We narrowed to 11,632 results for: Vars

1. pAAV-GFAP104-melanopsin-mCherry

   Plasmid

   #122630

   Purpose

   AAV-mediated expression of melanopsin-mCherry under the GFAP promoter

   Depositor

   Edward Boyden

   Article

   Mederos et al Glia. 2019 May;67(5):915-934. doi: 10.10

   Insert

   melanopsin-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   GFAP104

   Available Since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR-HLA-A2_GFP

   Plasmid

   #85161

   Purpose

   HLA.A2_GFP fusion with 2 glycines in between

   Depositor

   Tone Fredsvik Gregers, Sébastien Walchli

   Article

   Walchli et al Eur J Immunol. 2014 Mar;44(3):774-84. do

   Insert

   HLA.A2 (HLA-A Human)
   

   Use

   Gateway entry vector

   Tags

   EGFP

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Ypet-PBD

   Plasmid

   #105290

   Purpose

   To measure Rac or Cdc42 activity by binding and FRET analysis

   Depositor

   Kenneth Yamada

   Article

   Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

   Insert

   Pak1 (PAK1 Human)
   

   Tags

   Ypet

   Expression

   Mammalian

   Mutation

   aa encoding 65-150

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-SlugABE-NNG-P2A-puro

   Plasmid

   #214354

   Purpose

   Expresses SlugABE-NNG and puromycin resistance genes

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SlugABE-NNG and puromycin resistance genes
   

   Expression

   Mammalian

   Mutation

   Q782R/S888R/L906R/N984S/E1012K/K1016I

   Available Since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Nanog-2A-mCherry

   Plasmid

   #59995

   Purpose

   Inserts mCherry immediately downstream of the Nanog coding region

   Depositor

   Rudolf Jaenisch

   Article

   Faddah et al Cell Stem Cell. 2013 Jul 3;13(1):23-9. d

   Insert

   Nanog (Nanog Mouse)
   

   Use

   Mouse Targeting

   Tags

   2A-mCherry

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-SlugCas9-NNG-P2A-puro

   Plasmid

   #214352

   Purpose

   Expresses SlugCas9-NNG and puromycin resistance genes

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SlugCas9-NNG and puromycin resistance genes
   

   Expression

   Mammalian

   Mutation

   Q782R/S888R/L906R/N984S/E1012K/K1016I

   Available Since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQE81L-KOFP7

   Plasmid

   #160330

   Purpose

   This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is comparable to EGFP.

   Depositor

   Sang Taek Jung

   Article

   Ko et al J Agric Food Chem. 2020 May 27;68(21):58

   Insert

   KOFP7
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcpBpaRS

   Plasmid

   #196484

   Purpose

   To express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.

   Depositor

   Kensaku Sakamoto

   Article

   Hino et al Methods Mol Biol. 2012;794:215-28. doi:

   Insert

   benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
   

   Expression

   Mammalian

   Promoter

   CMV (synthetase), Pol III tRNA promoter (tRNA)

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDAS12230_pegRNA-PEAR-GFP(10PBS-24RT)-mCherry

   Plasmid

   #177182

   Purpose

   plasmid expressing a pegRNA targeting the PEAR-GFP plasmid along with an mCherry marker

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Insert

   pegRNA targeting the PEAR-GFP plasmid
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP HDAC5 WT

    Plasmid

    #32211

    Depositor

    Reuben Shaw

    Article

    Mihaylova et al Cell. 2011 May 13;145(4):607-21.

    Insert

    HDAC5 (HDAC5 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Nov. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-U6sgp53-U6sgbbSapI-GFAPCre

    Plasmid

    #100275

    Purpose

    AAV-CRISPR mediated genome editing in glia / astrocytes

    Depositor

    Sidi Chen

    Article

    Chow et al Nat Neurosci. 2017 Aug 14. doi: 10.1038/

    Type

    Empty backbone

    Use

    AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…

    Expression

    Mammalian

    Available Since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR_PGK_SNIPR_ALPPL2 Hinge Notch SNIPR

    Plasmid

    #188377

    Purpose

    Lentiviral vector - constitutive expression of an anti-ALPPL2 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiALPPL2_CD8alpha-variant2-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
    

    Use

    Lentiviral

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Nanog-2A-GFP

    Plasmid

    #59994

    Purpose

    Inserts GFP immediately downstream of the Nanog coding region

    Depositor

    Rudolf Jaenisch

    Article

    Faddah et al Cell Stem Cell. 2013 Jul 3;13(1):23-9. d

    Insert

    Nanog (Nanog Mouse)
    

    Use

    Mouse Targeting

    Tags

    2A-GFP

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMS_366

    Plasmid

    #182131

    Purpose

    expresses retron RNA, ec.86 Reverse Transcriptase, and CspRecT SSAP, to create a specific rifampicin resistance mutation

    Depositor

    George Church

    Article

    Schubert et al Proc Natl Acad Sci U S A. 2021 May 4;118

    Inserts

    retron RNA
    ec.86 RT
    CspRecT
    

    Mutation

    incorporated rifampicin-resistance-conferring don…

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-parkin

    Plasmid

    #227367

    Purpose

    For bacterial expression of parkin

    Depositor

    Kalle Gehring

    Article

    Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

    Insert

    Parkin (PRKN Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression.

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. mApple-Annexin(A4)-12

    Plasmid

    #54867

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Annexin (A4) (ANXA4 Human)
    

    Tags

    mApple

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pFRT-PonA-BI-Gl-WT-24xMS2-PTC-18xPP7 (WP)

    Plasmid

    #189792

    Purpose

    Express wild-type and PTC-containing beta-globin genes bi-directionally under ponasteron A inducible promoter in mammalian cells

    Depositor

    Robert Singer

    Article

    Sato et al Nat Commun. 2021 Dec 10;12(1):7203. doi:

    Insert

    beta-globin (HBB Human, Mouse)
    

    Expression

    Mammalian

    Promoter

    Ponasterone A inducible

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEY41

    Plasmid

    #191045

    Purpose

    dat-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    dat-1 (dat-1 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDNR-SpCas9

    Plasmid

    #80425

    Purpose

    Used for in vitro transcription of SpCas9 mRNA.

    Depositor

    Sara Howden, Melissa Little

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    none

    Mutation

    codon optimized for human

    Promoter

    T7

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. AZ64_pE.DonorCLYBL.TS

    Plasmid

    #199228

    Purpose

    Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

    Insert

    Expression unit for EGFP reporter
    

    Use

    Gene targeting donor plasmid

    Expression

    Mammalian

    Promoter

    Human PGK1 gene promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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