Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 16,799 results for: puromycin

Showing: 3561 - 3580 of 16799 results

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pSuperCRISPR-sgRNA-RL69-TTTC

Plasmid

#215855

Purpose

This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

Depositor

Kumiko Ui-Tei

Article

Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

Insert

sgRNA-RL69 with TTTC stretch

Use

Retroviral

Expression

Mammalian

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pSuperCRISPR-sgRNA-RL69-TCTT

Plasmid

#215853

Purpose

This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

Depositor

Kumiko Ui-Tei

Article

Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

Insert

sgRNA-RL69 with TCTT stretch

Use

Retroviral

Expression

Mammalian

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pSuperCRISPR-sgRNA-RL69-TTCT

Plasmid

#215854

Purpose

This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

Depositor

Kumiko Ui-Tei

Article

Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

Insert

sgRNA-RL69 with TTCT stretch

Use

Retroviral

Expression

Mammalian

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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Halo-LC3B HRD

Plasmid

#207539

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous LC3B locus in human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag with internal PuroR cassette flanked by human LC3B locus sequences

Tags

HaloTag

Expression

Mammalian

Promoter

Endogenous

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pX459-canis_PTGS2-4

Plasmid

#184730

Purpose

COX2-KO in MDCK

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the dog COX2 gene.

Use

CRISPR

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX459-homo_PLA2G4A-1

Plasmid

#184735

Purpose

cPLA2-KO in HeLa

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the human cPLA2 gene.

Use

CRISPR

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX459-homo_PLA2G4A-2

Plasmid

#184736

Purpose

cPLA2-KO in HeLa

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the human cPLA2 gene.

Use

CRISPR

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX459-canis_PTGS1-3

Plasmid

#184727

Purpose

COX1-KO in MDCK

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the dog COX1 gene.

Use

CRISPR

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX459-canis_PTGS1-4

Plasmid

#184728

Purpose

COX1-KO in MDCK

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the dog COX1 gene.

Use

CRISPR

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX459-canis_PTGS2-3

Plasmid

#184729

Purpose

COX2-KO in MDCK

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the dog COX2 gene.

Use

CRISPR

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPBpuro-UbC-4493NES(Booster-PKA)

Plasmid

#184711

Purpose

PKA biosensor

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

Booster-PKA

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-h.Rhno1 T52A (B)-C-Myc-DDK-P2A-Puro

Plasmid

#211621

Purpose

Rhno1 T52A

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

hRHNO1 (RHNO1 Human)

Tags

myc-flag

Expression

Mammalian

Mutation

T52A

Promoter

CMV

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-h.Rhno1 S51A (A)-C-Myc-DDK-P2A-Puro

Plasmid

#211620

Purpose

Rhno1 S51A

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

hRHNO1 (RHNO1 Human)

Tags

myc-flag

Expression

Mammalian

Mutation

S51A

Promoter

CMV

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-h.Rhno1 S163A (E)-C-Myc-DDK-P2A-Puro

Plasmid

#211624

Purpose

Rhno1 S163A

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

hRHNO1 (RHNO1 Human)

Tags

myc-flag

Expression

Mammalian

Mutation

S163A

Promoter

CMV

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-h.Rhno1 S162A (D)-C-Myc-DDK-P2A-Puro

Plasmid

#211623

Purpose

Rhno1 S162A

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

hRHNO1 (RHNO1 Human)

Tags

myc-flag

Expression

Mammalian

Mutation

S162A

Promoter

CMV

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-h.Rhno1 Cdk1mut7A-C-Myc-P2A-Puro

Plasmid

#211617

Purpose

Rhno1 Cdk1mut7A

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

hRHNO1 (RHNO1 Human)

Expression

Mammalian

Mutation

S58A, S98A, S133A, T159A, T198A, S191A, and T202A

Promoter

CMV

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-h.Rhno1 S164A (F)-C-Myc-DDK-P2A-Puro

Plasmid

#211625

Purpose

Rhno1 S164A

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

hRHNO1 (RHNO1 Human)

Tags

myc-flag

Expression

Mammalian

Mutation

S164A

Promoter

CMV

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-h.Rhno1 S178A (G)-C-Myc-DDK-P2A-Puro

Plasmid

#211626

Purpose

Rhno1 S178A

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

hRHNO1 (RHNO1 Human)

Tags

myc-flag

Expression

Mammalian

Mutation

S178A

Promoter

CMV

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v2 sgUCKL1

Plasmid

#211523

Purpose

Deletes UCKL1

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgUCKL1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v2 sgUCK2

Plasmid

#211522

Purpose

Deletes UCK2

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgUCK2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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Showing: 3561 - 3580 of 16799 results

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