182,008 results
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Plasmid#1730PurposeThis is a 3rd generation lentiviral transfer vector (slightly lower titer, highest expression level) from Nolan lab's FELIX vector systemDepositorTypeEmpty backboneUseLentiviralAvailable sinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by
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TFORF3550
Plasmid#145026PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.DepositorInsertmCherry
UseLentiviralExpressionMammalianPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
psfaMs-Tn5
Plasmid#183637PurposeExpresses anti-mouse nanobody fused with Tn5DepositorInsertanti mouse secondary nanobody fused to Tn5
Tagsanti-mouse nanobodyExpressionBacterialAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MMLVgag-3xNES-ABE8e-NG
Plasmid#181754PurposeMMLV-Gag fused to 3xNES-ABE8e-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMMLVgag-3xNES-ABE8e-NG
TagsFlagExpressionMammalianPromoterCMVAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SepRS(2)/pSertRNA(B4)/EF-Sep
Plasmid#173897PurposeThe plasmid contains an orthogonal aminoacyl-tRNA synthetase/tRNACUA pair, which directs the efficient incorporation of phosphoserine (pSer) into recombinant proteins in Escherichia coli.DepositorInsertsSepRS(2)
pSertRNA
EF-Sep
UseSynthetic BiologyExpressionBacterialAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-Hs.cargo(Cas9)
Plasmid#174864PurposeExpresses human Peg10 cargoRNA encoding SpCas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9 flanked by HsPeg10 UTRs
TagsHA-SV40 NLS and SV40 NLSExpressionMammalianPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV_hSyn1-SIO-stGtACR2-FusionRed (AAV Retrograde)
Viral prep#105677-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV_hSyn1-SIO-stGtACR2-FusionRed (#105677). In addition to the viral particles, you will also receive purified pAAV_hSyn1-SIO-stGtACR2-FusionRed plasmid DNA. Synapsin-driven, Cre-dependent, soma-targeted anion-conducting channelrhodopsin fused to FusionRed for optogenetic inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsFusionRed (Cre-dependent)Available sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-SARS-CoV-2-nsp1-2xStrep-IRES-Puro
Plasmid#141367PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-nsp1 (ORF1ab SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
mNeonGreen-mTurquoise2
Plasmid#98886PurposeProduces a fusion between mTurquoise2 and mNeonGreen. It can be used as a positive control for FRETDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmNeonGreen-mTurquoise2
ExpressionMammalianPromoterCMVAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PLJR962
Plasmid#115162PurposeSth1 dCas9 TetR and KanR L5 Int attP for M. smegmatisDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsSth1 sgRNA scaffold
Sth1 dCas9
TetR
L5 attP
L5 Int
KanR
UseUnspecifiedAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKM461
Plasmid#108320PurposeFor performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase, and contains SacBDepositorInsertsChe9C RecT
Bxb1 Integrase
Tet Repressor
SacRB
ExpressionBacterialAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLentiPGK Blast DEST ERKKTRmRuby2
Plasmid#90231PurposeLentiviral vector to express ERK KTR mRuby2 under PGK promoter (With Blasticidin Resistance)DepositorInsertERK Kinase Translocation Reporter
UseLentiviralTagsmRuby2ExpressionMammalianPromoterPGKAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PPRE-pNL1.3
Plasmid#84394PurposeSecreted Luciferase reporter for PPARgamma activityDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPPARG response element (PPARG Human)
TagssecNlucExpressionMammalianPromoterPPREAvailable sinceJan. 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG mito-GCaMP5G
Plasmid#105009Purposemitochondrial calcium sensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGCaMP5G
ExpressionMammalianPromoterCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-Antares2 c-myc
Plasmid#100027PurposeA BRET based CyOFP-teLuc-CyOFP fusion to further red-shift bioluminescenceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAntares2
Tagsc-mycExpressionMammalianPromoterCMVAvailable sinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmScarlet-H_C1
Plasmid#85043PurposeMammalian expression of mScarlet-HDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmScarlet-H
ExpressionMammalianPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB-TAC-ERP2
Plasmid#80478PurposeAll-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and puromycin resistance)DepositorTypeEmpty backboneUsePiggybac transposonExpressionMammalianAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJL-TRBO
Plasmid#80082PurposeAn improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence.DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FUGW-d2GFP-ZEB1 3'UTR
Plasmid#79601PurposeFUGW lentiviral vector with constitutively expressed destabilized GFP (d2GFP) regulated by the human ZEB1 3'UTR. When used in combination with Ecad-RFP, comprises the Z-cad dual sensor.DepositorPublicationInsertd2GFP-Zeb1 3'UTR
UseLentiviralAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CRISPRainbow Kit for Multiplex Labeling
Plasmid kit#1000000079PurposePlasmids using dCas9 and gRNA scaffolds that bind fluorescent proteins to label multiple chromosomal loci.DepositorApplicationGenome EditingVector typeLentiviral, Mammalian ExpressionEditing typeCRISPRAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only