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pcDNA3-YFP

Plasmid

#13033

Purpose

Mammalian expression vector for cloning your gene fused to YFP

Depositor

Doug Golenbock

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Yellow fluorescent protein

Expression

Mammalian

Available since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

33 Articles

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pcDNA3-YFP

Plasmid

#13033

Purpose

Mammalian expression vector for cloning your gene fused to YFP

Depositor

Doug Golenbock

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Yellow fluorescent protein

Expression

Mammalian

Available since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

33 Articles

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pgRNA-humanized

Plasmid

#44248

Purpose

Expression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown

Depositor

Stanley Qi

Publication

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

guide RNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available since

April 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

58 Articles

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pgRNA-humanized

Plasmid

#44248

Purpose

Expression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown

Depositor

Stanley Qi

Publication

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

guide RNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available since

April 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

58 Articles

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pMXs-hSOX2

Plasmid

#17218

Purpose

Retroviral expression of human SOX2

Depositor

Shinya Yamanaka

Publication

Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

Insert

SOX2 (SOX2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

113 Articles

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pMXs-hSOX2

Plasmid

#17218

Purpose

Retroviral expression of human SOX2

Depositor

Shinya Yamanaka

Publication

Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

Insert

SOX2 (SOX2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

113 Articles

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L4440

Plasmid

#1654

Depositor

Andrew Fire

Type

Empty backbone

Use

RNAi

Tags

T7p, T7p, lacZN, OriF1>>, OriF1<<

Expression

Worm

Available since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

94 Articles

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L4440

Plasmid

#1654

Depositor

Andrew Fire

Type

Empty backbone

Use

RNAi

Tags

T7p, T7p, lacZN, OriF1>>, OriF1<<

Expression

Worm

Available since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

94 Articles

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pcDNA3.1(+)Flag-His-ATM wt

Plasmid

#31985

Purpose

Mammalian expression of human ATM with Flag and His tags

Depositor

Michael Kastan

Publication

Canman et al Science. 1998 Sep 11;281(5383):1677-9.

Insert

ATM wild-type (ATM Human)

Tags

Flag and HIS

Expression

Mammalian

Mutation

3' UTR of 400 nucl.

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

39 Articles

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pcDNA3.1(+)Flag-His-ATM wt

Plasmid

#31985

Purpose

Mammalian expression of human ATM with Flag and His tags

Depositor

Michael Kastan

Publication

Canman et al Science. 1998 Sep 11;281(5383):1677-9.

Insert

ATM wild-type (ATM Human)

Tags

Flag and HIS

Expression

Mammalian

Mutation

3' UTR of 400 nucl.

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

39 Articles

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pLKO.1-blast

Plasmid

#26655

Purpose

3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses blasticidin for selection.

Depositor

Keith Mostov

Publication

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

90 Articles

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pLKO.1-blast

Plasmid

#26655

Purpose

3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses blasticidin for selection.

Depositor

Keith Mostov

Publication

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

90 Articles

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pXR003: CasRx gRNA cloning backbone

Plasmid

#109053

Purpose

hU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.

Depositor

Patrick Hsu

Publication

Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

39 Articles

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pXR003: CasRx gRNA cloning backbone

Plasmid

#109053

Purpose

hU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.

Depositor

Patrick Hsu

Publication

Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

39 Articles

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pmRFP-LC3

Plasmid

#21075

Purpose

Mammalian expression of rat LC3 fused to mRFP

Depositor

Tamotsu Yoshimori

Publication

Kimura et al Autophagy. 2007;3(5):452-60.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

mRFP

Expression

Mammalian

Mutation

First ATG is not included.

Available since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

95 Articles

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pmRFP-LC3

Plasmid

#21075

Purpose

Mammalian expression of rat LC3 fused to mRFP

Depositor

Tamotsu Yoshimori

Publication

Kimura et al Autophagy. 2007;3(5):452-60.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

mRFP

Expression

Mammalian

Mutation

First ATG is not included.

Available since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

95 Articles

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pCMV delta R8.2

Plasmid

#12263

Purpose

First-generation packaging plasmid. This plasmid contains HIV accessory genes. Please reach out to your biosafety officer for guidance.

Depositor

Didier Trono

Insert

HIV-1 GAG/POL, Tat and Rev

Use

Lentiviral; Packaging

Expression

Mammalian

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

239 Articles

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pCMV delta R8.2

Plasmid

#12263

Purpose

First-generation packaging plasmid. This plasmid contains HIV accessory genes. Please reach out to your biosafety officer for guidance.

Depositor

Didier Trono

Insert

HIV-1 GAG/POL, Tat and Rev

Use

Lentiviral; Packaging

Expression

Mammalian

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

239 Articles

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pCMV-PEmax

Plasmid

#174820

Purpose

Mammalian expression of SpCas9 PEmax prime editor

Depositor

David Liu

Publication

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PEmax

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

27 Articles

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pCMV-PEmax

Plasmid

#174820

Purpose

Mammalian expression of SpCas9 PEmax prime editor

Depositor

David Liu

Publication

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PEmax

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

27 Articles

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