We narrowed to 9,137 results for: set
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Plasmid#92431Purposemammalian expression of PAFAH1B1 R22 mutant. The protein is truncated and non functional (negative control).DepositorInsertPAFAH1B1 (PAFAH1B1 Human)
ExpressionMammalianMutationR22 (stop codon in position 22)PromoterCMVAvailable SinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
attB53_SNCB+_attB53
Plasmid#183614PurposeRMCE vector to shuttle puromycin resistance, anti-GFP SynNotch receptor, tagBFP and TRE-mCherry cassettes into attP50-flanked landing pad (Addgene 183609). All cassettes on sense (+) strand.DepositorInsertsLaG17 GFP nanobody SynNotch tTA
TRE-mCherry-SV40pA
tagBFP
UseRmceTagsMyc and human CD8a signal sequenceExpressionMammalianAvailable SinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPlacPbuCas13b-gRNA-T-MS2
Plasmid#184839PurposeExpression of a single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome and expression of PbuCas13b in bacteria.DepositorInsertsingle-spacer CRISPR array with spacer #1 targeting the MS2 phage genome, PbuCas13b nuclease
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23119 and lac promoterAvailable SinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
B*58:02:01
Plasmid#203262PurposeOverexpress HLA alleleDepositorInsertB*58:02:01 (HLA-B Human)
ExpressionMammalianAvailable SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
LN-entry+NLS
Plasmid#128011PurposeVector containing the lambdaN-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancerDepositorInsertLN-3xFlag-SV40NLS
UseLamdan entry vector with nls to generate tetherin…TagsLN-3xFlag-SV40NLSAvailable SinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
iBE3 CLYBL
Plasmid#174569Purposedoxycycline inducible BE3 expression for human CLYBL locus knock-inDepositorInsertBE3
ExpressionMammalianPromotertet ONAvailable SinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMx PylT C41CA_FLAG_Mx PylRS
Plasmid#140013PurposePlasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertMx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
TagsFLAGExpressionMammalianPromoterEF1Available SinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
A*01:03:01
Plasmid#203293PurposeOverexpress HLA alleleDepositorInsertA*01:03:01 (HLA-A Human)
ExpressionMammalianAvailable SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDRF'- AmTryoshka1;3-LS-F138I-T78H
Plasmid#100963PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFPDepositorInsertAmTryoshka1;3-LS-F138I-T78H (AMT1;3 Mustard Weed)
ExpressionYeastMutationF138I, T78HPromoterPMAAvailable SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
B*07:05:01
Plasmid#203268PurposeOverexpress HLA alleleDepositorInsertB*07:05:01 (HLA-B Human)
ExpressionMammalianAvailable SinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR P4-P1R-V5-6xHis
Plasmid#48346PurposeContributes the coding sequence of a V5-6xHis epitope tags cassette as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertV5 and 6xHis epitope tags cassette
UseGateway entry cloneMutationContains a Kozak sequence and initiation codon up…PromoternoneAvailable SinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAav-MCS-PQS1-3xFLAG
Plasmid#84883PurposerAAV-based template for genome engineering of protein C-termini containing PQS1 and 3xFLAG tags and a selection cassetteDepositorInsertMulticloning sites and selection cassette
UseAAVPromoterPGKAvailable SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
Gal4-entry
Plasmid#128014PurposeVector containing the Gal4-entry expression cassette and a separate expression cassette driving mCherry via the traffic jam enhancerDepositorInsertGal4-DBD-3xFlag
UseGal4 entry vectorTagsGal4-DBD-3xFlagAvailable SinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro-Exon4-PAFAH1B1
Plasmid#92433Purposeexpresses Cas9 and gRNA targeting PAFAH1B1DepositorAvailable SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Intron4-PAFAH1B1
Plasmid#92434Purposeexpresses Cas9 and gRNA targeting PAFAH1B1DepositorAvailable SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
WT MetNI in pBAD
Plasmid#118254PurposeWild Type Methionine ABC Transporter MetNI in pBAD Vector for Transport Activity AssayDepositorTags10x His Tag and Enterokinase Cleavage SiteExpressionBacterialPromoterArabinose Promoter and NoneAvailable SinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pN-iRS3GG
Plasmid#98858PurposeExpresses pheS cassette (in place of an antisense RNA probe, for golden gate cloning) in E. coli (kanR).DepositorInsertPheS cassette to be selectively replaced by unique asRNA probes
ExpressionBacterialPromoterpBADAvailable SinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDRF'- AmTryoshka1;3-LS-L255I
Plasmid#100964PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.DepositorAvailable SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDRF'- AmTryoshka1;3-GS-F138I
Plasmid#100965PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.DepositorAvailable SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDRF'- AmTryoshka1;3-GS-L255I
Plasmid#100966PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.DepositorAvailable SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only