We narrowed to 11,256 results for: NSI

1. Nsp14D-EGFP

   Plasmid

   #165125

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Nsp14D (ORF1ab codon optimized SARS-COV-2)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGL3-FLIP pr4

   Plasmid

   #19129

   Depositor

   Wafik El-Deiry

   Article

   Ricci et al Mol Cell Biol. 2004 Oct . 24(19):8541-55

   Insert

   FLIP promoter (CFLAR Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   The sequence corresponds to positions -503 to +10…

   Promoter

   Available since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pDEST40-2XHA-Src-S32-KD

   Plasmid

   #140310

   Purpose

   Expresses HA-tagged human Src-S32-KD in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src deletion mutant (SRC Human)
   

   Use

   Tags

   2X HA

   Expression

   Mammalian

   Mutation

   Contains only the SH3, SH2, and KD regions

   Promoter

   CMV

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDONR223-GOLPH3

   Plasmid

   #21688

   Depositor

   Lynda Chin

   Article

   Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

   Insert

   Golgi Phosphoprotein 3 (GOLPH3 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   last nucleotide of stop codon removed for c-termi…

   Promoter

   Available since

   July 28, 2009

   Availability

   Academic Institutions and Nonprofits only

5. Epac-S-H96

   Plasmid

   #170342

   Purpose

   CFP(UST)Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.

   Insert

   CFP(UST)Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMTTH+mEGFP-LANA-H1.4CTD

   Plasmid

   #157802

   Purpose

   Expression of mEGFP, LANA peptide, and human linker histone H1.4 CTD fusion protein

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   mEGFP-LANA-H1.4CTD (H1-4 Human)
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   pTAC

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDEST27-GST-ATXN1 FL(30Q)-S776D

   Plasmid

   #21755

   Depositor

   Huda Zoghbi

   Article

   Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

   Insert

   Ataxin-1 (ATXN1 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   insert contains a stretch of 32 amino acids (30 Q…

   Promoter

   CMV

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pHH0103_ITSN1-2/5

   Plasmid

   #91385

   Purpose

   Protein expression and purification of human SH3 domain construct ITSN1-2/5

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   ITSN1-2/5 (ITSN1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pXPR_BRD003 sgATXN1L-1

   Plasmid

   #74970

   Purpose

   sgATXN1L-1, puromycin selection

   Depositor

   William Hahn

   Article

   Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

   Insert

   ATXN1L (ATXN1L Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PX459v2-ILK-gRNA 1-exon 1

    Plasmid

    #163320

    Purpose

    SpCas9 ILK gRNA 1 (G*CGGAGAACGACCTCAACCAG), targeting exon 1 within the N-terminal ankyrin repeat domain-1.

    Depositor

    Val Brunton

    Article

    Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

    Insert

    ILK gRNA 1_Exon1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

11. PX459v2-ILK-gRNA 2-exon 8

    Plasmid

    #163321

    Purpose

    SpCas9 ILK gRNA 2 (G*ACATTGTAGAGGGATCCATA), targeting exon 8 within the C-terminal protein kinase domain.

    Depositor

    Val Brunton

    Article

    Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

    Insert

    ILK gRNA 2_Exon8
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6F

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

12. TVBB C-term-Dendra2

    Plasmid

    #169216

    Purpose

    Targeting vector backbone to support a knock-in of Linker-Dendra2 at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked Dendra2-P2A-Puro
    

    Use

    Targeting vector backbone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHH0103_LASP1-1/1-SV1/2

    Plasmid

    #91399

    Purpose

    Protein expression and purification of human SH3 domain construct LASP1-1/1-SV1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    LASP1-1/1-SV1/2 (LASP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pXPR_BRD003 sgCIC-2

    Plasmid

    #74967

    Purpose

    sgCIC-2, puromycin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    CIC (CIC Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LP725

    Plasmid

    #90182

    Purpose

    pEGFP-C1 with cDNA encoding HsKIF13B aa residues 561-1826

    Depositor

    Lotte Pedersen

    Article

    Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

    Insert

    KIF13B (KIF13B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa residues 561-1826

    Promoter

    CMV

    Available since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LP399

    Plasmid

    #90176

    Purpose

    pEGFP-C1 with cDNA encoding HsKIF13B aa residues 1-1289

    Depositor

    Lotte Pedersen

    Article

    Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

    Insert

    KIF13B (KIF13B Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    aa residues 1-1289

    Promoter

    CMV

    Available since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHH0103_GRB2-2/2

    Plasmid

    #91410

    Purpose

    Protein expression and purification of human SH3 domain construct GRB2-2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    GRB2-2/2 (GRB2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-45b-RUNX2(+10A)-IDR-mCherry

    Plasmid

    #145287

    Purpose

    Bacterial Expression of 6xHis RUNX2(+10A) IDR mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    RUNX2(+10A)-IDR (RUNX2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A-repeat expanded

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHH0103_GRAP2-2/2

    Plasmid

    #91368

    Purpose

    Protein expression and purification of human SH3 domain construct GRAP2-2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    GRAP2-2/2 (GRAP2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. C-His-GvpC-Calpain

    Plasmid

    #153295

    Purpose

    Expresses calpain-sensitive variant of Anabaena flos-aquae GvpC in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    Calpain-sensitive Gas vesicle protein C (his-tagged)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    1) Contains an extra glycine after the methionine…

    Promoter

    T7

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHH0103_CRK-2/2-SV2/2

    Plasmid

    #91343

    Purpose

    Protein expression and purification of human SH3 domain construct CRK-2/2-SV2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    CRK-2/2-SV2/2 (CRK Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHH0103_EPS8L2-1/1

    Plasmid

    #91526

    Purpose

    Protein expression and purification of human SH3 domain construct EPS8L2-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    EPS8L2-1/1 (EPS8L2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1 2xFLAG-2xSTREP EMI1

    Plasmid

    #158947

    Purpose

    Express human EMI1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

    Insert

    EMI1 (FBXO5 Human)
    

    Use

    Tags

    2X FLAG 2X STREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHH0103_SRC-1/1-IS1/2

    Plasmid

    #91349

    Purpose

    Protein expression and purification of human SH3 domain construct SRC-1/1-IS1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SRC-1/1-IS1/2 (SRC Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGAL4-DBD-EOMESwt-IDR

    Plasmid

    #145231

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and EOMESwt IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    EOMESwt-IDR (EOMES Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGAL4-DBD-BHLHE41wt-IDR

    Plasmid

    #145229

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and BHLHE41wt IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    BHLHE41wt-IDR (BHLHE41 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGAL4-DBD-HOXA13wt-IDR

    Plasmid

    #145240

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and HOXA13wt IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXA13wt-IDR (HOXA13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHH0103_ASAP1-1/1

    Plasmid

    #91501

    Purpose

    Protein expression and purification of human SH3 domain construct ASAP1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ASAP1-1/1 (ASAP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGL3-158/+19

    Plasmid

    #35541

    Depositor

    Greg Goodall

    Article

    Bracken et al Cancer Res. 2008 Oct 1;68(19):7846-54.

    Insert

    promoter region of miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    none

    Available since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGL3-110/+19

    Plasmid

    #35542

    Depositor

    Greg Goodall

    Article

    Bracken et al Cancer Res. 2008 Oct 1;68(19):7846-54.

    Insert

    promoter region of miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    none

    Available since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. LP696

    Plasmid

    #90177

    Purpose

    pEGFP-C1 with cDNA encoding HsKIF13B aa residues 1289-1826

    Depositor

    Lotte Pedersen

    Article

    Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

    Insert

    KIF13B (KIF13B Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    aa residues 1289-1826

    Promoter

    CMV

    Available since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PCRII apoEa

    Plasmid

    #121900

    Purpose

    Plasmid for making zebrafish apoEa in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    ApoEa (apoea Zebrafish)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pXPR_BRD003 sgATXN1L-2

    Plasmid

    #74971

    Purpose

    sgATXN1L-2, puromycin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    ATXN1L (ATXN1L Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHH0103_DOCK5-1/1-IS1/2

    Plasmid

    #91303

    Purpose

    Protein expression and purification of human SH3 domain construct DOCK5-1/1-IS1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    DOCK5-1/1-IS1/2 (DOCK5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHH0103_DNMBP-6/6

    Plasmid

    #91489

    Purpose

    Protein expression and purification of human SH3 domain construct DNMBP-6/6

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    DNMBP-6/6 (DNMBP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pHH0103_KALRN-1/4

    Plasmid

    #91283

    Purpose

    Protein expression and purification of human SH3 domain construct KALRN-1/4

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    KALRN-1/4 (KALRN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET15b-ITPA_P32T

    Plasmid

    #60825

    Purpose

    to purify human ITPA from bacteria

    Depositor

    Youri Pavlov

    Article

    Stepchenkova et al J Mol Biol. 2009 Sep 25;392(3):602-13. d

    Insert

    ITPA (ITPA Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    P32T

    Promoter

    T7

    Available since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

38. pHH0103_ITSN1-1/5-IS2/3

    Plasmid

    #91318

    Purpose

    Protein expression and purification of human SH3 domain construct ITSN1-1/5-IS2/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ITSN1-1/5-IS2/3 (ITSN1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pHH0103_LASP1-1/1-SV2/2

    Plasmid

    #91392

    Purpose

    Protein expression and purification of human SH3 domain construct LASP1-1/1-SV2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    LASP1-1/1-SV2/2 (LASP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. LP344

    Plasmid

    #90174

    Purpose

    pEGFP-C1 with cDNA encoding HsKIF13B aa residues 1-370

    Depositor

    Lotte Pedersen

    Article

    Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

    Insert

    KIF13B (KIF13B Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    aa residues 1-370

    Promoter

    CMV

    Available since

    May 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge