We narrowed to 4,454 results for: TIL

1. pSA2

   Plasmid

   #52262

   Purpose

   Bacterial expression of human 6His-tagged SUMO2 and SUMO-E1

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Inserts

   SUMO-2 (SUMO2 Human)
   SAE-2 (UBA2 Human)
   SAE-1 (SAE1 Human)
   

   Use

   Tags

   6His-tag

   Expression

   Bacterial

   Mutation

   C-terminal gly-gly

   Promoter

   T7 and rbs

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-VTKS2-Dreadd-hM3Dq-HA-P2A-tBFP

   Plasmid

   #178707

   Purpose

   AAV vector for Cre-dependent transgene expression of Dreadd-hM3Dq-HA-P2A-tBFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

   Depositor

   Jordane Dimidschstein

   Article

   Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

   Insert

   Dreadd-hM3Dq-HA-P2A-tBFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRetroX-PTuner DD-M.EcoGII-v5-Centromeric protein C

   Plasmid

   #122085

   Purpose

   Express M.EcoGII fused to CENPC with destabilization domain - inducible

   Depositor

   Laure Crabbe

   Article

   Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

   Insert

   DD-linker-M.EcoGII-V5-CENPC (CENPC Human, E.coli)
   

   Use

   Retroviral

   Tags

   V5 tag on Nter of CENPC

   Expression

   Bacterial and Mammalian

   Mutation

   L341F, R930L (see comments below)

   Promoter

   Available since

   March 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS-EF1-IRES-NEO-Control

   Plasmid

   #80757

   Purpose

   Transposon Reporter

   Depositor

   Alex Kentsis

   Article

   Henssen et al Nat Genet. 2017 May 15. doi: 10.1038/ng.

   Insert

   NeoR (neoR Synthetic)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   EF1-IRES from PB-EF1-IRES-NEO

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKK-RNAi-nucCHERRYmiR-EGFP-TEV

   Plasmid

   #105810

   Purpose

   Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal EGFP.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   RNAi; Flp-in competent

   Tags

   EGFP-TEV

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKK-RNAi-nucCHERRYmiR-FLAG-TEV

   Plasmid

   #105809

   Purpose

   Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal FLAG.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   RNAi; Flp-in competent

   Tags

   FLAG-TEV

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiCRISPRv2-NONO 40

   Plasmid

   #127653

   Purpose

   Knock-out of human NONO

   Depositor

   Nicolas Manel

   Article

   Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

   Insert

   NONO sgRNA (NONO Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiCRISPRv2-NONO 4

   Plasmid

   #127652

   Purpose

   Knock-out of human NONO

   Depositor

   Nicolas Manel

   Article

   Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

   Insert

   NONO sgRNA (NONO Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. iMb2-Control-Mosaic (SO273)

   Plasmid

   #99750

   Purpose

   Second generation Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different membrane localized fluorescent proteins

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   Mb2YFP-2A, Mb2Tomato-2A, Mb2-HA-Tfp1
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSS02:cyt-Peredox-mCherry_DS

    Plasmid

    #161747

    Purpose

    cytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plants

    Depositor

    Markus Schwarzländer

    Article

    Steinbeck et al Plant Cell. 2020 Aug 13. pii: tpc.20.002

    Insert

    cyt-Peredox-mCherry_DS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    amino acid substitution D115S in first T-Rex doma…

    Promoter

    Ubiquitin 10

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPL001

    Plasmid

    #184750

    Purpose

    Big-IN positive control payload encoding EF1a-driven GFP-T2A-BSD.

    Depositor

    Jef Boeke

    Article

    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

    Insert

    pEF1a-eGFP-T2A-BSD-bGHpA
    

    Use

    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. PLL5.0-Anillin ShRNA-Cherry-Anillin deltaActin binding domain

    Plasmid

    #187276

    Purpose

    Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Actin binding domain

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    deltaActin binding domain

    Promoter

    U6, CMV

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pL452(hygro)-Sf3b1-K700K

    Plasmid

    #90426

    Purpose

    selectable HDR vector to introduce silent mutation (position 700) in mus Sf3b1 gene (for use with pL452-Sf3b1-K700E and sgSf3b1(T1) gRNA vectors enabling generation of hemizygous K700E mutation)

    Depositor

    Alex Minella

    Article

    Gupta et al Nucleic Acids Res. 2018 Nov 20. pii: 519

    Insert

    Sf3b1 - left and right homology arms (Sf3b1 Mouse)
    

    Use

    Homology-directed repair vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTriEx-PA-Rac1-C450A

    Plasmid

    #22025

    Purpose

    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
    

    Use

    Tags

    6X His

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV domain contains light-insensitive mutation C4…

    Promoter

    CMV, p10

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pGEX4T2-Ste7-EE

    Plasmid

    #52681

    Purpose

    expression of constitutively active Ste7 kinase in bacteria

    Depositor

    Benjamin Turk

    Article

    Mok et al Sci Signal. 2010 Feb 16;3(109):ra12. doi

    Insert

    Ste7 (STE7 Budding Yeast)
    

    Use

    Tags

    GST and Myc

    Expression

    Bacterial

    Mutation

    S359E/T363E constitutively active

    Promoter

    tac

    Available since

    April 30, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pTriEx-PA-Rac1-I539E

    Plasmid

    #22026

    Purpose

    Expression of photoactivatable Rac1 ('lit' state mutant)

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1-I539E (RAC1 Human, Avena sativa (oat))
    

    Use

    Tags

    6X His

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV domain contains 'lit state' mutatio…

    Promoter

    CMV, p10

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pRosa26-Acceptor (AG103)

    Plasmid

    #99632

    Purpose

    To insert the Tre-Tight or UAS ifgMosaic Donor vectors

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    Gt(Rosa)26Sor (Gt(ROSA)26Sor Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. PLL5.0-Anillin ShRNA-Cherry-Anillin deltaMyosin binding domain

    Plasmid

    #187275

    Purpose

    Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Myosin binding domain

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    deltaMyosin binding domain

    Promoter

    U6, CMV

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Tol2-pA-GI-TagRFP-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP

    Plasmid

    #135812

    Purpose

    This plasmid can be used to express independently eGFP and TagRFP from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.

    Depositor

    Nadia Mercader Huber

    Article

    Sanz-Morejon et al Cell Rep. 2019 Jul 30;28(5):1296-1306.e6

    Insert

    TagRFP
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    UAS

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMD9_2xUGI_3xHA

    Plasmid

    #136652

    Purpose

    Expresses St1BE4max-LMD9 in mammalian cells along with its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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