We narrowed to 5,258 results for: pcdna3.1

1. MCP/PCP RNA Lantern

   Plasmid

   #226095

   Purpose

   RNA tracking

   Depositor

   Jennifer Prescher

   Article

   Halbers et al Nat Commun. 2024 Nov 18;15(1):9992. doi:

   Insert

   NLS-HA-MS2-SmBiT-IRES-PP7-LgBiT-FLAG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]

   Plasmid

   #227246

   Purpose

   All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cell

   Depositor

   Jae Seong Lee

   Article

   Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.

   Inserts

   Tet-On 3G transactivator protein
   Enhanced green fluorescent protein
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α and pTRE3G

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. VfAU1-LOV_P2A_casp9

   Plasmid

   #131224

   Purpose

   Expresses Ha-tagged VfAU1-LOV and myc-tagged casp9 (domains of Opto-casp9) separated by P2A site

   Depositor

   Harald Janovjak

   Article

   Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

   Insert

   VfAU1-LOV-HA_P2A_myc-casp9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC0079 RESCUE-S

   Plasmid

   #130662

   Purpose

   pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375A/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/S661T) V16S

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

   Insert

   dRanCas13b-ADAR2dd
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-NES-PhoCl-mCherry

   Plasmid

   #87690

   Purpose

   Express NES-PhoCl-mCherry in mammalian cells

   Depositor

   Robert Campbell

   Article

   Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   NES-PhoCl-mCherry
   

   Use

   Tags

   nuclear export signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTS2311-Tier1-spacer-PPGK-NLuc_TB

   Plasmid

   #169552

   Purpose

   Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-NanoLuc Luciferase - mTagBFP fusion
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. deltaNp63alpha-FLAG

   Plasmid

   #26979

   Depositor

   David Sidransky

   Article

   Chatterjee et al Cell Cycle. 2008 May 1. 7(9):1231-7.

   Insert

   ΔNP63alpha (TP63 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   dominant negative isoform of p63; aa 1–587

   Promoter

   Available since

   Dec. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. HaloTag-sec61b

   Plasmid

   #186959

   Purpose

   Express sec61b connected with N-terminus HaloTag

   Depositor

   Ke Xu

   Article

   Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

   Insert

   Sec61 (SEC61B Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Promoter

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA-RLuc8

   Plasmid

   #87121

   Purpose

   Mammalian expression vector expressing mutant version of the reporter gene-Renilla Luciferase (Rluc8), which is more stable in mouse serum and has more light output.

   Depositor

   Sanjiv Sam Gambhir

   Article

   Loening et al Protein Eng Des Sel. 2006 Sep;19(9):391-

   Insert

   Renilla Luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   A55T, C124A, S130A, K136R, A143M, M185V, M253L, a…

   Promoter

   CMV

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-HA-Sumo1

    Plasmid

    #21154

    Purpose

    Mammalian expression of human Sumo1 with HA tag

    Depositor

    Junying Yuan

    Article

    Terui et al J Biol Chem. 2004 Jul 2. 279(27):28257-6

    Insert

    pcDNA3-HA-Sumo1 (SUMO1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. Vinculin-venus

    Plasmid

    #27300

    Depositor

    Martin Schwartz

    Article

    Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

    Insert

    Vinculin (VCL Chicken)
    

    Use

    Tags

    Venus A206K

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Mfn1-Myc

    Plasmid

    #23212

    Depositor

    David Chan

    Article

    Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

    Insert

    Mfn1 (Mfn1 Mouse)
    

    Use

    Tags

    6xHis, 9xMyc, and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST-ORF-V2

    Plasmid

    #73638

    Purpose

    Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 2 (V2).

    Depositor

    Darren Saunders

    Article

    Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

    Type

    Empty backbone

    Use

    Tags

    Venus fragment 2 (V2)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST-ORF-V1

    Plasmid

    #73637

    Purpose

    Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 1 (V1)

    Depositor

    Darren Saunders

    Article

    Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

    Type

    Empty backbone

    Use

    Tags

    Venus fragment 1 (V1)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-Flag-H3.3-polyA

    Plasmid

    #59780

    Purpose

    Express Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    H3F3B (H3-3B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-Mm.cargo(Cre)

    Plasmid

    #174862

    Purpose

    Expresses mouse Peg10 cargoRNA encoding Cre recombinase

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    Cre flanked by MmPeg10 UTRs
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Halo-MTS

    Plasmid

    #124315

    Purpose

    Expresses mitochondria specific HaloTag protein

    Depositor

    Jin Wang

    Article

    Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

    Insert

    Halo tag
    

    Use

    Tags

    Mito specific seq

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. HaloTag-ER-3

    Plasmid

    #186960

    Purpose

    ER lumen localizaing HaloTag

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    CALR (CALR Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-Hs.cargo(Cre)

    Plasmid

    #174863

    Purpose

    Expresses human Peg10 cargoRNA encoding Cre recombinase

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    Cre flanked by HsPeg10 UTRs
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA-FLAG-Bbs4

    Plasmid

    #128868

    Purpose

    For mammalian expression of mouse Bbs4

    Depositor

    Aimin Liu

    Article

    Ye et al Proc Natl Acad Sci U S A. 2014 Feb 11;11

    Insert

    Bbs4 (Bbs4 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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