We narrowed to 4,489 results for: TIL;

1. iMb2-Control-Mosaic (SO273)

   Plasmid

   #99750

   Purpose

   Second generation Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different membrane localized fluorescent proteins

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   Mb2YFP-2A, Mb2Tomato-2A, Mb2-HA-Tfp1
   

   Use

   Cre/Lox and Mouse Targeting

   Expression

   Mammalian

   Available Since

   Oct. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKK-RNAi-nucCHERRYmiR-EGFP-TEV

   Plasmid

   #105810

   Purpose

   Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal EGFP.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   RNAi; Flp-in competent

   Tags

   EGFP-TEV

   Expression

   Mammalian

   Available Since

   March 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKK-RNAi-nucCHERRYmiR-FLAG-TEV

   Plasmid

   #105809

   Purpose

   Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal FLAG.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   RNAi; Flp-in competent

   Tags

   FLAG-TEV

   Expression

   Mammalian

   Available Since

   Feb. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSS02:cyt-Peredox-mCherry_DS

   Plasmid

   #161747

   Purpose

   cytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plants

   Depositor

   Markus Schwarzländer

   Article

   Steinbeck et al Plant Cell. 2020 Aug 13. pii: tpc.20.002

   Insert

   cyt-Peredox-mCherry_DS
   

   Expression

   Plant

   Mutation

   amino acid substitution D115S in first T-Rex doma…

   Promoter

   Ubiquitin 10

   Available Since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPL001

   Plasmid

   #184750

   Purpose

   Big-IN positive control payload encoding EF1a-driven GFP-T2A-BSD.

   Depositor

   Jef Boeke

   Article

   Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

   Insert

   pEF1a-eGFP-T2A-BSD-bGHpA
   

   Use

   Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTriEx-PA-Rac1-C450A

   Plasmid

   #22025

   Purpose

   Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   PA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
   

   Tags

   6X His

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   LOV domain contains light-insensitive mutation C4…

   Promoter

   CMV, p10

   Available Since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pTriEx-PA-Rac1-I539E

   Plasmid

   #22026

   Purpose

   Expression of photoactivatable Rac1 ('lit' state mutant)

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   PA-Rac1-I539E (RAC1 Avena sativa (oat), Human)
   

   Tags

   6X His

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   LOV domain contains 'lit state' mutatio…

   Promoter

   CMV, p10

   Available Since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

8. PLL5.0-Anillin ShRNA-Cherry-Anillin deltaActin binding domain

   Plasmid

   #187276

   Purpose

   Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Actin binding domain

   Depositor

   Alpha Yap

   Article

   Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

   Insert

   Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Mutation

   deltaActin binding domain

   Promoter

   U6, CMV

   Available Since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pL452(hygro)-Sf3b1-K700K

   Plasmid

   #90426

   Purpose

   selectable HDR vector to introduce silent mutation (position 700) in mus Sf3b1 gene (for use with pL452-Sf3b1-K700E and sgSf3b1(T1) gRNA vectors enabling generation of hemizygous K700E mutation)

   Depositor

   Alex Minella

   Article

   Gupta et al Nucleic Acids Res. 2018 Nov 20. pii: 519

   Insert

   Sf3b1 - left and right homology arms (Sf3b1 Mouse)
   

   Use

   Homology-directed repair vector

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX4T2-Ste7-EE

    Plasmid

    #52681

    Purpose

    expression of constitutively active Ste7 kinase in bacteria

    Depositor

    Benjamin Turk

    Article

    Mok et al Sci Signal. 2010 Feb 16;3(109):ra12. doi

    Insert

    Ste7 (STE7 Budding Yeast)
    

    Tags

    GST and Myc

    Expression

    Bacterial

    Mutation

    S359E/T363E constitutively active

    Promoter

    tac

    Available Since

    April 30, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pRosa26-Acceptor (AG103)

    Plasmid

    #99632

    Purpose

    To insert the Tre-Tight or UAS ifgMosaic Donor vectors

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    Gt(Rosa)26Sor (Gt(ROSA)26Sor Mouse)
    

    Use

    Mouse Targeting

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tol2-pA-GI-TagRFP-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP

    Plasmid

    #135812

    Purpose

    This plasmid can be used to express independently eGFP and TagRFP from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.

    Depositor

    Nadia Mercader Huber

    Article

    Sanz-Morejon et al Cell Rep. 2019 Jul 30;28(5):1296-1306.e6

    Insert

    TagRFP
    

    Use

    Zebrafish

    Promoter

    UAS

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PLL5.0-Anillin ShRNA-Cherry-Anillin deltaMyosin binding domain

    Plasmid

    #187275

    Purpose

    Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Myosin binding domain

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Mutation

    deltaMyosin binding domain

    Promoter

    U6, CMV

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTriEx-mVenus-PA-Rac1-C450A

    Plasmid

    #22021

    Purpose

    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
    

    Tags

    6X His and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV domain contains the light-insensitive C450A m…

    Promoter

    CMV, p10

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pSA3

    Plasmid

    #52263

    Purpose

    Bacterial expression of human 6His-tagged SUMO3 and SUMO-E1

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Inserts

    SUMO-3 (SUMO3 Human)
    SAE-2 (UBA2 Human)
    SAE-1 (SAE1 Human)
    

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    C-terminal gly-gly

    Promoter

    T7 and rbs

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMD9_2xUGI_3xHA

    Plasmid

    #136652

    Purpose

    Expresses St1BE4max-LMD9 in mammalian cells along with its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. nCas9-UGI-NLS-P2A-EGFP (pJUL1001)

    Plasmid

    #123611

    Purpose

    CAG promoter expression plasmid for hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (BE3 control without rAPOBEC1).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

    Insert

    nCas9-UGI-NLS-P2A-EGFP
    

    Expression

    Mammalian

    Mutation

    D10A in SpCas9

    Promoter

    CAG

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-CARMA2sh-E138A

    Plasmid

    #97068

    Purpose

    Expresses Psoriasis-Linked mutant CARD14/CARMA2 in mammalian cells

    Depositor

    Pasquale Vito

    Article

    Scudiero et al Cell Death Dis. 2017 Feb 23;8(2):e2627.

    Insert

    CARD14 isoform 3 (CARD14 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Glutamic Acid 138 to Alanine

    Promoter

    CMV

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTRIP-SFFV-mtagBFP-2A STING V155M 342STOP

    Plasmid

    #102594

    Purpose

    Lentivector to express STING V155M delta 342 and mTagBFP

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    V155M delta 342

    Promoter

    SFFV

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOTTC1440 - pAAV CMV-IE Nuc-iRFP-2A-Flpo

    Plasmid

    #112684

    Purpose

    An AAV vector that expresses a nuclear-localized iRFP713 reporter and optimized Flp recombinase

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    iRFP713
    

    Use

    AAV

    Tags

    2A-Flpo and Nuclear localization signal

    Promoter

    CMV-IE

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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