We narrowed to 24,898 results for: Spr

1. pScalps eSpCas9_Zsgreen gRNA scaffold

   Plasmid

   #188449

   Purpose

   Expression of eSpCas9 tagged with Zsgreen

   Depositor

   Ajit Chande

   Article

   Mishra et al Mol Ther Nucleic Acids. 2022 Mar 8;28:20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Promoter

   EF1-alpha

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pY094

   Plasmid

   #84743

   Purpose

   Expresses huAsCpf1-T2A-GFP and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huAsCpf1
   GFP
   

   Tags

   3xHA, NLS, and T2A

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJMP1337

   Plasmid

   #119270

   Purpose

   for cloning new sgRNAs

   Depositor

   Oren Rosenberg, Jason Peters, Carol Gross

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA pDEST40 Cas9-POLD3

   Plasmid

   #183194

   Purpose

   Expression clone

   Depositor

   Emma Haapaniemi

   Article

   Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

   Insert

   POLD3
   

   Use

   CRISPR

   Tags

   Cas9WT

   Expression

   Mammalian

   Mutation

   n/a

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSPneogRNA241510+MT

   Plasmid

   #84292

   Purpose

   Express LdPBK_241510.1 and LdMT targeting gRNAs simutaneously

   Depositor

   Greg Matlashewski

   Article

   Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

   Insert

   LdBPK_241510.1 targeting gRNA and LdMT targeting gRNA
   

   Use

   CRISPR; Leishmania donovani

   Promoter

   L. donovani ribosome RNA promoter

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-MH6-RspCas13d_RspCasWYL1

   Plasmid

   #108304

   Purpose

   Expresses E. coli codon optimized RspCas13d and RspCasWYL1 in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

   Inserts

   RspCas13d
   RspWYL1
   

   Use

   CRISPR

   Tags

   mH6 tag

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPEY-GAL-Kan-ADE2

   Plasmid

   #232105

   Purpose

   Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   ADE2 gRNA and repair template
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   Repair template introduces C at nt 466 of ADE2 to…

   Promoter

   GAL7

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcU6_3 sgRNA

   Plasmid

   #92359

   Purpose

   Chicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   chick U6.3 promoter and gRNA cloning cassette
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   chick U6.3

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. NAE1 G8.3 gRNA

   Plasmid

   #90777

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human NAE1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   NAE1 (Guide Designation G8.3)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCas3-Km

    Plasmid

    #207999

    Purpose

    Expresses all necessary components of Type I-C CRISPR-Cas system

    Depositor

    Pablo Ivan Nikel

    Article

    Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

    Inserts

    RhaR (rhaR )
    RhaS (rhaS )
    pRhaBAD
    I-C crRNA
    Cas3
    Cas5
    Cas8
    

    Expression

    Mammalian

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTE4396

    Plasmid

    #74041

    Purpose

    Expresses human codon-optimized AsCpf1 and As crRNA.

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Inserts

    As crRNA
    AsCpf1
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Promoter

    CMV and human U6

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCFD3-ovoD1

    Plasmid

    #111142

    Purpose

    Expresses U6:3-sgRNA targeting ovoD1 mutation in Drosophila melanogaster

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al G3 (Bethesda). 2018 Jul 31;8(8):2749-275

    Insert

    pCFD3-ovoD1 (ovo Fly)
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    U6:3

    Available Since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKRG3-mU6-PUb-hSpCas9

    Plasmid

    #162162

    Purpose

    Expresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

    Depositor

    Charles Rice

    Article

    Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

    Inserts

    hSpCas9
    sgRNA
    

    Use

    CRISPR

    Tags

    N/A

    Expression

    Insect

    Promoter

    Ae. aegypti PUb and Ae. aegypti U6

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-AsCpf1-Blast

    Plasmid

    #84750

    Purpose

    Expresses human codon-optimized AsCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

    Insert

    AsCpf1
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS-3xHA (C terminal on insert)

    Expression

    Mammalian

    Promoter

    EFS-NS

    Available Since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZR040_Lenti-U6-sgCtrl-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

    Plasmid

    #180266

    Purpose

    Lentiviral expression vector for CRISPRa-SAM with non-targeting control sgRNA

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    No-Target Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-ABE7.10-P2A-GFP

    Plasmid

    #162509

    Purpose

    Express ABE7.10-P2A-GFP by CMV promoter in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Shin et al Nucleic Acids Res. 2021 Feb 26;49(4):239

    Insert

    ABE7.10-P2A-GFP
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSNR52-sgTEF1

    Plasmid

    #46922

    Purpose

    Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TEF1 promoter

    Depositor

    Jonathan Weissman, Stanley Qi

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Insert

    sgTEF1 promoter (TEF1 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_KanR neo

    Plasmid

    #167901

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Mutation

    D10A, D839A, H840A, N863A

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSG4K5

    Plasmid

    #74492

    Purpose

    For cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2

    Insert

    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. 3xFLAG-dCas9/pMXs-puro

    Plasmid

    #51240

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS (nuclear localization signal)

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available Since

    Aug. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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