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CRF1R-GFP

Plasmid

#208912

Purpose

encodes the human corticotropin releasing factor 1 receptors, tagged with YFP

Depositor

Christian Gruber

Article

Fahradpour et al Front Pharmacol. 2017 Sep 25;8:616. doi:

Insert

CRHR1 (CRHR1 Human)

Tags

EYFP

Expression

Mammalian

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-10nm-EBFP2

Plasmid

#231184

Purpose

Expresses a synthetic linker, tagged with EBFP2, Stabilizing the ER-mitochondrial distance at 10 nm

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

10nm-EBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLPS1-iLID::EGFP::FTH1 (pBS1042)

Plasmid

#185289

Purpose

For the mammalian expression of the synthetic protein iLID::eGFP::FTH1 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

iLID::eGFP::FTH1

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot9shRNA2res_AG

Plasmid

#148828

Purpose

Mammalian Expression of HsNot9-shRNA2res

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

HsNot9-shRNA2res (CNOT9 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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CD-MPR-mCherry-RUSH

Plasmid

#202798

Purpose

RUSH cargo: Str-KDEL_IRES_SBP-mCherry-CD-MPR

Depositor

Juan Bonifacino

Article

Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

Insert

M6PR (M6PR Human)

Tags

mCherry

Expression

Mammalian

Mutation

Changed 40 K (AAA) with R (AGA)

Promoter

CMV

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G_CBP-GFP

Plasmid

#221926

Purpose

Dox-inducible expression of CBP-GFP in mammalian cells by retroviral transduction

Depositor

Daniel Bose

Article

Gelder et al Cell Rep. 2026 Mar 12;45(3):117109. doi:

Insert

CBP (CREBBP Human)

Use

Retroviral

Tags

GFP

Mutation

insertion of CBP-GFP

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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Integrin beta5-2XEGFP

Plasmid

#139779

Purpose

Detecting Integrin beta5 by fluorescence microscopy

Depositor

Staffan Stromblad

Article

Lock et al Nat Cell Biol. 2018 Nov;20(11):1290-1302

Insert

Integrin subunit beta5 (ITGB5 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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qTAG-C-Solo-mStayGold-CANX

Plasmid

#227280

Purpose

Donor template for mStayGold insertion into the C-terminus of the CANX locus. For ER visualization. To be co-transfected with sgRNA plasmid px330-CANX (Addgene #227279)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

CANX Homology Arms flanking a mStayGold Tag (CANX Human)

Use

CRISPR; Donor template

Expression

Mammalian

Promoter

Promoterless

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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Frt-V5-hspB5

Plasmid

#63106

Purpose

Expression of HSPB5 (small HSP) tagged with V5 in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb5 (CRYAB Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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GFP KNL1 wt hardened and RVSF->AAAA

Plasmid

#45225

Depositor

Iain Cheeseman, Michael Lampson

Article

Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

Insert

GFP KNL1 with RVSF-AAAA mutation, resistant to KNL1 siRNA (KNL1 Human)

Mutation

RVSF mutated to AAAA

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-CATULIN

Plasmid

#118979

Purpose

Expresses full length of Catulin-GFP (Mus musculus). Contains CMV and T3 promoter for mammalian expression and mRNA production.

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Alpha Catulin (Ctnnal1 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_CRX_P2A_Hygro_Barcode

Plasmid

#120433

Purpose

Barcoded lentiviral vector to express CRX in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

CRX (CRX Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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His6tev CBP HAT (1324-1700)

Plasmid

#99340

Purpose

bacterial expression of His tagged mouse CBP HAT domain (1324-1700)

Depositor

Peter Wright

Article

Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

Insert

mouse CBP HAT domain (1324-1700) (Crebbp Mouse)

Tags

His6tev

Expression

Bacterial

Promoter

T7

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Nlgn3 KI

Plasmid

#131501

Purpose

Endogenous tagging of Neuroligin-3: N-terminal (amino acid position: T37)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Nlgn3, Nlgn3 Rat, Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pSEVA228-pro4IUPi

Plasmid

#122018

Purpose

Expresses IUP pathway genes (ChK, IPK, idi) under constitutive promoter. Kan. resist.

Depositor

Gregory Stephanopoulos

Article

Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

Inserts

Choline kinase (CKI1 Budding Yeast)
Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
isopentenyl-diphosphate Delta-isomerase

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pro4

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNAI-GAL4-CREB

Plasmid

#46769

Purpose

Expression of GAL4 fusion to wild type CREB

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

GAL4(1-147)-CREB (Creb1, GAL4 Budding Yeast, Rat)

Tags

GAL4

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA4c/His-Xpress-HA-hBIG1(E793K)

Plasmid

#79433

Purpose

Expresses N-terminally His6, Xpress and HA-tagged BIG1(E793K) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Shin et al Mol Biol Cell. 2004 Dec;15(12):5283-94.

Insert

BIG1 (ARFGEF1 Human)

Tags

HA, His6, and Xpress

Expression

Mammalian

Mutation

changed Glutamic acid 793 to Lysine

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only
CHRM4-Tango

Plasmid

#66251

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

CHRM4 (CHRM4 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pmBanana-Hyg-CTCF-NtermNES-N1

Plasmid

#131850

Purpose

Expresses the [N-terminus of human CTCF (a.a. 2-265)]-mBanana with a nuclear export signal in mammalian cells

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

CTCF (CTCF Human)

Tags

mBanana

Expression

Mammalian

Mutation

deleted amino acids 796-2181 (the N-terminus is l…

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-hSOX17FNV

Plasmid

#206384

Purpose

Expresses human SOX17FNV in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

Insert

SOX17FNV (SOX17 Human)

Use

Lentiviral

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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