We narrowed to 14,304 results for: TIM;

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pYZ172

Plasmid

#98409

Purpose

Donor DNA plasmid to introduce lys9 deletion in S. pombe. Linearization with Not1 digestion.

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Inserts

upstream homologous region to delete lys9 in genome by recombination
downstream homologous region to delete lys9 in genome by recombination

Use

CRISPR and Synthetic Biology

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pYSD300

Plasmid

#212930

Purpose

Encodes the S. cerevisiae Aga1 protein (Part3) with pGBK1 promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS yeast origin.

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

AGA1 (AGA1 Budding Yeast)

Expression

Yeast

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pXNgate22-1HA

Plasmid

#105077

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

Depositor

Wolf Frommer

Article

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Tags

NubG

Expression

Yeast

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pSws1-nfsB-CFP

Plasmid

#86375

Purpose

for zebrafish transgenesis, expression of nfsB-CFP, also expresses EGFP

Depositor

Rachel Wong

Article

Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

Insert

nitroreductase (nfsB bacteria)

Use

Zebrafish

Tags

ECFP

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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PKG I alpha dNPS

Plasmid

#16391

Depositor

Bernard Weinstein

Article

Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

Insert

PKG1 alpha (PRKG1 Human)

Tags

HA

Expression

Mammalian

Mutation

N-terminal pseudosubstrate domain deletion: delet…

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
roGFP_iE_C147S_pQE30

Plasmid

#48631

Purpose

Bacterial expression vector encoding for the 6XHis tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 147 to Serine mutation (roGFP C147S, E147b, C204)

Depositor

David Ron

Article

Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

Insert

roGFP1

Tags

HisTag

Expression

Bacterial

Mutation

insertion E147B, C147S

Available Since

Oct. 8, 2013

Availability

Academic Institutions and Nonprofits only

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pCR3.1 GSK3beta S9A HA

Plasmid

#15994

Depositor

Morris Birnbaum

Article

Summers et al J Biol Chem. 1999 Jun 18. 274(25):17934-

Insert

GSK3beta (GSK3B Human)

Tags

HA

Expression

Mammalian

Mutation

S9A: constitutively active

Available Since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA flag-Pcbp2

Plasmid

#184028

Purpose

Codon optimized Pcbp2 clone with N-terminal flag tag

Depositor

Peter Stoilov

Article

Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

Insert

Pcbp2 (Pcbp2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Codon optimized

Promoter

CMV

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pGTag-P2A-QFGal4-SV40pA

Plasmid

#155127

Purpose

Donor for precise CRISPR directed genomic integration of QFGal4 using the GeneWeld method

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

P2A-QFGal4

Use

CRISPR and Synthetic Biology

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiEKAR2G1

Plasmid

#40177

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Use

Lentiviral

Tags

His-Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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1456_pDEST_miniTol2_R4-R2_Cryst-eGFP

Plasmid

#171795

Purpose

pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a eGFP selection marker (Crystallin promoter Lens/Eyes)

Depositor

Jean Giacomotto

Article

Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pFUGW-ZipACR-EYFP

Plasmid

#88844

Purpose

Fast anion channelrhodopsin (current half-decay time 2-4 ms depending on voltage) for time-resolved inhibition of neuronal spiking

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

ZipACR-NotI-EYFP

Use

Lentiviral

Tags

EYFP

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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RT3GEN

Plasmid

#111165

Purpose

Tet-regulated miR-E (miR-30 variant)-based RNAi with tGFP reporter

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Insert

miR-E (miR-30 variant)

Use

Retroviral

Mutation

WT

Promoter

T3G

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-H2B-mRuby3-IRES-eGtACR1-ST

Plasmid

#108960

Purpose

Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

eGtACR1-ST

Expression

Mammalian

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pET-DUET-GST-eIF4G1-His6, eIF4E

Plasmid

#37232

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Inserts

TIF4631 (TIF4631 Budding Yeast)
CDC33 (CDC33 Budding Yeast)

Tags

GST and His6

Expression

Bacterial

Promoter

T7

Available Since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA5/FRT/TO_H2B_HaloTag9

Plasmid

#169334

Purpose

Nuclear expression of HaloTag9 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag9 (H2BC21 Human)

Tags

HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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(326) pAAV SV40-ZsGreen U6-gRNA

Plasmid

#163020

Purpose

Fluorescent reporter for Sa gRNA (Bsa1 sites)

Depositor

Tonia Rex

Article

Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

Insert

Zs Green

Use

AAV

Expression

Mammalian

Promoter

SV40

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pNXgate32-3HA

Plasmid

#105076

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

Depositor

Wolf Frommer

Article

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Tags

3xHA and NubG

Expression

Yeast

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-ING2

Plasmid

#109554

Purpose

Multisite gateway vector for 3' tagging with 3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised. Binds PtdIns5P

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV5 Flag-Smad2 EPSM

Plasmid

#14933

Depositor

Joan Massague

Article

Kretzschmar et al Genes Dev. 1999 Apr 1. 13(7):804-16.

Insert

Smad2 EPSM (SMAD2 Human)

Tags

Flag

Expression

Mammalian

Mutation

Erk/Pro-directed kinase site mutant (EPSM). T220V…

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only