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pJW1841

Plasmid

#154338

Purpose

Promoter reporter

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

SV40 NLS::mScarlet_I(dpi)::-tbb-2 3'UTR

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pET151-GST-PP-RPN13 (19-132) K97E

Plasmid

#125825

Purpose

Expresses GST-RPN13 (19-132) K97E in E.coli

Depositor

Chris Hill

Article

Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

Insert

RPN13 (ADRM1 Human)

Tags

GST

Expression

Bacterial

Mutation

changed lysine 97 to glutamate

Promoter

T7

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pET151-GST-PP-RPN13 (19-132) K83A

Plasmid

#125829

Purpose

Expresses GST-RPN13 (19-132) K83A in E.coli

Depositor

Chris Hill

Article

Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

Insert

RPN13 (ADRM1 Human)

Tags

GST

Expression

Bacterial

Mutation

changed lysine 83 to alanine

Promoter

T7

Available Since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pET151-GST-PP-RPN13 (19-132) K97A

Plasmid

#125823

Purpose

Expresses GST-RPN13 (19-132) K97A in E.coli

Depositor

Chris Hill

Article

Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

Insert

RPN13 (ADRM1 Human)

Tags

GST

Expression

Bacterial

Mutation

changed lysine 97 to alanine

Available Since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pET151-GST-PP-RPN13 (19-132) K97D

Plasmid

#125824

Purpose

Expresses GST-RPN13 (19-132) K97D in E.coli

Depositor

Chris Hill

Article

Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

Insert

RPN13 (ADRM1 Human)

Tags

GST

Expression

Bacterial

Mutation

changed lysine 97 to aspartate

Promoter

T7

Available Since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pJL1

Plasmid

#117429

Purpose

Expression of ARL1

Depositor

Richard Kahn

Article

Van Valkenburgh et al J Biol Chem. 2001 Jun 22;276(25):22826-3

Insert

ARL1 (ARL1 Human)

Expression

Bacterial

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-hygro-eGFP-STOP-HEC1

Plasmid

#114026

Purpose

Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines. Due to insertion of a STOP-codon after the eGFP-tag, this plasmid only expressed eGFP.

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-STOP-HEC1 (NDC80 Human)

Expression

Mammalian

Mutation

Start codon of Hec1 is mutated to stop codon

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac-LacI-LAP-HEC1

Plasmid

#114033

Purpose

Production of baculovirus with LacI-LAP-HEC1

Depositor

Geert Kops

Article

Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

Insert

LacI-LAP-HEC1 (NDC80 Human)

Expression

Insect

Mutation

none

Promoter

CMV

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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PX458_KDM3A_1

Plasmid

#86321

Purpose

Encodes gRNA for 3' target of human KDM3A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KDM3A (KDM3A Human)

Use

CRISPR

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only
PX458_KDM3A_2

Plasmid

#86322

Purpose

Encodes gRNA for 3' target of human KDM3A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KDM3A (KDM3A Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST14-ARL1

Plasmid

#67374

Purpose

Expression of native hArl1 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL1 (ARL1 Human)

Expression

Bacterial

Mutation

bp 231 C to T; silent mutation

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pFastBac-Gm129-Flag

Plasmid

#53766

Purpose

baculovirus expression of Gm129

Depositor

Aziz Sancar

Article

Annayev et al J Biol Chem. 2014 Feb 21;289(8):5013-24.

Insert

Gm129 (Ciart Mouse)

Tags

FLAG

Expression

Insect

Promoter

Polyhedrin

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only
pGL3-PIM2mut3

Plasmid

#53742

Purpose

promoter region of PIM2 lacking the 2 forkhead binding sites

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

PIM2 (PIM2 Human)

Use

Luciferase; Reporter vector

Mutation

deletion of 2 forkhead binding sites (bp# 395-497…

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
psiCheck-DNAJA43'UTR

Plasmid

#41847

Depositor

Sohail Tavazoie

Article

Pencheva et al Cell. 2012 Nov 21;151(5):1068-82. doi: 1

Insert

DNAJA4 3'UTR (DNAJA4 Human)

Use

Luciferase

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
BacPAK Myc-Adrm1

Plasmid

#19420

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

Insert

adhesion regulating molecule 1 (ADRM1 Human)

Tags

His and Myc

Expression

Insect

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
BM28

Bacterial Strain

#201047

Purpose

MG1655-derived Escherichia coli isolate obtained from adaptive laboratory evolution to increasing incubation temperatures. Also carries a pBR325 plasmid carrying groESL called pOF39.

Depositor

Jeannette Winter

Article

McGuire et al BMC Genomics. 2023 Mar 27;24(1):154. doi

Bacterial Resistance

Ampicillin and Kanamycin

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only
Guzit

Bacterial Strain

#196902

Purpose

Guzit is a RNase HI(rnhA) His-tagged E. coli strain made for a cell-free expression system.

Depositor

Kate Adamala

Article

Sato et al Biotechnol Bioeng. 2023 Jul;120(7):1986-

Bacterial Resistance

Kanamycin

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only
pPB_TRE3G_scFV-sfGFP-Dot1LCD

Plasmid

#235582

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Dot1l fused with scFV to programme H3K79me2

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Dot1l (Dot1l Mouse)

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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human beta-catenin pcDNA3

Plasmid

#16828

Purpose

Mammalian expression of human beta-catenin with Flag tag

Depositor

Eric Fearon

Article

Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

Insert

beta-catenin (CTNNB1 Human)

Tags

Flag

Expression

Mammalian

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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integrin alpha9 pcDNA1 neo

Plasmid

#13585

Depositor

Dean Sheppard

Article

Yokosaki et al J Biol Chem. 1994 Oct 28. 269(43):26691-

Insert

integrin alpha 9 (ITGA9 Human)

Expression

Mammalian

Available Since

Jan. 8, 2007

Availability

Academic Institutions and Nonprofits only

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