We narrowed to 10,362 results for: Coli

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C514-E54: attB5r-RBS-His6-Halotag-nostop-attB1r

Plasmid

#162965

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal His6-Halotag-tagged fusion proteins in bacterial expression

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-Halotag fusion tag with upstream E. coli ribosome binding site

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E51: attB5r-RBS-His6-MBP-nostop-attB1r

Plasmid

#162964

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal His6-MBP-tagged fusion proteins in bacterial expression

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-Maltose-binding protein fusion tag with upstream E. coli ribosome binding site

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-tetpA(R1R4)-iRPT

Plasmid

#154882

Purpose

Ubiquitously expresses iRFP713, Puromycin resistance gene, and rtTAM2.

Depositor

Hideyuki Okano

Article

Nemoto et al Cells. 2020 Dec 22;10(1). pii: cells1001

Inserts

iRFP713
Puromycin resistance gene
rtTAM2

Expression

Mammalian

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-Avi-Coh (E154C)-HIS

Plasmid

#153447

Purpose

E. coli expression of Rc. CohE construct with E154C mutation.

Depositor

Michael Nash

Article

Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

Insert

Rc.CohE

Tags

6xHis and Avi-tag

Expression

Bacterial

Mutation

E154C mutation on CohE

Promoter

T7 promoter

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1-tglA(C51)HIB

Plasmid

#134675

Purpose

Expresses His6-TglA(C51), TglH, TglI and TglB in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Inserts

TglA(C51)
TglH
TglI
TglB

Tags

His6

Expression

Bacterial

Mutation

insertion of cysteine 51

Promoter

T7

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pACYCDuet1-ammA(∆50-56)

Plasmid

#134684

Purpose

Expresses His6-AmmA(∆50-56) in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

ammA(del50-56)

Tags

His6

Expression

Bacterial

Mutation

deletion of amino acids 50-56

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pHis8-4b-McDAH

Plasmid

#140129

Purpose

Expresses McDAH with N-terminal 8xHis-tag in BL21 E. coli

Depositor

Jing-Ke Weng

Article

Kim et al Nat Commun. 2020 Apr 20;11(1):1867. doi:

Insert

Dechloroacutumine halogenase from Menispermum canadense

Tags

8x-His, TEV protease site (ENLYFQ)

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pSB4C5_J23101-vdh

Plasmid

#128130

Purpose

Expressing vdh gene (for the enzyme transforming benzaldehyde to benzoate) under control of the constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

vdh

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23101

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 mouse Tankyrase1 ARC2-3 (308-655)

Plasmid

#132637

Purpose

expresses mouse Tankyrase1 ARC2-3 (308-655) in E. coli

Depositor

Wenqing Xu

Article

Morrone et al Proc Natl Acad Sci U S A. 2012 Jan 31;10

Insert

Tankyrase1 (308-655)

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pBEAST_J23101-HipO

Plasmid

#128133

Purpose

The cell-free adapted backbone, pBEAST, expressing HipO (the enzyme converting hippurate to benzoate) under control of the constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

HipO

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23101

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBEAST_J23101-vdh

Plasmid

#128134

Purpose

The cell-free adapted backbone, pBEAST, expressing vdh (the enzyme converting benzaldehyde to benzoate) under control of the constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

vdh

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23101

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pMH-HT_SsCHAD-H29A-R32A-R36A

Plasmid

#127786

Purpose

Expresses a SsCHAD mutant protein in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

SsCHAD (SULM_RS11045 Sulfolobus solfataricus)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

mutations in His29, Arg32 and Arg36 to Ala

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMH-HT_CtCHAD-R438A-K441A

Plasmid

#127791

Purpose

Expresses a CtCHAD mutant protein in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

CtCHAD (CT0884 Chlorobium tepidum)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Residues 208-522 (CHAD domain), mutations in Arg4…

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI177

Plasmid

#126077

Purpose

Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP1-10 and expression in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

ccdB Cassette (BsaI)
GFP1-10

Use

Binary, module 1

Tags

linker (GTGGGGS)

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI129

Plasmid

#126210

Purpose

Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11x7 and expression in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

ccdB Cassette (BsaI)
GFP11x7 (5 aa linker)

Use

Binary, module 1

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI161

Plasmid

#126211

Purpose

Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11 and expression in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

ccdB Cassette (BsaI)
GFP11

Use

Binary, module 1

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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BPK2013-BsCas12b

Plasmid

#121952

Purpose

E. coli expression, protein purification

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

BsCas12b

Tags

10xHis tag and 3xHA tag

Expression

Bacterial

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pET15b+R2(L171C)

Plasmid

#108239

Purpose

Expresses PdR-PCNA2(L171C) fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

PdR-PCNA2(L171C) fusion protein

Tags

His6 tag

Expression

Bacterial

Mutation

PCNA2,L171C

Promoter

T7 promoter

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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CTL2 (M2225R)

Plasmid

#96952

Purpose

Expresses C-Terminal segment of C. elegans Piezo1 with M2225R mutation in E. coli

Depositor

Douglas Rees

Article

Kamajaya et al Structure. 2014 Oct 7;22(10):1520-7. doi

Insert

CTL2 (M222R) of PIEZO1

Tags

6x Histidine Tag

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pRSM13

Plasmid

#101644

Purpose

Encodes the fluorescent protein meleCFP for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

meleCFP

Tags

6XHis

Expression

Bacterial

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a - TryX

Plasmid

#89623

Purpose

[Edit] Expresses recombinant 6HIS tagged T. cruzi tryparedoxin protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Tryparedoxin

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pPD3cC

Plasmid

#86972

Purpose

Expresses PCNA3 variant (R112C/T180C) fused phosphite dehydrogenase and P450cam in E. coli

Depositor

Teruyuki Nagamune

Article

Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

Insert

PCNA3 variant (R112C/T180C) fused to phosphite dehydrogenase and P450cam

Tags

His6 tag

Expression

Bacterial

Mutation

E175A/A176R in PTDH, N106R/R112C/T180C in PCNA3, …

Promoter

T7 promoter

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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p1G108CP20X

Plasmid

#85096

Purpose

Expresses PCNA1 variant (G108C) fused to putidaredoxin in E. coli

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

The G108C variant of PCNA1 fused to putidaredoxin

Tags

His6 tag

Expression

Bacterial

Mutation

G108C in PCNA1

Promoter

T7 promoter

Available Since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pRSETb-pcDronpa

Plasmid

#78183

Purpose

Expresses pcDronpa in E. coli strains

Depositor

Peter Dedecker

Article

Moeyaert et al ACS Nano. 2014 Feb 25;8(2):1664-73. doi:

Insert

pcDronpa

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pJK278

Plasmid

#71134

Purpose

Expression of Acetobacter aceti 1023 PurE (H89N mutant) in E. coli

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-89 to asparagine; additional N-…

Promoter

T7

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pJK130

Plasmid

#71129

Purpose

Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide synthetase (PurK) in E. coli

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide synthetase

Tags

His6

Expression

Bacterial

Mutation

changed valine-319 to phenylalanine

Promoter

T7

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pJK320

Plasmid

#71144

Purpose

Expression of Acetobacter aceti 1023 PurE (H59S mutant) in E. coli

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-59 to serine; additional N-term…

Promoter

T7

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pJK319

Plasmid

#71143

Purpose

Expression of Acetobacter aceti 1023 PurE (H59A mutant) in E. coli

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-59 to alanine; additional N-ter…

Promoter

T7

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK318

Plasmid

#71142

Purpose

Expression of Acetobacter aceti 1023 PurE (H59Q mutant) in E. coli

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-59 to glutamine; additional N-t…

Promoter

T7

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pJK315

Plasmid

#71141

Purpose

Expression of Acetobacter aceti 1023 PurE (H89G mutant) in E. coli

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-89 to glycine; additional N-ter…

Promoter

T7

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK314

Plasmid

#71140

Purpose

Expression of Acetobacter aceti 1023 PurE (H89V mutant) in E. coli

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-89 to valine; additional N-term…

Promoter

T7

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK279

Plasmid

#71135

Purpose

Expression of Acetobacter aceti 1023 PurE (H89D mutant) in E. coli

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-89 to aspartate; additional N-t…

Promoter

T7

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK283

Plasmid

#71139

Purpose

Expression of Acetobacter aceti 1023 PurE (H59N mutant) in E. coli

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-59 to asparagine; additional N-…

Promoter

T7

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK282

Plasmid

#71138

Purpose

Expression of Acetobacter aceti 1023 PurE (H59D mutant) in E. coli

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-59 to aspartate; additional N-t…

Promoter

T7

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK281

Plasmid

#71137

Purpose

Expression of Acetobacter aceti 1023 PurE (H59F mutant) in E. coli

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-59 to phenylalanine; additional…

Promoter

T7

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK280

Plasmid

#71136

Purpose

Expression of Acetobacter aceti 1023 PurE (H89F mutant) in E. coli

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-89 to phenylalanine; additional…

Promoter

T7

Available Since

Jan. 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST14-ARL4C

Plasmid

#67381

Purpose

Expression of native hArl4c in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL7 (ARL4C Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-ARL8A

Plasmid

#67382

Purpose

Expression of native hArl8 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8A (ARL8A Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-ARF3

Plasmid

#67370

Purpose

Expression of native hArf3 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF3 (ARF3 Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-ARF4

Plasmid

#67371

Purpose

Expression of native hArf4 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF4 (ARF4 Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

Showing: 7321 - 7360 of 10362 results

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