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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,310 results for: LEA

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Showing: 3801 - 3820 of 12310 results

  1. Kaede-Nucleus-9

    Plasmid
    #57319
    Purpose
    Localization: Nucleus, Excitation: 508 / 572, Emission: 518 / 580
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Tags
    Kaede-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  4. SETD3

    Plasmid
    #32873
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SETD3 (SETD3 Human)
    Tags
    His tag (with TEV cleavage)
    Expression
    Bacterial
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. USP15

    Plasmid
    #28282
    Depositor
    Cheryl Arrowsmith
    Insert
    USP15 (USP15 Human)
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    Contains dusp and Ubl domains (residues 6-223).
    Available since
    April 14, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. SpCas9-NRCH-ABE8e(V106W)

    Plasmid
    #198556
    Purpose
    Expresses human codon-optimized SpCas9-NRCH-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8e(V106W)-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NRCH-ABE8e(V106W)
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198556-plasmid-map-sequence-id-388914

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  13. nLuc_AP10_PPVs_P9mS

    Plasmid
    #119302
    Purpose
    Expresses N-terminus of split luciferase fused to antiparallel coiled-coil AP10 connected with autoinhibitory coil P9mS and PPVs cleavage site in antiparallel harpin linker/for SPOC logic
    Depositor
    Roman Jerala
    Publication
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    split nLuc fused to antiparallel Coiled-coil AP10 connected with P9mS via PPV cleavable linker
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    119302-plasmid-map-sequence-id-232037

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  16. pcDNA3.1 SP-His-mCherry-HRP-VhHGFP

    Plasmid
    #112157
    Purpose
    Expression and secretion of anti-GFP FLIPPER-bodies, small protein probes for correlated microscopy. FLIPPER-bodies are the fusion of a nanobody with a fluorescent protein and a peroxidase.
    Depositor
    Ben Giepmans
    Publication
    de Beer et al Histochem Cell Biol. 2018 Mar;149(3):261
    Insert
    anti-GFP FLIPPER-body
    Tags
    His and Thrombin cleavage site
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112157-plasmid-map-sequence-id-213698

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  17. KLHL40 (KBTBD5A-c006)

    Plasmid
    #98233
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KBTBD5A (KLHL40 Human)
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98233-plasmid-map-sequence-id-193413

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  18. pMT-flk1-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80056
    Purpose
    Flk1/Kdrl promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    flk1
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80056-plasmid-map-sequence-id-151372

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Showing: 3801 - 3820 of 12310 results