We narrowed to 48,845 results for: des.1
-
Plasmid#111861PurposeC-terminal GFP tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal GFP-Psc-StrepII tag.DepositorInsertHook3(1-552)-GFP (HOOK3 Human)
UseTagsGFP, Psc tag, and Strep tagExpressionInsectMutationOptimised for expression in SF9 cellsPromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLIB-FLAG(N)-CDK12deltaCTD(1-1082)
Plasmid#231735PurposeProtein expression in insect cellsDepositorInsertCDK12 (CDK12 Human)
UseTagsFlag-3CExpressionInsectMutationCDK12(1-1082)PromoterAvailable sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-eGFP:HA-mTREK-1(S131C/A286F)
Plasmid#224775PurposeMammalian expression vector for generating mouse TREK-1DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseTagsExpressionMammalianMutationS131C / A286FPromoterCMVAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1 (C159A/C219A/C365A/C399A)
Plasmid#224754PurposeIn vitro transcription for Oocyte expression of mTREK-1 (C159A/C219A/C365A/C399A)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationC159A/C219A/C365A/C399APromoterT7Available sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(1-280) C90A, T172C
Plasmid#217983Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationC90A, T172CPromoterT7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(1-280) A50C,C90A
Plasmid#217985Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationA50C, C90APromoterT7Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-SREBP-1 (A317-318; A341)
Plasmid#204194PurposeMammalian expression of SREBP-1 (Isoform 1)DepositorInsertSREBP-1 (SREBF1 Human)
UseTagsE-tag and T7x2ExpressionMammalianMutationGly to Ala 317,318,341PromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3- SREBP-1 (A317-318; L340)
Plasmid#204195PurposeMammalian expression of SREBP-1 (Isoform 1)DepositorInsertSREBP-1 (SREBF1 Human)
UseTagsE-tag and T7x2ExpressionMammalianMutationGly to Ala 317,318; Gly to Leu 340PromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(ZNF649_5-1)-PGKpuroBFP-W
Plasmid#212000PurposeExpress gRNA against ZNF649 with puro and BFPDepositorInsertsgRNA targeting ZNF649 (ZNF649 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterHuman U6 promoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(AAVS1-1)-PGKpuroBFP-W
Plasmid#211954PurposeExpress safe-cutter control gRNA with puro and BFPDepositorInsertsafe-cutter control sgRNA_AAVS1-1 (AAVS1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterHuman U6 promoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJRM919_WT aa 1-419 His POLI
Plasmid#201445PurposeLow copy vector for expressing the catalytic domain (amino acids 1-419) of human DNA polymerase iota in E. coli with an N-terminal 6x His tag. C-terminal truncation mutant of pJRM919_WT His POLIDepositorInsertDNA polymerase iota (POLI Human)
UseTags6x HisExpressionBacterialMutationExpresses amino acids 1-449 of DNA polymerase iot…PromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 AUP1 61-410
Plasmid#185334PurposeBacterial expression of AUP1 with GST fusion protein for binding assaysDepositorInsertAUP1 (AUP1 Human)
UseTagsGSTExpressionBacterialMutationaa 61-610 onlyPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Teton-puro-shFDPS #2
Plasmid#198760Purposeconditional knockdown of FDPSDepositorInsertshFDPS #2 (FDPS Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-HsUPF3b-iso2_145-470_K
Plasmid#146759PurposeBacterial Expression of HsUPF3biso2_145-470DepositorInsertHsUPF3biso2_145-470 (UPF3B Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
RPS4X sgRNA-1 lentiCRISPR v2 plasmid
Plasmid#192231Purposelentiviral vector expressing sgRNA-1 targeting eIF3 binding site of RPS4X 5'UTRDepositorInsertsgRNA-1 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-16-1-3p
Plasmid#103270PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-16-1-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-16-1-3p target (MIR16-1 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-138-1-3p
Plasmid#103232PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-138-1-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-138-1-3p target (MIR138-1 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-129-1-3p
Plasmid#103205PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-129-1-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-129-1-3p target (MIR129-1 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-125b-1-3p
Plasmid#103189PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-125b-1-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-125b-1-3p target (MIR125B1 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-128-1-5p
Plasmid#103198PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-128-1-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-128-1-5p target (MIR128-1 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only