We narrowed to 3,867 results for: mak

1. pCAG-MBP-Foldon-ATG9 (723-839)

   Plasmid

   #188088

   Purpose

   To make ATG9 C-terminal tail trimer for expression in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG9 (ATG9A Human)
   

   Tags

   MBP tag

   Expression

   Mammalian

   Mutation

   ATG9 (723-839)

   Promoter

   CMV

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-MBP-ATG9 (801-839)

   Plasmid

   #188093

   Purpose

   To make monomeric ATG9 C-terminal tail for expression in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG9 (ATG9A Human)
   

   Tags

   MBP tag

   Expression

   Mammalian

   Mutation

   ATG9 (801-839)

   Promoter

   CMV

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-MBP-Foldon-ATG9 (828-839)

   Plasmid

   #188090

   Purpose

   To make ATG9 C-terminal tail trimer for expression in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG9 (ATG9A Human)
   

   Tags

   MBP tag

   Expression

   Mammalian

   Mutation

   ATG9 (828-839)

   Promoter

   CMV

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFlare9A-CD45i4e5i5mutCBS

   Plasmid

   #131835

   Purpose

   A reporter construct containing the Exon 5 of PTPRC (CD45), which contains a mutant CTCF binding site, making the exon to be always excluded.

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   PTPRC (PTPRC Human)
   

   Expression

   Mammalian

   Mutation

   partial intron 4 full exon 5 and partial intron 5…

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTBL405 CHYRON1 integration construct

   Plasmid

   #126442

   Purpose

   To integrate the CHYRON1 locus at HEK293site3.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Inserts

   pU6-CHYRON1 hgRNA
   pCMV-puro
   

   Expression

   Mammalian

   Mutation

   The SpCas9 sgRNA constant region is mutated to ma…

   Promoter

   CMV and human U6

   Available Since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. PCRII apoBa

   Plasmid

   #121897

   Purpose

   Plasmid for making zebrafish apoBa in situ probe

   Depositor

   Steven Farber

   Article

   Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

   Insert

   ApoBa (apoba Zebrafish)
   

   Use

   Unspecified

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV-PIG-N3FLAG-TAF12(50-130)

   Plasmid

   #105595

   Purpose

   retrovirally express mouse TAF12 HFD with 3*FLAG tag at N terminal, GFP marker and puro resistance

   Depositor

   Christopher Vakoc

   Article

   Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

   Insert

   TAF12 (AA 50-130)- histone fold domain-HFD (Taf12 Mouse)
   

   Use

   Retroviral

   Tags

   3*FLAG

   Expression

   Mammalian

   Mutation

   truncation fragments containing aa (50-130), and …

   Promoter

   MSCV-LTR

   Available Since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pB-CAGGS-dCas9-KRAB-MeCP2

   Plasmid

   #110824

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2

   Depositor

   Alejandro Chavez, George Church

   Article

   Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

   Insert

   dCas9-KRAB-MeCP2
   

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CAGGS

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCerulean3-Bcl-W-FUGW

   Plasmid

   #166751

   Purpose

   Express mCerulean3 fused to Bcl-2 family protein, Bcl-W, in a plasmid compatable for lentiviral infection. Used to make stable BMK-DKO cell lines.

   Depositor

   David Andrews

   Article

   Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

   Insert

   Bcl-W (Bcl2l2 Mouse)
   

   Use

   Lentiviral

   Tags

   mCerulean3

   Promoter

   human ubiquitin C

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. BCR/ABL P190 transgenic construct

    Plasmid

    #38185

    Depositor

    Nora Heisterkamp

    Article

    Heisterkamp et al Nature. 1990 Mar 15;344(6263):251-3.

    Insert

    BCR/ABL P190 (ABL1, BCR Human)
    

    Use

    Construct for making transgenic mice

    Promoter

    truncated mouse MT promoter

    Available Since

    Oct. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKIF1C-GFP

    Plasmid

    #130977

    Purpose

    Expression of KIF1C-GFP in mammalian cells.

    Depositor

    Anne Straube

    Article

    Efimova et al J Cell Sci. 2014 Dec 15;127(Pt 24):5179-

    Insert

    KIF1C-GFP (KIF1C Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    5 silent mutations that make this construct RNAi …

    Promoter

    CMV

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pegRNA-GFP>BFP_Y66H-NGG

    Plasmid

    #185480

    Purpose

    pegRNA plasmid in order to make a Y66H (TAC>CAT) conversion in the GFP gene, creating a BFP gene.

    Depositor

    Sabine Fuchs

    Article

    Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

    Insert

    GFP>BFP_Y66H-pegRNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. DENV4 Rluc Reporter GND mutant Replicon

    Plasmid

    #118586

    Purpose

    For use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.

    Depositor

    Radhakrishnan Padmanabhan

    Article

    Alcaraz-Estrada et al J Gen Virol. 2010 Nov;91(Pt 11):2713-8.

    Insert

    DENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
    

    Use

    Yeast-e. col shuttle vector prs424

    Mutation

    The conserved GDD in the NS5 polymerase gene muta…

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pD649-HAsp-SEMA3E-COMP5AP-AviTag-9xHis

    Plasmid

    #157534

    Purpose

    Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    SEMA3E (SEMA3E Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAGGS-RaichuEV-Rac1(Y40C)

    Plasmid

    #164903

    Purpose

    Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation Y40C in the Rac1 domain. More useful than T17N mutation due to less harmful to endogenous signaling.

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    RaichuEV-Rac1 (RAC1 Human)
    

    Expression

    Mammalian

    Mutation

    Y40C mutation in the Rac1 domain whcih make the s…

    Available Since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCerulean3-Bcl-XL-s2193

    Plasmid

    #166749

    Purpose

    Express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-XL, in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines.

    Depositor

    David Andrews

    Article

    Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

    Insert

    Bcl-XL (BCL2L1 Human)
    

    Tags

    mCerulean3

    Expression

    Mammalian

    Promoter

    Human ferritin

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pICE-HA-CtIP-siR-N289A-H290A

    Plasmid

    #82032

    Purpose

    Plasmid for constitutive or doxy-inducible expression of N289A+H290A mutant of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Patrick Calsou, Sébastien Britton

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    CtIP (RBBP8 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Endonuclease-dead mutant: N289A+H290A CtIP; Mutat…

    Promoter

    CMV-tet

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pD649-HAsp-SEMA3F-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156977

    Purpose

    Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    SEMA3F (SEMA3F Human)
    

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pICE-HA-CtIP-siR-S664A-S679A-S745A

    Plasmid

    #82031

    Purpose

    Plasmid for constitutive or doxy-inducible expression of S664A+S679A+S745A human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Patrick Calsou, Sébastien Britton

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    CtIP (RBBP8 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Non phosphorylatable mutations on 3 of the main A…

    Promoter

    CMV-tet

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. HOXB6 (human) FLAG pMSCV

    Plasmid

    #8534

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB6 (HOXB6 Human)
    

    Use

    Retroviral

    Tags

    FLAG and IRES-GFP

    Expression

    Mammalian

    Mutation

    contains improved ribosomal binding site and FLAG…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only