We narrowed to 8,864 results for: plasmid dna
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Plasmid#66091Purposeco-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2DepositorInsertsgRNA for APs4
UseCRISPRExpressionWormPromoterU6Available SinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
AP332-3
Plasmid#66090Purposeco-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2DepositorInsertsgRNA for APs4
UseCRISPRExpressionWormPromoterU6Available SinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
AP332-2
Plasmid#66089Purposeco-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2DepositorInsertsgRNA for APs4
UseCRISPRExpressionWormPromoterU6Available SinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
AP303-3
Plasmid#66087Purposeco-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2DepositorInsertsgRNA for APs1
UseCRISPRExpressionWormPromoterU6Available SinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-EGFP
Plasmid#71666PurposeExpression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Synthetic, Human, S. pyogenes)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-EGFPExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9PromoterCBhAvailable SinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-PuroR
Plasmid#71667PurposeExpression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Synthetic, Human, S. pyogenes)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-PuroRExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9PromoterCBhAvailable SinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
10TALE_Pmin_fLuc
Plasmid#160663PurposeExpression of the firefly luciferase reporter upon binding of a transcriptional activator to target sites. The plasmid contains 10 copies of target sites for the TALEN1257 DNA-binding domainDepositorInsert10TALE_Pmin_fLuc
UseLuciferaseExpressionMammalianPromoterminimalAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTTP1A
Plasmid#91721Purposeintegration-proficient plasmid vector for mycobacteria, contains the attP and int region from Tweety genomic DNADepositorTypeEmpty backboneUseIntegration-proficient plasmid vectors for mycoba…Available SinceMay 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-hCHD7-Kozak ATG 3' 3x Flag-bGH polyA
Plasmid#89459Purposeconstitutive expression of human CHD7 in mammalian cellsDepositorAvailable SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTTP1B
Plasmid#91723Purposeintegration-proficient plasmid vector for mycobacteria, contains the attP and int region from Tweety genomic DNADepositorTypeEmpty backboneUseIntegration-proficient plasmid vectors for mycoba…Available SinceMay 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-hCHD7-Kozak ATG 3' HA-bGH polyA
Plasmid#89460Purposeconstitutive expression of human CHD7 in mammalian cellsDepositorAvailable SinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-puro
Plasmid#21915Purpose3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralAvailable SinceAug. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
TLCV2
Plasmid#87360PurposeLentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.DepositorHas ServiceCloning Grade DNAInsertCas9-2A-eGFP
UseCRISPR and Lentiviral; Doxycycline inducible; egf…TagsCas9-T2A-eGFPExpressionMammalianPromoterTight TRE promoterAvailable SinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA-PGK-puromycin
Plasmid#51133Purposeexpresses sgRNA from the U6 promoter.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRExpressionMammalianPromoterU6Available SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2
Plasmid#75112Purposelenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6 and EF1AAvailable SinceMay 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
sgRNA(MS2) cloning backbone
Plasmid#61424PurposesgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRExpressionMammalianPromoterU6Available SinceDec. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL410_INS421
Plasmid#49057PurposeExpresses luciferase under control of human insulin gene promoterDepositorAvailable SinceOct. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-Tet1-CD
Plasmid#83340PurposeTo introduce dCas9-fused Tet1-CD and sgRNA2.0 systemDepositorInsertdCas9-Tet1-CD and sgRNA scaffold with 2xMS2 binding sites
UseCRISPRExpressionMammalianMutationD10A, H840APromoterU6, CBHAvailable SinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1 puro GFP shRNA
Plasmid#10675PurposeNegative control vector for pMKO.1 puro (Addgene plasmid #8452); Contains shRNA against GFP.DepositorInsertGFP shRNA
UseRNAi and RetroviralExpressionMammalianAvailable SinceJan. 6, 2006AvailabilityAcademic Institutions and Nonprofits only -
145-pGL3ctrl-3' UTR
Plasmid#28175DepositorInsertLuciferase flanked by tp53 UTRs (TP53 Human)
UseLuciferaseExpressionMammalianMutationNote that insert length is based on sum of the 2 …Available SinceMarch 15, 2011AvailabilityAcademic Institutions and Nonprofits only