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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 63,812 results for: cloning

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Showing: 3581 - 3600 of 63812 results

  7. pLenti-H2B-iRFP720

    Plasmid
    #128961
    Purpose
    Lentiviral plasmid for expression of far red iRFP720 nuclear marker
    Depositor
    Carlos Carmona-Fontaine
    Insert
    Human histone H2B (H2BC21 Human)
    Use
    Lentiviral
    Tags
    iRFP720
    Expression
    Mammalian
    Promoter
    hPGK
    Available since
    Aug. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    128961-plasmid-map-sequence-id-252385

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  11. KRAB-dCas9

    Plasmid
    #112195
    Purpose
    KRAB fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KRAB (ZNF10 Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-KRAB-dCas9-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112195-plasmid-map-sequence-id-213538

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  13. xCas9(3.6)-ABE(7.10)

    Plasmid
    #108387
    Purpose
    Mammalian expression vector for xCas9(3.6)-ABE
    Depositor
    David Liu
    Publication
    Hu et al Nature. 2018 Feb 28. pii: nature26155. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    xCas9(3.6)-ABE(7.10)
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …
    Available since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    108387-plasmid-map-sequence-id-207183

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  15. pKK-TEV-CyOFP1

    Plasmid
    #105799
    Purpose
    Expression of your protein of interest in fusion with orange fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.
    Depositor
    Andrzej Dziembowski
    Publication
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-CyOFP1
    Expression
    Mammalian
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105799-plasmid-map-sequence-id-203392

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  16. pDEST14-Cys-SpyCatcher002

    Plasmid
    #102829
    Purpose
    Bacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagents
    Depositor
    Mark Howarth
    Publication
    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi
    Insert
    Cys-SpyCatcher002
    Tags
    GSSGS, His6, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Cysteine residue in N-terminal region
    Promoter
    T7
    Available since
    Dec. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    102829-plasmid-map-sequence-id-200986

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Showing: 3581 - 3600 of 63812 results