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Showing: 361 - 380 of 7736 results

  1. R777-E312 Hs.SHC3-nostop

    Plasmid
    #70596
    Purpose
    Gateway ORF clone of codon optimized human SHC3 [NM_016848.5] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SHC3 (SHC3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70596-plasmid-map-sequence-id-126045

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  2. R777-E310 Hs.SHC2-nostop

    Plasmid
    #70594
    Purpose
    Gateway ORF clone of codon optimized human SHC2 [NM_012435.2] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SHC2 (SHC2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70594-plasmid-map-sequence-id-126043

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  3. R777-E311 Hs.SHC3

    Plasmid
    #70595
    Purpose
    Gateway ORF clone of codon optimized human SHC3 [NM_016848.5] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SHC3 (SHC3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70595-plasmid-map-sequence-id-126044

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  11. pCMV-T7-SpRY-P2A-mScarlet (MNW002)

    Plasmid
    #174137
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R); C-terminal bi-partite NLS, 3x flag tag, P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpRY with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpG=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317…
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174137-plasmid-map-sequence-id-341916

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  12. pCS2+/N-SNAPf

    Plasmid
    #184415
    Purpose
    vector backbone to clone insert downstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesis
    Depositor
    Ann Miller
    Article
    Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do
    Type
    Empty backbone
    Use
    For mrna synthesis
    Tags
    SNAPf-tag
    Expression
    Mutation
    Codon optimized for expression in Xenopus laevis …
    Promoter
    Available since
    Aug. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    184415-plasmid-map-sequence-id-365684

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  13. pCS2+/C-SNAPf

    Plasmid
    #184416
    Purpose
    vector backbone to clone insert upstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesis
    Depositor
    Ann Miller
    Article
    Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do
    Type
    Empty backbone
    Use
    For mrna synthesis
    Tags
    SNAPf-tag
    Expression
    Mutation
    Codon optimized for expression in Xenopus laevis …
    Promoter
    Available since
    Aug. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    184416-plasmid-map-sequence-id-362158

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  14. pCMV-T7-SpCas9-NG-P2A-mScarlet (MNW005)

    Plasmid
    #174140
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174140-plasmid-map-sequence-id-341918

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  19. PTet-F30-Pepper aptamer-UTR1-mTurq2

    Plasmid
    #227645
    Purpose
    pTet-driven expression of F30-scaffolded Pepper aptamer and mTurquoise2 for quantifying cell-free RNA and protein synthesis. The mTurq2 has a GGGGS linker followed by a His6x tag.
    Depositor
    Richard Murray
    Article
    Kapasiawala et al bioRxiv 2023.03.22.533877
    Insert
    F30-scaffolded Pepper aptamer and mTurquoise2 (codon optimized for E. coli B using IDT Codon Optimizer)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    227645-plasmid-map-sequence-id-452394

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  20. HDM-SARS2-Spike-delta21

    Plasmid
    #155130
    Purpose
    Encodes SARS-CoV-2 Spike with 21 aa C-terminal deletions for lentiviral pseudotyping
    Depositor
    Jesse Bloom
    Article
    Crawford et al Viruses. 2020 May 6;12(5). pii: v1205051
    Insert
    SARS-CoV-2 Spike-delta21 (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Codon optimized to H. sapiens using IDT codon opt…
    Promoter
    CMV
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    155130-plasmid-map-sequence-id-306280

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Showing: 361 - 380 of 7736 results