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pMALp2x-SCO5461(43-204)

Plasmid

#42509

Depositor

Koji Okamoto

Article

Nakano et al Toxicon. 2012 Dec 2;63C:55-63. doi: 10.1

Insert

guanosine ADP-ribosyltransferase (SCO5461 Streptomyces coelicolor A3(2))

Tags

maltose-binding protein

Expression

Bacterial

Mutation

deleted amino acids 1-42 (nucleotide 1-126)

Promoter

tac

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only
pDSHA-ARL8A-Myc

Plasmid

#67361

Purpose

Mammalian expression of hArl8 with C-term Myc tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8A (ARL8A Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDSHA-ARF5-Myc

Plasmid

#67351

Purpose

Mammalian expression of hArf5 with C-term Myc tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF5 (ARF5 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARF6

Plasmid

#67314

Purpose

hArf6 with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF6 (ARF6 Human)

Use

Gateway entry vector

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pNHT7::RPL15::sNluc2

Plasmid

#186763

Purpose

An improved mRNA product vector encoding a codon-optimized nanoluciferase (sNluc2) with RPL15 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Promoter

T7

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pWZL Blast H-Ras G12V E37G

Plasmid

#12277

Depositor

Bob Weinberg

Article

Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

Insert

H-Ras G12V E37G (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

Contains G12V activating mutation. The E37G mutat…

Available Since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only
pWZL Blast H-Ras G12V Y40C

Plasmid

#12279

Depositor

Bob Weinberg

Article

Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

Insert

H-Ras G12V Y40C (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

Contains G12V activating mutation. The Y40C mutat…

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only
CBP-Bromo-HAT-CH3-sp65

Plasmid

#21026

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

CBP (Crebbp Mouse)

Use

In vitro tnt

Tags

Flag

Mutation

Bromo+HAT+CH3 domains aa 1098-1877; E1239G mutati…

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pBV-Luc/Del-7

Plasmid

#14968

Depositor

Joan Massague

Article

Chen et al Proc Natl Acad Sci U S A. 2001 Jan 30. 9

Insert

c-myc promoter (-26/+334) (MYC Human)

Use

Luciferase

Expression

Mammalian

Mutation

-26/+334 (relative to the P2 transcription initia…

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pNTPTP alpha (S180A/S204A)

Plasmid

#17700

Depositor

Xinmin Zheng

Article

Zheng et al J Biol Chem. 2002 Jun 14. 277(24):21922-

Insert

PTPalpha S180A S204A (PTPRA Human)

Expression

Mammalian

Mutation

S180A / S204A

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pLKO-shcIAP1-B

Plasmid

#44131

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

c-IAP1 (BIRC2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only
gh178

Plasmid

#106828

Purpose

expression of gRNA targeting GYS1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GYS1 (GYS1 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pBV-Luc/Frag-F

Plasmid

#14053

Depositor

Joan Massague

Article

Chen et al Proc Natl Acad Sci U S A. 2001 Jan 30. 9

Insert

c-myc promoter (-109/-3) (MYC Human)

Use

Luciferase

Expression

Mammalian

Mutation

-109/-3 (relative to the P2 transcription initiat…

Available Since

May 10, 2007

Availability

Academic Institutions and Nonprofits only
pQCXIpuro-H3.3K4M-FLAG/HA

Plasmid

#128742

Purpose

Expression of histone H3.3K4M-FLAG/HA

Depositor

Kai Ge

Article

Jang et al Nucleic Acids Res. 2019 Jan 25;47(2):607

Insert

H3.3K4M (H3-3A Human)

Use

Retroviral

Tags

FLAG and HA

Mutation

K4M

Promoter

CMV

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD-SCW-HHL

Plasmid

#190627

Purpose

Bacterial expression of genetically encoded calcium indicator SCW-HHL

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

SCW-HHL

Expression

Bacterial

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-SPW-HHL

Plasmid

#190629

Purpose

Bacterial expression of genetically encoded protease indicator SPW-HHL

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

SPW-HHL

Expression

Bacterial

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pONSY-Lifeact:mCherry

Plasmid

#111876

Purpose

This plasmid expresses mCherry fused to Lifeact peptide. It labels actin bundles and filopodia in Capsaspora. Codons have been optimised according to their usages in Capsaspora and C. fragrantissima.

Depositor

Inaki Ruiz-Trillo

Article

Parra-Acero et al Development. 2018 May 11. pii: dev.16210

Insert

Lifeact fused to mCherry

Use

Capsaspora owczarzaki

Tags

mCherry

Promoter

Elongation Factor 1 alpha (EF1a) from Capsaspora

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST17-6xHis-ARL2

Plasmid

#67474

Purpose

Expression of hArl2 with N-term His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL2 (ARL2 Human)

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARF4

Plasmid

#67455

Purpose

Mammalian expression of hArf4 with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF4 (ARF4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARF3

Plasmid

#67308

Purpose

hArf3 with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF3 (ARF3 Human)

Use

Gateway entry vector

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only