We narrowed to 18,992 results for: Met;

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pLBC0040

Plasmid

#172779

Purpose

Level 0 module containing NT2_cYFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS C-terminal eYFP (175-238)

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0041

Plasmid

#172780

Purpose

Level 0 module containing CT_cYFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS C-terminal eYFP (175-238)

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pPD355 VPR-FRB (NLS)

Plasmid

#138847

Purpose

Constitutive expression of VPR-FRB under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VPR-FRB

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCoofy42

Plasmid

#55190

Purpose

Pichia pastoris expression vector for parallel SLIC cloning. C-terminal tags can be added during SLIC.

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His10-Stop-OneStrep-Stop-S-tag-Stop-CBP-HPC4-Stop…

Expression

Yeast

Promoter

AOX1

Available Since

July 28, 2014

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPglu-2uDelta13

Plasmid

#13561

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16F D152A Delta13

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

F16A, D152A in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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MXS_PGK

Plasmid

#62414

Purpose

MXS_chaining vector with PGK

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

PGK Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pVH234-Tier1-PmPGK1-tTA

Plasmid

#169574

Purpose

Tier-1 vector encoding PmPGK1-driven tTA expression (PmPGK1-tTA-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled transactivator

Expression

Mammalian

Promoter

PPGK

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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SNAP-EF(N)-60nmSAH-GFP(1-10)

Plasmid

#61663

Purpose

2x-30nm SAH scaffold with EF Hand and split GFP attachment sites

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

SNAP-EF(N)-60nmSAH-GFP(1-10)

Expression

Mammalian

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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pET28 Set9 mutant

Plasmid

#24083

Depositor

Danny Reinberg

Article

Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

Insert

Set9 (SETD7 Human)

Tags

FLAG and His

Expression

Bacterial

Mutation

H297A

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
mEos2-Coronin1B-C-10

Plasmid

#57362

Purpose

Localization: Cytoskeleton, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Coronin1B (CORO1B Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCGN R-Ras 41A

Plasmid

#14730

Depositor

Adrienne Cox

Article

Fiordalisi et al Methods Enzymol. 2001;332:3-36. doi: 10.

Insert

R-ras (RRAS Human)

Tags

HA

Expression

Mammalian

Mutation

41A

Available Since

Aug. 29, 2007

Availability

Academic Institutions and Nonprofits only
SNAP-EF(N)-5nmSAH-GFP(1-10)

Plasmid

#61659

Purpose

5nm SAH scaffold with EF Hand and split GFP attachment sites

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

SNAP-EF(N)-5nmSAH-GFP(1-10)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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mEGFP-30nmSAH-GFP(1-10)

Plasmid

#61677

Purpose

GFP-labeled SAH scaffold with split GFP attachment site

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

mEGFP-30nmSAH-GFP(1-10)

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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YIp211-P30-His-POL30-3'

Plasmid

#99546

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA); URA3 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Tags

6xHis

Expression

Yeast

Promoter

POL30

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pTK64_GFP-NuMA-Bonsai

Plasmid

#46351

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

NuMA deleted 414-1549 (NUMA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acid 414-1549; L1589F mutation in N…

Promoter

pBABE

Available Since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pBD21_033

Plasmid

#109150

Purpose

pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of Sirius

Depositor

Ron Weiss

Article

Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1

Inserts

mRFP
Sirius

Use

Synthetic Biology

Expression

Bacterial and Mammalian

Promoter

CMV and pTet

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pBD21_036

Plasmid

#109153

Purpose

pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of mKO2

Depositor

Ron Weiss

Article

Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1

Inserts

mRFP
mKO2

Use

Synthetic Biology

Expression

Bacterial and Mammalian

Promoter

CMV and pTet

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pBD21_034

Plasmid

#109151

Purpose

pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of TagBFP

Depositor

Ron Weiss

Article

Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1

Inserts

mRFP
TagBFP

Use

Synthetic Biology

Expression

Bacterial and Mammalian

Promoter

CMV and pTet

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pFS177

Plasmid

#12380

Depositor

Nick Rhind

Article

Sivakumar et al Methods. 2004 Jul . 33(3):213-9.

Insert

human equilibrating nucleoside transporter 1 (SLC29A1 Human)

Expression

Yeast

Mutation

N81S and M84V

Available Since

Aug. 24, 2006

Availability

Academic Institutions and Nonprofits only

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EZ-L767

Plasmid

#131773

Purpose

PADH1_sFUS_Fusion Red_PixD_TACT1

Depositor

Jared Toettcher

Article

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

PADH1_sFUS_Fusion Red_PixD_TACT1

Use

Integration into δ-sites

Expression

Yeast

Promoter

PADH1

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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Pyb1301(JBEI-11629)

Plasmid

#87959

Purpose

jStack yeast assembly into plant vectors, Level 2 pYB Vector, Hygro selection

Depositor

Dominique Loque

Article

Shih et al Nat Commun. 2016 Oct 26;7:13215. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pRetroSuper shSmad4J puro

Plasmid

#19152

Depositor

Joan Massague

Article

Padua et al Cell. 2008 Apr 4. 133(1):66-77.

Insert

small hairpin RNA against SMAD family member 4 (SMAD4 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Oct. 29, 2008

Availability

Academic Institutions and Nonprofits only
pCfB8383

Plasmid

#126917

Purpose

Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of tHMG1

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

tHMG1

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-R-Hras

Plasmid

#180579

Purpose

ddRFP sensor of Hras

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddRFP sensor of Hras

Use

AAV

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-G-Rac1

Plasmid

#180581

Purpose

ddGFP sensor of Rac1

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Rac1

Use

AAV

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pJet1.2 Tev-Halo tag

Plasmid

#117124

Purpose

C-terminal Tev Halo-Tag

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

Tev-Halo-tag Tag

Use

Golden gate donor vector for gene targeting in ba…

Tags

Tev-Halo-Tag

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pMOD_D5802

Plasmid

#176880

Purpose

High constitutive expression of Firefly Luciferase in plants

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

AtUbi10:Luc+:RbcsE9

Use

Luciferase

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pEPYCeGM0001

Plasmid

#196141

Purpose

Gateway entry vector with Arabidopsis TGA1 CDS and c-terminal HiBiT tag

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

AtTGA1_HiBit

Use

Gateway entry vector

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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P0050::rpaA (KmR)

Plasmid

#102321

Purpose

drives expression of rpaA from an rpaA-independent promoter (kanamycin resistance casette)

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

RpaA with p0050 promoter (+ kanamycin resistance casette)

Use

Cloning vector

Promoter

-

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pET29b_Qcat9

Plasmid

#45137

Depositor

Christopher Petzold

Article

Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

Insert

E-coli-pathways-QconCAT9

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pET29b_Qcat10

Plasmid

#45138

Depositor

Christopher Petzold

Article

Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

Insert

E-coli-pathways-QconCAT10

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pET29b_Qcat3

Plasmid

#45131

Depositor

Christopher Petzold

Article

Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

Insert

E-coli-pathways-QconCAT3

Tags

5XHis

Expression

Bacterial

Promoter

T7

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL1P3_AtU626Ter26

Plasmid

#191771

Purpose

Cas9 guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

SpCas9 guide scaffold with AtU626 promoter/terminator

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLBT0190

Plasmid

#172815

Purpose

Level 1 module containing OEP7-mTRQ_P4 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-OEP7-mTRQ-3U+TER35S

Use

Synthetic Biology

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0100

Plasmid

#172789

Purpose

Level 0 module containing CT_Stop

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

Spacer module with stop codons.

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0150

Plasmid

#172795

Purpose

Level 0 module containing NT2_TurboID

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

TurboID with 3XHA tag

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0050

Plasmid

#172781

Purpose

Level 0 module containing NT2_cYFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS 3XFLAG tag N-terminal eYFP (1-174)

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBC0051

Plasmid

#172782

Purpose

Level 0 module containing CT_3XFLAGnYFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS 3XFLAG tag N-terminal eYFP (1-174)

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
His6_BcLOV4_Q355N_mCherry_BamUK

Plasmid

#119762

Purpose

Encodes mCherry-tagged BcLOV4 permanently-"lit" mutant with Q355N mutation for expression in bacterial cell

Depositor

Brian Chow

Article

Glantz et al Methods Enzymol. 2019;622:249-270. doi:

Insert

BcLOV4-Q355N_mCherry

Tags

mCherry

Expression

Bacterial

Mutation

Mutated glutamine at position 355 to asparagine

Promoter

T7

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pBzCas13b

Plasmid

#164862

Purpose

Inducible expression of BzCas13b nuclease in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

Bergeyella zoohelcum Cas13b

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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Showing: 7521 - 7560 of 18992 results

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