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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,403 results for: puc

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Showing: 3781 - 3800 of 5403 results

  4. pIK198

    Plasmid
    #65629
    Purpose
    "flipped and extended" sgRNA backbone (Chen et al., 2013) following a C. elegans U6 promoter (Friedland et al., 2013). For Gibson cloning locus-specific sgRNA sequences.
    Depositor
    Iskra Katic
    Article
    Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Worm
    Promoter
    U6
    Available Since
    June 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    65629-plasmid-map-sequence-id-111540

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  5. pZH511

    Plasmid
    #102665
    Purpose
    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    102665-plasmid-map-sequence-id-199381

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  6. pIEforRed

    Plasmid
    #185892
    Purpose
    In vivo gene amplification (HapAmp) of EforRed expression module at RPL25 locus
    Depositor
    Claudia Vickers
    Article
    Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:
    Insert
    PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>EforRed>TPGK1-PBTS1> RPL25(partial; Arm2)
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185892-plasmid-map-sequence-id-370865

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  7. pTS2163

    Plasmid
    #169632
    Purpose
    Tier-3 vector for CRISPR-mediated stable integration into the AAVS1 locus of the tetON-system (OTetO7-PCMVmin-SEAP-p2A-iRFP670-pA::PmPGK1-rtTA-pA::A3-pA)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    OTetO7-PCMVmin-SEAP-iRFP670::PmPGK1-rtTA-pA::A3
    Expression
    Mammalian
    Promoter
    tetO7-PhCMVmin / PPGK
    Available Since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    169632-plasmid-map-sequence-id-366785

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Showing: 3781 - 3800 of 5403 results