We narrowed to 399 results for: Tight TRE

1. pInsert-PhoCl-RER

   Plasmid

   #219676

   Purpose

   Caging at cytosolic C-ter of POI: mScarletCD4-PhoCl-RER in pTREtight2

   Depositor

   Helge Ewers

   Article

   Kashyap et al Nat Methods. 2024 Mar 8. doi: 10.1038/s4

   Insert

   CD4mScarlet-PhoCl-RER
   

   Use

   Tags

   mScarlet and PhoCl

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available Since

   June 14, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. aCE89

   Plasmid

   #204494

   Purpose

   TREtight promoted WBeta and PhlF promoted PhiC31 without PEST tags

   Depositor

   Ron Weiss

   Article

   Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

   Inserts

   φC31 integrase
   WBeta recombinase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   PhIF and TREtight

   Available Since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. aCE90

   Plasmid

   #204495

   Purpose

   TREtight promoted WBeta and PhlF promoted PhiC31 with PEST tags

   Depositor

   Ron Weiss

   Article

   Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

   Inserts

   phiC31 recombinase
   WBeta recombinase
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   PhIF and TREtight

   Available Since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TtGNN-TAF4-HFD

   Plasmid

   #106381

   Purpose

   dox inducible expression of GFP fused TAF4 HFD (827-909) with neo resistance

   Depositor

   Christopher Vakoc

   Article

   Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

   Insert

   GFP fused TAF4 HFD (827-909) (Taf4 Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   TREtight

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-UbC-rtTA-Ngn2:2A:EGFP

   Plasmid

   #127288

   Purpose

   all-in-one inducible LV expression construct for Ngn2:2A:EGFP (aka. UNG; for direct neuronal conversion)

   Depositor

   Fred Gage

   Article

   Schafer et al Nat Neurosci. 2019 Feb;22(2):243-255. do

   Insert

   Ngn2:2A:Ascl1 (NEUROG2 Human)
   

   Use

   Lentiviral

   Tags

   Ngn2 and EGFP linked via 2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP

   Plasmid

   #132393

   Purpose

   Exchange cassette containing inducible TauP301L 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site

   Depositor

   Gerold Schmitt-Ulms

   Article

   Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

   Insert

   human TauP301L 2N4R-EGFP (MAPT Synthetic, Human)
   

   Use

   CRISPR and Cre/Lox

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Proline 301 to Leucine (P301L)

   Promoter

   TREtight

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAVS1 CAG rtTA3 TauWT 2N4R-EGFP

   Plasmid

   #132389

   Purpose

   Exchange cassette containing inducible TauWT 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site

   Depositor

   Gerold Schmitt-Ulms

   Article

   Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

   Insert

   human TauWT 2N4R-EGFP (MAPT Synthetic, Human)
   

   Use

   CRISPR and Cre/Lox

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   TREtight

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCHW91015

   Plasmid

   #222455

   Purpose

   Level 1, Position 2 containing Mas::CUP2-VP16::NOS.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Insert

   Mas::CUP2-VP16::NOS (CUP2 )
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   no

   Promoter

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCHW101020

   Plasmid

   #222454

   Purpose

   Level 1, Position 2 containing 35S::CUP2-p65::NOS.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Insert

   35S::CUP2-p65::NOS (CUP2 )
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   no

   Promoter

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCHW00011

    Plasmid

    #222439

    Purpose

    Level 0, CDSns module (AATG-TTCG) containing S. cerevisiae CUP2.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Insert

    CUP2 (Saccharomyces cerevisiae) (CUP2 Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    no

    Promoter

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28b-AF0623

    Plasmid

    #30164

    Depositor

    SeWon Suh

    Article

    Kim do et al Acta Crystallogr Sect F Struct Biol Crys

    Insert

    AF0623 (lig Archaeoglobus fulgidus)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pUt-mPF-BACH1 Cas9-Resistant_AmpR

    Plasmid

    #199221

    Purpose

    LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Positive-Feedback circuit (mPF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection. 

    Depositor

    Gabor Balazsi

    Article

    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

    Insert

    rtTA-Advanced::P2A::EGFP::BACH1 (BACH1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    silient mutation at nucleic acid 177 C changed to…

    Promoter

    tight TRE promoter

    Available Since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-UbC-rtTA-Ngn2:2A:Ascl1

    Plasmid

    #127289

    Purpose

    all-in-one inducible LV expression construct for Ngn2:2A:Ascl1 (aka UNA; for direct neuronal conversion)

    Depositor

    Fred Gage

    Article

    Herdy et al Elife. 2019 May 17;8. pii: 41356. doi: 1

    Inserts

    Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
    Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
    

    Use

    Lentiviral

    Tags

    Ngn2 and Ascl1 linked via 2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLENTI_TetOn_Hu_BIRC5(WT)

    Plasmid

    #136348

    Purpose

    Lentiviral inducible expression (TetOn) of human BIRC5

    Depositor

    Guillermo de Cárcer

    Insert

    BIRC5 (BIRC5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLENTI_TetOn_Hu_MAD2(WT)

    Plasmid

    #136347

    Purpose

    Lentiviral inducible expression (TetOn) of human MAD2

    Depositor

    Guillermo de Cárcer

    Insert

    MAD2L1 (MAD2L1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLENTI_TetOn_Hu_ECT2(WT)

    Plasmid

    #136339

    Purpose

    Lentiviral inducible expression (TetOn) of human ECT2

    Depositor

    Guillermo de Cárcer

    Insert

    ECT2 (ECT2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLVX ALIX-GFP WT

    Plasmid

    #138582

    Purpose

    Expresses fluorescent ALIX in mammalian cells

    Depositor

    Wesley Sundquist

    Insert

    ALIX (PDCD6IP Human)
    

    Use

    Lentiviral

    Tags

    GFP and h30

    Expression

    Mammalian

    Mutation

    Promoter

    TREtight

    Available Since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCW57.1-MAT2A

    Plasmid

    #100521

    Purpose

    Dox-repressible cDNA expression of MAT2A (dox-off/ tet-off all-in-one vector)

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    MAT2A (MAT2A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTight TRE

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLVX ALIX-GFP S718/721A

    Plasmid

    #138583

    Purpose

    Expresses fluorescent ALIX S718/721A in mammalian cells

    Depositor

    Wesley Sundquist

    Insert

    ALIX (PDCD6IP Human)
    

    Use

    Lentiviral

    Tags

    GFP and h30

    Expression

    Mammalian

    Mutation

    changed serines 718 and 721 to alanines

    Promoter

    TREtight

    Available Since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLVX ALIX-GFP S718/721/E

    Plasmid

    #138584

    Purpose

    Expresses fluorescent ALIX S718/721E in mammalian cells

    Depositor

    Wesley Sundquist

    Insert

    ALIX (PDCD6IP Human)
    

    Use

    Lentiviral

    Tags

    GFP and h30

    Expression

    Mammalian

    Mutation

    changed serines 718 and 721 to glutamic acids

    Promoter

    TREtight

    Available Since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge