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nLYN267-gs10-NLS-VPR

Plasmid

#188265

Purpose

Plasmid ensures constitutive expression of the N-terminal fragment of split LYN kinase domain (aa 268-512), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

Depositor

Roman Jerala

Article

Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

Insert

nLYN267 (LYN Human)

Expression

Mammalian

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST47-SARA-GFP

Plasmid

#67410

Purpose

Mammalian expression of hSara with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
ELK3_pet28a

Plasmid

#131651

Purpose

Bacterial expression of ETS gene ELK3

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELK3 (ELK3 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_MESP1_P2A_Hygro_Barcode

Plasmid

#120459

Purpose

Barcoded lentiviral vector to express MESP1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MESP1 (MESP1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-IFT43

Plasmid

#218735

Purpose

Expresses N-terminally EGFP-tagged IFT43 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

Insert

IFT43 (IFT43 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF1712

Plasmid

#141869

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

DPRX (DPRX Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF0473

Plasmid

#142637

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SP140 (SP140 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-SARA-no stop

Plasmid

#67347

Purpose

hSara without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Use

Gateway entry vector

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pRK5-myc-Miro1 S432N

Plasmid

#47893

Purpose

Expresses myc tagged Miro1 S432N dominant negative mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro1 S432N (RHOT1 Human)

Tags

myc

Expression

Mammalian

Mutation

S432N, dominant negative mutant

Promoter

CMV

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pQTEV-MAP1LC3A

Plasmid

#34824

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

microtubule-associated protein 1 light chain 3 alpha (MAP1LC3A Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pDSHA-SARA-Myc

Plasmid

#67368

Purpose

Mammalian expression of hSara with C-term Myc tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pUDE731

Plasmid

#103008

Purpose

S. cerevisiae Episomal plasmid harboring Fncpf1 under the control of TDH3p

Depositor

Jean-Marc Daran

Article

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

Fncpf1

Tags

3HA and NLS

Expression

Yeast

Mutation

human codon optimized

Promoter

TEF1

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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TFORF1564

Plasmid

#143521

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF682 (ZNF682 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLPC-Flag-CHES1L

Plasmid

#51836

Purpose

encodes the long isoform of human CHES1 and a Flag tag

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

CHES1 (FOXN3 Human)

Use

Retroviral

Tags

Flag

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-mApg5(K130R)

Plasmid

#22959

Depositor

Noboru Mizushima

Article

Mizushima et al J Cell Biol. 2001 Feb 19. 152(4):657-68.

Insert

autophagy related genes 5 (Atg5 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

130 Lysine to Arginine

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3_DRD1-NTEV-TCS-GV-2xHA

Plasmid

#194358

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

DRD1-NTEV-TCS-GV-2xHA (DRD1 Synthetic, Human)

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC6A2

Plasmid

#131891

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Sijben et al Sci Rep. 2021 Jun 10;11(1):12290. doi: 1

Insert

SLC6A2 (SLC6A2 Human)

Expression

Mammalian

Available Since

Oct. 9, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pRetroSuper shAngptl4 hygro

Plasmid

#19154

Depositor

Joan Massague

Article

Padua et al Cell. 2008 Apr 4. 133(1):66-77.

Insert

small hairpin RNA against angiopoietin-like 4 (ANGPTL4 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
pMSCV-PIG-Lin28b

Plasmid

#64795

Purpose

Expresses human Lin28b in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Lin28b (LIN28B Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pLJM5-mDPYD

Plasmid

#61614

Purpose

The mouse Dpyd was used to rescue the human DPYD hairpin affect on EMT

Depositor

David Sabatini

Article

Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi:

Insert

dihydropyrimidine dehydrogenase (Dpyd Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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