacing the Tetraloop and the loop 2.
ExpressionMammalianAvailable SinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Cas9-NRTH
Plasmid#136925PurposeExpresses Cas9-NRTH in mammalian cellsDepositorInsertCas9-NRTH
UseCRISPRExpressionMammalianMutationsee manuscriptPromoterCMVAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmCherry_gRNA
Plasmid#80457PurposegRNA expression vector with mCherry transfection controlDepositorInsertssp Cas9 gRNA
mCherry
UseCRISPRExpressionMammalianPromoterU6 and chicken β-actin promoterAvailable SinceAug. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pH-A3A-PBE
Plasmid#119774PurposeConversion of C to T in rice plants transformationDepositorInsertAPOBEC3A-nCas9-NLS-UGI-NLS
TagsNLS (flanking UGI)ExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9Promotermaize Ubiquitin-1Available SinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330A_D10A-1x2
Plasmid#58772PurposeExpresses Cas9 nickase and gRNADepositorInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTags3xFLAGExpressionMammalianMutationD10A nickase-converting mutationPromoterCBhAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
px459 VRER
Plasmid#101716PurposesgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)DepositorInsertSpCas9 VRER
Tags3xFLAG-NLS and NLSExpressionMammalianMutationVRER (D1135V, G1218R, R1335E and T1337R)Available SinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT03
Plasmid#223375PurposeT-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.DepositorInsert2x35s-SpCas9-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
sABE v3.22_C
Plasmid#209554PurposepCMV-SV40_NLS-2xFKBP3-TadA-8e(77-167)-SpCas9(D10A)-SV40_NLS-BGHpADepositorInsert2xFKBP-TadA-8e(77-167)-SpCas9(D10A)
ExpressionMammalianPromoterpCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT04
Plasmid#223376PurposeT-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.DepositorInsertZmUbi-SpCas9-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBWB419
Plasmid#140637PurposeRSF1010-Cas9-pcaHG gRNA. Vector with constitutive Cas9 with gRNA targeting pcaHG locus.DepositorInsertCas9
ExpressionBacterialAvailable SinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Cas9-NRCH
Plasmid#136926PurposeExpresses Cas9-NRCH in mammalian cellsDepositorInsertCas9-NRCH
UseCRISPRExpressionMammalianMutationsee manuscriptPromoterCMVAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT05
Plasmid#223377PurposeT-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.DepositorInsertZmUbi-SpCas9-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
c3GIC9
Plasmid#62191Purposecol1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 expression. Contains NsiI cloning site for U6-sgRNA cassettesDepositorInserthumanized S. Pyogenes Cas9
UseCRISPRTags3x FLAGPromoterTRE3GAvailable SinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
nos-Cas9.874Z1
Plasmid#112685PurposeExpress hSpCas9 under nanos promoterDepositorInserthSpCas9
Tags3x FLAG, EGFP, and NLSExpressionInsectMutationHumanized Cas9 bacterial sequencePromoternanosAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0051-W85X REPAIR targeting guide cloned into pC0043
Plasmid#103867PurposeGuide RNA expression plasmid for dPspCas13b that can be used to direct ADAR deaminase domain activity to the W85X mutation in the luciferase reporter plasmid pC0038DepositorInsertW85X targeting guide RNA
UseCRISPRExpressionMammalianAvailable SinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
SiC-V1
Plasmid#133041PurposeSiC-V1 vector with SpCas9 gene and dTomato reporter. sgRNA targeting a gene of interest can be cloned downstream of U6 promoter.DepositorInsertSpCas9
UseCRISPR and LentiviralTagsdTomatoAvailable SinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only