We narrowed to 3,867 results for: mak

1. pET28B-PU.1-ETS-Wing

   Plasmid

   #124552

   Purpose

   Chimeric ETS domain: Wing of Ets-1 in PU.1 scaffold

   Depositor

   Gregory Poon

   Article

   Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

   Insert

   PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   The 5 residues making up the "wing" in …

   Available Since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAVS1-TRE-MA4-GFP

   Plasmid

   #114380

   Purpose

   Doxycycline inducible MLL-AF4 and GFP expression. The construct has been used with CAG-rtTA for making engraftable iPSC derived HSCs.

   Depositor

   Yuet Wai Kan

   Article

   Tan et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

   Insert

   MLL-AF4 fusion gene (AFF1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA4/TO/GFP-GIGYF2

   Plasmid

   #141189

   Purpose

   Expresses GFP-GIGYF2 in mammalian cells, can be used to make inducible cell line

   Depositor

   Simon Bekker-Jensen

   Article

   Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

   Insert

   GIGYF2 (GIGYF2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-shFMR1-mCherry

   Plasmid

   #222963

   Purpose

   Make lentivirus to knock down human FMR1 gene

   Depositor

   Xinyu Zhao

   Article

   Guo et al Nat Commun. 2023 Jun 26;14(1):3801. doi:

   Insert

   FMR1 shRNA (FMR1 Human)
   

   Use

   Lentiviral

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pinducer 20 DN-KASHΔPPPL

   Plasmid

   #129280

   Purpose

   Tet-ON inducible lentivirus expressing mcherry-labeled DN KASHΔPPPL

   Depositor

   Daniel Conway

   Article

   Zhang et al Curr Biol. 2019 Aug 2. pii: S0960-9822(1

   Insert

   SYNE1 (SYNE1 Human)
   

   Use

   Lentiviral

   Tags

   mcherry

   Mutation

   the last 4 amino acids (PPPL) of the KASH domain …

   Promoter

   CMV

   Available Since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA4/TO/Strep-HA-ZAK alpha

   Plasmid

   #141193

   Purpose

   Expresses Strep-HA-ZAK alpha in mammalian cells, can be used to make inducible cell line

   Depositor

   Simon Bekker-Jensen

   Article

   Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

   Insert

   MAP3K20 (MAP3K20 Human)
   

   Tags

   Strep, HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSIN-PAmCherry-mSNCA-NE

   Plasmid

   #102364

   Purpose

   Lentiviral overexpression of PA-mCherry synuclein alpha fusion

   Depositor

   Shu Leong Ho

   Article

   Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

   Inserts

   PA-mCherry
   synuclein alpha (Snca Mouse)
   

   Use

   Lentiviral

   Tags

   NE

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA4/TO/Strep-HA-ZAK beta

   Plasmid

   #141195

   Purpose

   Expresses Strep-HA-ZAK beta in mammalian cells, can be used to make inducible cell line

   Depositor

   Simon Bekker-Jensen

   Article

   Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

   Insert

   MAP3K20 isoform 2 (MAP3K20 Human)
   

   Tags

   Strep, HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB-tetO(hCMV1)-HA-Tet1(201R2)-IV

   Plasmid

   #102421

   Purpose

   Inducible expression of siRNA resistant mouse Tet1-201 (Ensembl transcript ENSMUST00000050826.13) with HA-tag and IRES-Venus in piggyBac (PB) transposon vector

   Depositor

   Kian Peng Koh

   Article

   Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

   Insert

   Tet1 (Tet1 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Modified at the Dharmacon SMARTpool siRNA #2 targ…

   Promoter

   TetO-CMV

   Available Since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB-tetO(hCMV1)-HA-Tet1mHxD(201R2)-IV

    Plasmid

    #102422

    Purpose

    Inducible expression of siRNA resistant mouse Tet1-201 (ENSMUST00000050826.13) with mutated catalytic domain (H1620Y & D1622A), HA-tag and IRES-Venus in piggyBac (PB) transposon vector

    Depositor

    Kian Peng Koh

    Article

    Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

    Insert

    Tet1 catalytic domain mutant (Tet1 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    H1620Y and D1622A mutations in Tet1 catalytic dom…

    Promoter

    TetO-CMV

    Available Since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSIN-hSNCA-NE

    Plasmid

    #102366

    Purpose

    Lentiviral overexpression of synuclein alpha

    Depositor

    Shu Leong Ho

    Article

    Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

    Insert

    synuclein alpha (SNCA Human)
    

    Use

    Lentiviral

    Tags

    NE

    Expression

    Mammalian

    Mutation

    A silent mutation (333bp downstream of ATG) was i…

    Promoter

    EF-1a

    Available Since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA4/TO/Strep-HA-ZAKalpha_deltaCTD

    Plasmid

    #141196

    Purpose

    Expresses Strep-HA-ZAKalpha_deltaCTD in mammalian cells, can be used to make inducible cell lines

    Depositor

    Simon Bekker-Jensen

    Article

    Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

    Insert

    MAP3K20 (MAP3K20 Human)
    

    Tags

    Strep, HA

    Expression

    Mammalian

    Mutation

    deletion of last 27 amino acids

    Promoter

    CMV

    Available Since

    June 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcdna6.2- myc ULK1 k46I

    Plasmid

    #27630

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2011 Jan 28;331(6016):456-61. d

    Insert

    mouse myc ULK1 K46I (Myc Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Initial M from ULK1 removed. Silent mutation at A…

    Available Since

    Feb. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA4/TO/Strep-HA-ZAKalpha_K45A

    Plasmid

    #141194

    Purpose

    Expresses kinase-dead Strep-HA-ZAK alpha in mammalian cells, can be used to make inducible cell line

    Depositor

    Simon Bekker-Jensen

    Article

    Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

    Insert

    MAP3K20 (MAP3K20 Human)
    

    Tags

    Strep, HA

    Expression

    Mammalian

    Mutation

    Changed Lysine 45 to Alanine

    Promoter

    CMV

    Available Since

    June 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA4/TO/Strep-HA-ZAKalpha_deltaSdeltaCTD

    Plasmid

    #141198

    Purpose

    Expresses Strep-HA-ZAKalpha_deltaSdeltaCTD in mammalian cells, can be used to make inducible cell lines

    Depositor

    Simon Bekker-Jensen

    Article

    Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

    Insert

    MAP3K20 (MAP3K20 Human)
    

    Tags

    Strep, HA

    Expression

    Mammalian

    Mutation

    deletion of last 27 amino acids and amino acids 6…

    Promoter

    CMV

    Available Since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. A3Ai E72A-Cas9n-UGI-NLS

    Plasmid

    #109430

    Purpose

    Expresses catalytically inactive human APOBEC3A with an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A E72A Catalytic Mutant (APOBEC3A Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Insertion of an L1 intron into the coding sequenc…

    Promoter

    CMV

    Available Since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQCXIN- myc ULK1 K46I

    Plasmid

    #27627

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2011 Jan 28;331(6016):456-61. d

    Insert

    mouse myc ULK1 K46I (Myc Mouse)
    

    Use

    Retroviral

    Tags

    myc

    Mutation

    Initial M from ULK1 removed. Silent mutation at A…

    Available Since

    April 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221 - myc ULK1 k46I

    Plasmid

    #27624

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2011 Jan 28;331(6016):456-61. d

    Insert

    mouse myc ULK1 K46I (Myc Mouse)
    

    Use

    Gateway donr vector

    Tags

    myc

    Mutation

    Initial M from ULK1 removed. Silent mutation at A…

    Available Since

    Feb. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA4/TO/Strep-HA-ZAKalpha_deltaS

    Plasmid

    #141197

    Purpose

    Expresses Strep-HA-ZAKalpha_deltaS in mammalian cells, can be used to make inducible cell lines

    Depositor

    Simon Bekker-Jensen

    Article

    Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

    Insert

    MAP3K20 (MAP3K20 Human)
    

    Tags

    Strep, HA

    Expression

    Mammalian

    Mutation

    deletion of amino acids 670-712

    Promoter

    CMV

    Available Since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB CRIV Gc Spike H6

    Plasmid

    #240163

    Purpose

    Plasmid for making PiggyBac stable cell line expressing Cristoli virus (CRIV) glycoprotein Gc spike with a C-terminal His6 tag

    Depositor

    Stephen Graham

    Article

    Merchant et al EMBO Mol Med. 2025 Aug 11. doi: 10.1038/

    Insert

    CRIV Gc spike
    

    Use

    Piggybac

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    contains residues 478-906 only

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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