We narrowed to 59,428 results for: cloning

1. psBIG1d

   Plasmid

   #229960

   Purpose

   bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. psBIG1e

   Plasmid

   #229961

   Purpose

   bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. psBIG1c

   Plasmid

   #229959

   Purpose

   bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pnYC-NpM-HsNot10_25-707_AF

   Plasmid

   #148678

   Purpose

   Bacterial Expression of HsNot10_25-707

   Depositor

   Elisa Izaurralde

   Insert

   HsNot10_25-707 (CNOT10 Human)
   

   Expression

   Bacterial

   Mutation

   one silent mutation compared to the sequence give…

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. p667-UBC-hcRed-V5_IDG-K

   Plasmid

   #135239

   Purpose

   Gateway destination clone of hcRed (as control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   hcRed-V5
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. p663-UBC-V5-eGFP_IDG-K

   Plasmid

   #135265

   Purpose

   Gateway destination clone of eGFP (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   V5-eGFP
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-CMV-Flag-hcRed_IDG-K

   Plasmid

   #135274

   Purpose

   Gateway destination clone of hcRed (as control) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics

   Depositor

   Ben Major

   Insert

   Flag-hcRed
   

   Use

   Lentiviral; Gateway destination

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACY.HRF_T7_term_HR1

   Plasmid

   #111981

   Purpose

   Sub cloning vector position 1

   Depositor

   Stephen Harrison

   Article

   Jenni et al Science. 2018 May 4;360(6388):552-558. d

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. TJP3

   Plasmid

   #25310

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   TJP3:G508-L808:cH6 (TJP3 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains amino acids G508-L808

   Available Since

   April 4, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

10. Pf-FKBP-TPR

    Plasmid

    #25582

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    Pf-FKBP-TPR (FKBP35 Plasmodium Falciparum)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acid residues 128-304.

    Available Since

    Oct. 25, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

11. SGF29 (3MEW)

    Plasmid

    #25320

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SGF29_23 (SGF29 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids 129-291

    Available Since

    Aug. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE

    Plasmid

    #138148

    Purpose

    Expresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    CasRx
    

    Use

    Lentiviral

    Tags

    NES

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

    Plasmid

    #138147

    Purpose

    Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    CasRx
    

    Use

    Lentiviral

    Tags

    NLS

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid

    #73797

    Purpose

    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and EF1A

    Available Since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. lenti sgRNA(MS2)_puro backbone

    Plasmid

    #73795

    Purpose

    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and EF1A

    Available Since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. lenti sgRNA(MS2)_zeo backbone

    Plasmid

    #61427

    Purpose

    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and EF1A

    Available Since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MSCV-Luciferase-IRES-NrasG12D

    Plasmid

    #60834

    Purpose

    Mouse NrasG12D was amplified and mutated by PCR from IMAGE clone 6475312 (Invitrogen) and cloned downstream from the IRES; subsequently Luciferase was cloned upstream of the IRES

    Depositor

    Scott Lowe

    Article

    Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

    Insert

    NrasG12D (Nras Mouse)
    

    Use

    Retroviral

    Available Since

    Nov. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR221-JUN

    Plasmid

    #79512

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human JUN CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    JUN (JUN Human)
    

    Use

    Multisite gateway entry clone

    Tags

    HA Tag

    Promoter

    no Promoter

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGWB501

    Plasmid

    #74843

    Purpose

    Gateway cloning compatible binary vector for simple cloning of gene.

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    No Promoter

    Available Since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. TMPRSS2

    Plasmid

    #53887

    Purpose

    Expression of TMPRSS2

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Has Service

    Cloning Grade DNA

    Insert

    TMPRSS2 (TMPRSS2 Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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