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pLX317 YAP1

Plasmid

#193683

Purpose

Constitutive lentiviral expression of YAP1

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL11060

Plasmid

#68264

Purpose

Level 1 Golden Gate Cassette: Cas9 expression cassette for plants (exemplified in Brassica oleracea)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter/5UTR: CsVMV+ CDS:SpCas9 + 3UTR/terminator:35s

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pJOP-HTT-HR18Q

Plasmid

#87228

Purpose

HTT HR arms with 18 CAG repeats and piggyBac selection cassette

Depositor

Mahmoud Pouladi

Article

Xu et al Stem Cell Reports. 2017 Feb 21. pii: S22

Insert

1.7kb HTT 5' homology arm, 2.5kb HTT 3' homology arm

Expression

Mammalian

Promoter

EF1a driving selection cassette

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pICOZ-SB100X[EF1α-CD19 4-1BB]

Plasmid

#218328

Purpose

Donor plasmid of SB100X Transposon with CD19 CAR mammalian expression cassette

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

CD19 CAR mammalian expression cassette

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shYAP1-1

Plasmid

#193692

Purpose

Constitutive lentiviral expression of YAP1 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLX317 CALM2

Plasmid

#193684

Purpose

Constitutive lentiviral expression of CALM2

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

CALM2 (CALM2 Human)

Use

Lentiviral

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pQLL-Tc1-1_Xt[EF1α-copGFP]

Plasmid

#218325

Purpose

Donor plasmid of Tc1-1_Xt Transposon with copGFP mammalian expression cassette

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

copGFP mammalian expression cassette

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLL5.0-Anillin shRNA

Plasmid

#187270

Purpose

Lentiviral expression of shRNA targeting Anillin

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

shRNA for Anillin (ANLN Human)

Use

Lentiviral

Tags

EGFP

Promoter

U6

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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TPC1(ΔN69)-mCherry

Plasmid

#135184

Purpose

TPC1 with truncated N-terminus, tagged with mCherry on C terminus.

Depositor

Antony Galione

Article

Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

Insert

TPC1 (Tpcn1 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Deleted amino acids 1-69

Available Since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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PB-GG-OCT4-1-5-PGK-Puro

Plasmid

#102893

Purpose

PiggyBac transposon system construct with 5 concatenated U6 promoter driven transcriptional cassettes for the activation of OCT4. Contains PGK-puro selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

OCT4-gRNAs-1-5-PGK-puro (POU5F1 )

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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TPC2(ΔN49)-mCherry

Plasmid

#135185

Purpose

TPC2 with truncated N-terminus, tagged with mCherry on C terminus.

Depositor

Antony Galione

Article

Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

Insert

TPC2 (Tpcn2 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Deleted amino acids 1-49

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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B52 + SMARCAL1 sgSTOP

Plasmid

#100715

Purpose

B52 plasmid expressing SMARCAL1 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting SMARCAL1 (cloned using BbsI)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE3_ mTFP1- Vamp2

Plasmid

#201214

Purpose

AAV expression of TRE(TRE3G)-driven, Vamp2-conjugated mTFP1 expression for fluorescent labling of axon terminals

Depositor

Tetsuo Yamamori

Article

Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

Insert

mTFP1 (from addgene #54613)

Use

AAV

Tags

mouse Vamp2

Expression

Bacterial and Mammalian

Promoter

TRE3G

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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B52 + SPRTN sgSTOP

Plasmid

#100709

Purpose

B52 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting SPRTN (cloned using BbsI)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pQLL-Tc1-8B_DR[EF1α-copGFP]

Plasmid

#218327

Purpose

Donor plasmid of Tc1-8B_DR Transposon with copGFP mammalian expression cassette

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

copGFP mammalian expression cassette

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG-JAK1Gly590Arg

Plasmid

#174573

Purpose

Human FLAG-tagged JAK1 Gly590Arg mutant expression (N-term. tag) (CMV prom.)

Depositor

Mathew Garnett

Article

Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

Insert

JAK1Gly590Arg

Tags

FLAG

Expression

Mammalian

Mutation

Gly590Arg

Promoter

CMV

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pWPT-/mEGFP-1T-IRES-mCherry

Plasmid

#190190

Purpose

Lentiviral expression vector with altered Kozak sequence to direct EGFP expression. mCherry expression is regulated by an IRES.

Depositor

Alessandro Quattrone

Article

Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

Inserts

mEGFP
mCherry

Use

Lentiviral

Mutation

changed EGFP Kozak sequence inserting a C>T mo…

Promoter

EIF1-short

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Gal4-entry+NLS

Plasmid

#128013

Purpose

Vector containing the Gal4-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer

Depositor

Julius Brennecke

Article

Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

Insert

Gal4 DBD-SV40NLS-3x-Flag

Use

Gal4 entry vector with nls to generate tethering …

Tags

Gal4-DBD-SV40NLS-3xFlag

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-tetpA-hNEUROG2-iRPT

Plasmid

#140764

Purpose

Expresses iRFP713, PuroR and rtTAM2 in mammalian cells, with TRE-hNEUROG2

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Neurosci Res. 2019 Oct 3. pii: S0168-010

Inserts

iRFP713
PuroR
rtTAM2
NEUROG2 (NEUROG2 Human)

Expression

Mammalian

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-T0-Flag-HROB

Plasmid

#135298

Purpose

Expresses Flag-tagged HROB in mammalian cells

Depositor

Daniel Durocher

Article

Hustedt et al Genes Dev. 2019 Oct 1;33(19-20):1397-141

Insert

HROB (HROB Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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