We narrowed to 21,977 results for: Sis
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Plasmid#82031PurposePlasmid for constitutive or doxy-inducible expression of S664A+S679A+S745A human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInsertCtIP (RBBP8 Human)
UseTagsHAExpressionMammalianMutationNon phosphorylatable mutations on 3 of the main A…PromoterCMV-tetAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNOC_dfnCas12a_crRNA NC
Plasmid#176256PurposepNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and spacer sequence is replaced by the type IIS restriction site for endonuclease BaeI that can beDepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationD917A and E1006A mutations to inactivate the endo…PromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
sgSf3b1(T1)
Plasmid#90424Purposeinduces dsDNA break within mouse Sf3b1 gene for subsequent homology-directed recombination and coding sequence mutagenesis at codon 700DepositorInsertmus Sf3b1 gRNA (Sf3b1 Mouse)
UseCRISPR; Guiderna encoding for gene editingTagsExpressionMammalianMutationPromoterU6Available sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a-Nlux_crRNA NR1
Plasmid#176244PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1DepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterRibosomal subunitAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10::ZF1CACTA1_3xFLAG_TET1cd
Plasmid#106434PurposepUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that targets the CACTA1 promoter in arabidopsisDepositorInsertTET1 tet methylcytosine dioxygenase 1 (TET1 Human, Synthetic)
UseSynthetic BiologyTags3xFLAGExpressionPlantMutationPromoterAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBMN-neo-Flag-TNFR1 L380A Del229-244
Plasmid#44098DepositorInserthuman tumor necrosis factor receptor 1 (TNFRSF1A Human)
UseRetroviralTagsFlagExpressionMutationL380APromoterAvailable sinceApril 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
FKBP-beta2(616-951)-GFP Double
Plasmid#100729PurposeTo express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosisDepositorInsertAP2B1 (AP2B1 Human)
UseTagsFKBP and GFPExpressionMammalianMutationLong isoform, deletion of clathrin box motif and …PromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
FKBP-beta2(616-951)-GFP ∆CBM
Plasmid#100728PurposeTo express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosisDepositorInsertAP2B1 (AP2B1 Human)
UseTagsFKBP and GFPExpressionMammalianMutationLong isoform, deletion of clathrin box motifPromoterCMVAvailable sinceSept. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
FKBP-beta2(616-951)-GFP Y215A
Plasmid#100727PurposeTo express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosisDepositorInsertAP2B1 (AP2B1 Human)
UseTagsFKBP and GFPExpressionMammalianMutationLong isoform Y215A mutation (Y815A in full length)PromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWZLblasti-HA-MEK1-MEK5DD
Plasmid#53177PurposeExpresses HA tagged chimeric human MEK1 (nucleotides 1–201)-MEK5 (498–1344 with S311DT315D) from blasticidin resistance retroviral vectorDepositorInsertMEK1 (1-201)-MEK5 (498–1344) fusion (MAP2K1 Human)
UseRetroviralTagsHAExpressionMammalianMutationMEK1 (1-201) MEK5(498–1344 with S311D/T315D)PromoterAvailable sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-mEGFP-hCTNNA1_DM1-3_WT-ABD
Plasmid#234553PurposeCTNNA1 M-domain deletion mutantDepositorInsertmEGFP:hCTNNA1_DM-domain (CTNNA1 Human)
UseLentiviralTagsmEGFPExpressionMutationDeletion of aa 273-635PromoterCMVAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-mEGFP-hCTNNA1_L436P
Plasmid#234559PurposeCTNNA1 eye phenotype via forward genetic screenDepositorInsertmEGFP:hCTNNA1_L436P (CTNNA1 Human)
UseLentiviralTagsmEGFPExpressionMutationL436PPromoterCMVAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-mEGFP-hCTNNA1_DNii domain
Plasmid#234557PurposeCTNNA1 Nii-domain deletion mutantDepositorInsertmEGFP:hCTNNA1_DNii domain (CTNNA1 Human)
UseLentiviralTagsmEGFPExpressionMutationDeletion of aa ~165-265PromoterCMVAvailable sinceJune 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1-13X-gRNA1-2(AtUBI10)-MTAP-LUC
Plasmid#234370PurposeT-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAsDepositorInsertsNbGP41-sfGFP-VP64-GB1
Luciferase
dCas9-13XGP41
gRNA 1-1 and gRNA 1-2
UseSynthetic BiologyTagsGB1, GP41, and sfGFPExpressionPlantMutationPromoterArabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…Available sinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1-24X-gRNA1-2(AtUBI10)-MTAP-LUC
Plasmid#234372PurposeT-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAsDepositorInsertsNbGP41-sfGFP-VP64-GB1
Luciferase
dCas9-24XGP41
gRNA 1-1 and gRNA 1-2
UseSynthetic BiologyTagsGB1, GP41, and sfGFPExpressionPlantMutationPromoterArabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…Available sinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMVP-SEC24C
Plasmid#231559PurposepMVP expression vector for human SEC24C (wild type, closed, resistant to sgSEC24C #1 and #2) (Blasticidin selection marker)DepositorInsertSEC24C (SEC24C Human)
UseLentiviralTagsV5ExpressionMammalianMutationsilent PAM mutations (G67 (GGG>GGA) and A115 (…PromoterCMVAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ E4F1 degron(220-242)-GFP-IRES-mCherry
Plasmid#231007PurposeProtein stability reporter construct for E4F1 consisting of aa 220-242 for transient overexpression in mammalian cells.DepositorInsertE4F1 aa220-242 (E4F1 Human)
UseTagsAcGFP1ExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CAPN7-C290S-mCherry
Plasmid#193047PurposeLentiviral expression vector for stable integration of inducible, siRNA-resistant CAPN7 with C290S (proteolysis inactivating mutation) with C-terminal mCherry tag.DepositorInsertCAPN7 (CAPN7 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationC290S, siRNA resistant to: GCACCCAUACCUUUACAUUPromoterTight TREAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
hTph2p-Luc
Plasmid#223526PurposeFirefly-luciferase reporter expression driven by human Tph2 promoterDepositorInsertTph2 promoter (TPH2 Human)
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationPromoterhTph2 promoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-mCherry-KATNA1, V55D
Plasmid#180651PurposeLentiviral expression vector for KATNA1. Used for generating cell lines. Has N-terminal mCherry tag. Has V55D mutation. Dox-inducible. Internal ID: WISP20-48.DepositorInsertKATNA1 (KATNA1 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationsiRNA resistant to: GGACAGCACUCCCUUGAAAPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only