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HLA_DRA_01_01

Plasmid

#165012

Purpose

Expression of HLA-DRA*01:01 cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

HLA-DRA (HLA-DRA Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCMU-MITr

Plasmid

#61198

Purpose

Fluorescent reporter for mitochondria ScCOX4sp-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

ScCOX4sp-mCherry

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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pDIS3

Plasmid

#92173

Purpose

Vector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by disruption of the region targeted for recombination.

Depositor

Judith Berman

Article

Gerami-Nejad et al Microbiology. 2013 Mar;159(Pt 3):565-79.

Insert

NAT1

Use

Genbank: u03440.1

Expression

Yeast

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pDisplay-FRISZ

Plasmid

#176887

Purpose

Far-red fluorescent zinc indicator FRISZ in pDisplay

Depositor

Huiwang Ai

Article

Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

Insert

FRISZ

Tags

pDisplay backbone elements for export and membran…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_GEM2-Halo-FRB

Plasmid

#197056

Purpose

Expression of Synechococcus elongatus encapsulin Halo-FRB-fusion in mammalian cells

Depositor

Julia Mahamid

Article

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Insert

Encapsulin

Tags

FRB and HaloTag

Expression

Mammalian

Mutation

Codon-optimised for human cell expression

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pDAS12395_PEAR-GFP_2.0-preedited

Plasmid

#177185

Purpose

PEAR-GFP3 control plasmid with split EGFP and intact 5' splice site

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDAS12125_PEAR-GFP

Plasmid

#177178

Purpose

PEAR-GFP target plasmid with split EGFP and disrupted 5' splice site

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Expression

Mammalian

Mutation

disrupted 5' splice site

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-P-RAN-GFP41

Plasmid

#106407

Purpose

P-RAN-GFP41 (RANbody GFP41::vHRP)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

P-RAN-GFP41

Tags

HA and His

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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lenti-Cas9-VQR-Blast

Plasmid

#87155

Purpose

lentiviral expression of SpCas9-VQR (NGA PAM restricted)

Depositor

Daniel Bauer

Article

Canver et al Nat Genet. 2017 Feb 20. doi: 10.1038/ng.

Insert

SpCas9-VQR(D1135V,R1335Q,T1337R)

Use

Lentiviral

Mutation

D1135V,R1335Q,T1337R

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-myc-SNAP-GP135

Plasmid

#50376

Purpose

Express myc and SNAP-tagged GP135 in mammmalian cells

Depositor

Reika Watanabe

Article

Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

Insert

SNAP

Expression

Mammalian

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
Ndel1 RNAi

Plasmid

#12573

Depositor

Li-Huei Tsai

Article

Shu et al Neuron. 2004 Oct 14. 44(2):263-77.

Insert

Ndel1 shRNA (NDEL1 Human)

Use

RNAi

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA-NES-ZapCY2

Plasmid

#59016

Purpose

Cytosolic zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Qin et al ACS Chem Biol. 2013 Nov 15;8(11):2366-71

Insert

NES-ZapCY2 genetically encoded cytosolic Zn(II) sensor

Tags

Nuclear Exclusion Signal

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_rLP5(nth-ydgR)

Bacterial Strain

#110245

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the nth-ydgR intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
pLenti_ABE8e-SpRY-P2A-BFP_HygroR

Plasmid

#213009

Purpose

A lentiviral vector used to create stable cell lines expressing the ABE8e-SpRY base editor and BFP

Depositor

Richard Sherwood

Article

Ryu et al Nat Genet. 2024 Apr 24. doi: 10.1038/s41

Insert

ABE8e-SpRY-D10A

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A nickase variant of SpRY

Promoter

Ef-1a

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-mitoEcSTH-FLAG

Plasmid

#218629

Purpose

This plasmid contains human codon optimized sequence of mitoEcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.

Depositor

Valentin Cracan

Article

Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

Insert

mitoEcSTH

Use

Lentiviral and Synthetic Biology

Tags

FLAG and MTS

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pS148∙CsR

Plasmid

#197858

Purpose

Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

gRNA scaffold - Ap/Cb resistance cassette - incP oriT

Use

CRISPR and Synthetic Biology

Promoter

PEM7

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-Ki67-mCherry

Plasmid

#183748

Purpose

Expresses EGFP-Ki67-mCherry

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67 (MKI67 Human)

Tags

Ki-67 with N-terminal EGFP tag and Ki-67 with C-…

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAtetO_6xHis-v5.9

Plasmid

#155259

Purpose

Plasmid for the aTc-inducible expression of the v5.9

Depositor

Andrew Ellington

Article

Milligan et al Biochemistry. 2018 Aug 7;57(31):4607-461

Insert

v5.9

Tags

6xHis

Expression

Bacterial

Promoter

tet PA

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pAG244 iFAST

Plasmid

#130809

Purpose

Expresses iFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Biochemistry. 2018 Oct 2;57(39):5648-565

Insert

iFAST

Tags

His-tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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LZF97_hSyn-spine-jRGECO1a

Plasmid

#119198

Purpose

Targets jRGECO1a to spines in living neurons.

Depositor

Don Arnold

Article

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

jRGECO1a-TPR3-Utrophin(1-78)-TPR3-Zfn(CCR5-L) (Utrn Synthetic, Rat)

Use

Lentiviral

Expression

Mammalian

Promoter

Human Synapsin 1

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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