We narrowed to 40,059 results for: Eng
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pCRISPomyces-1
Plasmid#61736PurposeStreptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNADepositorInsertssSpCas9
tracrRNA
crRNA cassette
UseCRISPRTagsExpressionBacterialMutationBbsI-flanked lacZ cassette inserted in place of s…Promotergapdhp(EL), rpsLp(CF), and rpsLp(XC)-BbsIAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-PH1(WT)
Plasmid#86599PurposeExpresses eGFP-tagged PH(WT) domain of human Akt1DepositorInsertAkt1 PH domain (AKT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
phMYT1L-N106
Plasmid#60861PurposeExpresses MYT1L under the EF1a promoterDepositorInsertMYT1L (MYT1L Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQE-80L iLID (C530M)
Plasmid#60408PurposeE. coli expression of iLID (C530M)DepositorInsertiLID C530M
UseTagsHisExpressionBacterialMutationC530MPromoterAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHAGE DN Cullin2
Plasmid#41912DepositorInsertCUL2 (CUL2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBK-YE1-BE3
Plasmid#85174PurposeExpresses YE1-BE3 in mammalian cellsDepositorInsertYE1-BE3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTD-C_eYFPTwinStrep
Plasmid#45942Purposeexpresses E.coli codon optimized EYFP-Twin-Strep fusion proteinDepositorInsertsynthetic eYFP (codon usage adapted to E.coli K12)
UseTagsTwin-Strep-tagExpressionBacterialMutationPromoterlacIq/PtrcAvailable sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTagBFP-Akt1
Plasmid#86594PurposeExpresses TagBFP-tagged human Akt1(WT)DepositorInsertAkt1 (AKT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBK-YEE-BE3
Plasmid#85177PurposeExpresses YEE-BE3 in mammalian cellsDepositorInsertYEE-BE3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Myc CDC4 R465C
Plasmid#16653DepositorInsertCDC4 R465C (FBXW7 Human)
UseTagsmycExpressionMammalianMutationR465CPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-human lyn–GFP
Plasmid#35958PurposeExpresses human lyn protein codon optimized for zebrafish expression.DepositorInsertLYN (LYN Human)
UseTagsEGFPExpressionMammalianMutationwtPromotercmvAvailable sinceApril 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBS human IRS-1
Plasmid#11359DepositorInsertIRS-1 (IRS1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pJK-caCas9-NatMX-Neut5L
Plasmid#89576PurposecaCas9 integrating vector into the Neut5L locusDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pR1G108CP20X
Plasmid#85086PurposeExpresses PCNA1 variant (G108C) fused to putidaredoxin reductase and putidaredoxin in E. coliDepositorInsertThe G108C variant of PCNA1 fused to putidaredoxin reductase and putidaredoxin
UseTagsHis6 tagExpressionBacterialMutationG108C in PCNA1PromoterT7 promoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetO-Nanog
Plasmid#40800DepositorInsertNanog (Nanog Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterMinimal CMV + TetOAvailable sinceOct. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHAGE DN Cullin4A
Plasmid#41914DepositorInsertCUL4A (CUL4A Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
STIMATE-mCherry
Plasmid#70676PurposeExpresses STIMATE or TMEM110 in mammalian cellsDepositorInsertSTIMATE (TMEM110 Human)
UseTagsmcherryExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ233 (dLbCpf1-SRDX)
Plasmid#86211PurposeDeactivated LbCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmidDepositorInsertLbCpf1
UseCRISPRTags5' and 3' NLS and SRDX repressorExpressionPlantMutationPromoterAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE DN Cullin4B
Plasmid#41915DepositorInsertCUL4B (CUL4B Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDR-GFPuniv
Plasmid#46085PurposeIn vivo recombination assay fluorescent reporterDepositorInsertDR-GFPuniv reporter
UseRecombination reporter plasmidTagsExpressionMammalianMutationE5G and K163R mutations in EGFP relative to wild-…PromoterAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
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FUW-TetO-Gata4
Plasmid#41084DepositorInsertGata4 (Gata4 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTetO + minimal CMVAvailable sinceNov. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGL4 mIL-17 0.6kb promoter
Plasmid#20126DepositorInsertmIL-17 (Il17a Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJan. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET16b-Trx CC32/35SS
Plasmid#36946DepositorInsertThioredoxin 1 (TXN Human)
UseTagsHis tagExpressionBacterialMutationboth active site cysteines (32,35) mutated to ser…PromoterT7Available sinceMay 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAV103
Plasmid#63193PurposepRS403 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionWorm and YeastMutationPromoterAvailable sinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJZC43
Plasmid#66565PurposesgRNA + 2XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherryDepositorInsertssgRNA + 2XPP7
PCP-VP64 IRES mCherry
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDN80
Plasmid#61349PurposeLINuS-flex: modular LINuS construct enabling introduction of any protein encoding sequence, NES and NLS. The sequence of interest replaces the LexA DBD-MBP-VP64 fragment.DepositorTypeEmpty backboneUseTagsAsLOV2 wt and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHAGE DN Cullin1
Plasmid#41911DepositorInsertCUL1 (CUL1 Human)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceApril 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDB22
Plasmid#61342PurposeMammalian expression of mCherry fused to LINuS, a light-inducible nuclear localization signal (cMycP1A NLS/PKIt NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-cMyc1A NLS and PKIt NESExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only
