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pMSCV40-EF1a-hBCL10(1-185)-SO

Plasmid

#75258

Purpose

retroviral expression vector for expression of StrepOne-Tagged human BCL10 (AS 1-185)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

Bcl10 (BCL10 Human)

Use

Retroviral

Tags

StrepOne Tag

Expression

Mammalian

Mutation

AS 1-185 (w/o C-terminus)

Promoter

pMSCV-LTRs, EF1a

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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attB-GFP-Tet1d834-1053-Poly(A)

Plasmid

#65561

Purpose

Plasmid for Bxb1-mediated recombination of the GFP-Tet1d834-1053 cDNA into a MIN-tagged locus

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Tet1 (Tet1 Mouse)

Use

Mouse Targeting; Bxb1

Tags

GFP

Expression

Mammalian

Mutation

del(834-1053)

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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NFATc1-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75238

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc1 (1/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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NFATc1-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75239

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc1 (2/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pLJC2-HK2 (D209A, D657A)-3xFLAG

Plasmid

#239244

Purpose

HK2 lentiviral overexpression vector

Depositor

Jason Cantor

Article

Huggler et al Nat Metab. 2026 Jan 16. doi: 10.1038/s42

Insert

HK2 (HK2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Insert contains the set of silent mutations descr…

Promoter

CMV

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pCBS-5753

Plasmid

#249325

Purpose

To express the EPEC dDarT2(DLAA)-nScCas9(D10A) fusion protein in experiments for Append Editing

Depositor

Chase Beisel

Article

Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038

Insert

EPEC dDarT2(DLAA)-nScCas9(D10A)

Expression

Yeast

Mutation

DarT2(G49D, M86L, R92A, E,170A, R193A) and nScCas…

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pTB106-Hyg

Plasmid

#236779

Purpose

Expression of Cas9 cytosine base editor (CBE), T7 RNAP, Cas12a and hygromycin selection marker. The CBE contains a ssDNA-DBD from the L. major RAD51 protein. This plasmid is transfected as episome.

Depositor

Tom Beneke

Article

Herrmann May et al eLife. 2025 Feb 24;13:RP97437. doi: 10.7

Insert

hyBE4max (CBE); Cas12a (Acidaminococcus sp. BV3L6)-P2A-T7 RNAP

Use

CRISPR

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pTB107-Phleo

Plasmid

#236782

Purpose

Expression of Cas9 cytosine base editor (CBE), T7 RNAP, Cas12a and phleomycin selection marker. The CBE contains a ssDNA-DBD from the H. sapiens RAD51 protein. This plasmid is transfected as episome.

Depositor

Tom Beneke

Article

Herrmann May et al eLife. 2025 Feb 24;13:RP97437. doi: 10.7

Insert

hyBE4max (CBE); Cas12a (Acidaminococcus sp. BV3L6)-P2A-T7 RNAP

Use

CRISPR

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV38-2/DV8

Plasmid

#237992

Purpose

Encodes TRAV38-2/DV8 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBC-P2A-TRAV38-2/DV8 (TRAV38-2DV8 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV36/DV7

Plasmid

#237990

Purpose

Encodes TRAV36/DV7 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBC-P2A-TRAV36/DV7 (TRAV36DV7 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV23/DV6

Plasmid

#237980

Purpose

Encodes TRAV23/DV6 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBC-P2A-TRAV23/DV6 (TRAV23DV6 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV18

Plasmid

#237975

Purpose

Encodes TRAV18 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBC-P2A-TRAV18 (TRAV18, TRBC1 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-MYC-GFP-IRES-mCherry

Plasmid

#231232

Purpose

MYC stability reporter construct (expressing c-myc 2) for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

Depositor

Michael Rape

Article

Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

Insert

MYC (MYC Human)

Expression

Mammalian

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-MCRS1-GFP-IRES-mCherry

Plasmid

#231231

Purpose

MCRS1 stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

Depositor

Michael Rape

Article

Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

Insert

MCRS1 (MCRS1 Human)

Expression

Mammalian

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pKM-U6-reRNA-mmDNMT1

Plasmid

#205635

Purpose

reRNA guide targeting Mus Musculus DNMT1

Depositor

Gerald Schwank

Article

Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

Insert

reRNA-mmDNMT1 (Dnmt1 Mouse)

Expression

Mammalian

Promoter

U6

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-806_HA-GD2-28z_CAR_RFP-TFAP4

Plasmid

#207493

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

RFP-TFAP4, HA-GD2-28z_CAR (TFAP4 Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-851_HA-GD2-28z_HA-TFAP4

Plasmid

#207501

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

HA-TFAP4, HA-GD2-28z_CAR (TFAP4 Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-V1-CD20-Puro

Plasmid

#209755

Purpose

Lentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1. Specifically, the variant 1 mRNA isoform.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-V2-CD20-Puro

Plasmid

#209756

Purpose

Lentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1. Specifically, the variant 2 mRNA isoform.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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1529_pAAV-CB-FKBP12-BirA-HA

Plasmid

#208200

Purpose

Express the murine FKBP12-BirA-HA fusion protein in AAV vector

Depositor

Susan Hamilton

Article

Lee et al Commun Biol. 2023 Sep 14;6(1):942. doi:

Insert

Fkbp12 (Fkbp1a Mouse)

Use

AAV

Promoter

Chicken beta-actin

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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