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pWZL Neo Myr Flag AAK1

Plasmid

#20133

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

AAK1 (AAK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pDONR223-NPR2

Plasmid

#23479

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NPR2 (NPR2 Human)

Use

Gateway Cloning

Mutation

isoform missing aa905-962

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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SLF045: AAV-hSyn-eTsChR-GFP

Plasmid

#112280

Purpose

AAV based broad expression of eTsChR in neurons

Depositor

Adam Cohen

Article

Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

Insert

eTsChR

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

hSyn

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pEM705 Gsq

Plasmid

#109356

Purpose

GsX chimera with Gαq C-terminus

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gsq chimera

Tags

None

Expression

Mammalian

Promoter

CAG

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pZac2.1 GfaABC1D rM3D mCherry SV40

Plasmid

#92285

Purpose

AAV DREADD expression

Depositor

Baljit Khakh

Article

Chai et al Neuron. 2017 Aug 2;95(3):531-549.e9. doi

Insert

rM3D

Use

AAV

Tags

mCherry

Promoter

GfaABC1D

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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AAVS1-Tet-OsTIR1(WT)-V5

Plasmid

#158663

Purpose

Tet-OsTIR1(WT)-V5 donor for integration at the AAVS1 locus

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

Tet-OsTIR1(WT) AAVS1

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_BRAF_p.V600E

Plasmid

#82186

Purpose

Gateway Donor vector containing BRAF , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BRAF (BRAF Human)

Use

Gateway Cloning

Mutation

V600E

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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N1-mSin1.2-GFP

Plasmid

#72908

Purpose

GFP-tagged mSin1 (isoform 2)

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

Target of rapamycin complex 2 subunit MAPKAP1, isoform 2 (MAPKAP1 Human)

Tags

eGFP

Expression

Mammalian

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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Active Wnt8A-V5

Plasmid

#43818

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

WNT8A (WNT8A Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 EYFP H148Q

Plasmid

#25871

Depositor

Peter Haggie

Article

Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

Insert

EYFP H148Q

Expression

Mammalian

Mutation

EYFP from pEYFP (Clontech) with Histidine 148 to …

Available Since

Aug. 16, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3-deltaBamHI-human MIZ1

Plasmid

#74167

Purpose

Mammalian expression of human MIZ1

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

MIZ1 (ZBTB17 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only
pRK5-mWnt3

Plasmid

#42276

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt3 (Wnt3 Mouse)

Expression

Mammalian

Available Since

March 29, 2013

Availability

Academic Institutions and Nonprofits only
pMK425 (GAL-OsTIR1(F74A))

Plasmid

#140657

Purpose

GAL-OsTIR1(F74A)

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

GAL-OsTIR1(F74A)

Expression

Yeast

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pRRL-GFPAdGO-PGK-Neo

Plasmid

#136898

Purpose

Lentivirus for base editing activatable GFP expression in mammalian cells

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

GFPAdGO

Use

Lentiviral

Tags

2xNLS

Mutation

TGG>TGA

Promoter

SFFV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pY017 (pcDNA3.1-hPcCpf1)

Plasmid

#69989

Purpose

Expresses humanized PcCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hPcCpf1

Use

CRISPR

Tags

NLS & 3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pY012 (pcDNA3.1-hCMtCpf1)

Plasmid

#69984

Purpose

Expresses humanized CMtCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hCMtCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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Active Wnt9B-V5

Plasmid

#43821

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

WNT9B (WNT9B Human)

Tags

V5

Expression

Mammalian

Mutation

M106T

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 V5 His-TOPO-GluCl optalpha-mYFP L9'F (Opt α-GluClv2.0)

Plasmid

#47387

Purpose

allow pharmacologically induced silencing of electrical activity in CNS neurons upon exposure to the anthelmintic drug ivermectin

Depositor

Henry Lester

Article

Frazier et al J Biol Chem. 2013 May 29.

Insert

glutamate-gated chloride Channels

Expression

Mammalian

Mutation

Leu9 prime mutated to F (Phenylalanine in M3-M4 l…

Promoter

CMV

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR223-RPS6KA5

Plasmid

#23758

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RPS6KA5 (RPS6KA5 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
HotPETase_pET21b

Plasmid

#215407

Purpose

Expression construct for HotPETase gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

HotPETase

Expression

Bacterial

Mutation

S121E/D186H/R280A/P181V/S207R/S214Y/Q119K/S213E/N…

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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