We narrowed to 20,096 results for: Cre

1. pXGFPC-5 Pxyl-dcas9 (Sth3)

   Plasmid

   #133317

   Purpose

   expresses dcas9 (Streptococcus thermophilus #3); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xyl locus in Caulobacter crescentus; tetracycline resistance

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   dcas9 (Streptococcus thermophilus #3)
   

   Use

   Unspecified

   Promoter

   xylose

   Available Since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF-pIgH-ESCargo*

   Plasmid

   #140159

   Purpose

   Expresses a regulatable secretory cargo (without an ER export signal) for mammalian cells

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   piGH-DsRed-Express2-FKBP(LV)(C22V)
   

   Tags

   ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_ctrA

   Plasmid

   #133339

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_ctrA
   

   Use

   CRISPR

   Promoter

   constitutive

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF-pIgH-ESCargo(FTV,Orange)

   Plasmid

   #140161

   Purpose

   Expresses a regulatable secretory cargo for mammalian cells

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   piGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)
   

   Tags

   ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVCERC-5 Pvan-dcas9 (Sth3)

   Plasmid

   #133319

   Purpose

   expresses dcas9 from Streptococcus thermophilus #3; induced with vanillate; for homologous recombination at the vanillate locus in Caulobacter crescentus; tetracycline resistance

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   dcas9 (Streptococcus thermophilus #3)
   

   Use

   Unspecified

   Promoter

   vanillate

   Available Since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICH47732:PtFCP:ShBle

   Plasmid

   #104893

   Purpose

   Expresses ShBle with FCP promoter/terminator, Golden Gate Assembly, for Phaeodactylum transformation.

   Depositor

   Thomas Mock

   Article

   Graff van Creveld et al Front Microbiol. 2021 Sep 8;12:688199. d

   Insert

   ShBle
   

   Use

   Phaeodactylum; golden gate assembly

   Tags

   PtFCP promoter and terminator

   Available Since

   Dec. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXGFPC-5 Pxyl-dcas9 (Spa)

   Plasmid

   #133318

   Purpose

   expresses dcas9 (Streptococcus pasteurianus); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   dcas9 (Streptococcus pasteurianus)
   

   Use

   Unspecified

   Promoter

   xylose

   Available Since

   Feb. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEG BacMam C term 3C eGFP myc

   Plasmid

   #160685

   Purpose

   Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

   Depositor

   Eric Gouaux

   Article

   Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

   Type

   Empty backbone

   Tags

   His8 eGFP 3C

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBXMCS-2 Pconstitutive-sgRNA(Spa)_ctrA

   Plasmid

   #133342

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_ctrA
   

   Use

   CRISPR

   Promoter

   constitutive

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA1

    Plasmid

    #133340

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the non-template strand in Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_gcrA
    

    Use

    CRISPR

    Promoter

    constitutive

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBXMCS-2 Pconstitutive-sgRNA(Spy)_ctrA

    Plasmid

    #133334

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus pyogenes with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_ctrA
    

    Use

    CRISPR

    Promoter

    constitutive

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEG BacMam N term myc eGFP 3C

    Plasmid

    #160682

    Purpose

    Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

    Depositor

    Eric Gouaux

    Article

    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

    Type

    Empty backbone

    Tags

    myc eGFP 3C

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAGM4723-Del

    Plasmid

    #112207

    Purpose

    Level 2 cloning vector, for golden gate cloning for diatoms. All the Agrobacterium T-DNA delivery were deleted.

    Depositor

    Thomas Mock

    Article

    Graff van Creveld et al Elife. 2023 Jun 6;12:e84392. doi: 10.755

    Type

    Empty backbone

    Use

    Golden gate assembly

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA

    Plasmid

    #133344

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets cpaA gene's promoter from Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_cpaA
    

    Use

    CRISPR

    Promoter

    constitutive

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICH47742:PtFCP:Cas9YFP

    Plasmid

    #104894

    Purpose

    Cas9, with FCP promoter/terminator, Golden Gate Assembly, for Phaeodactylum transformation

    Depositor

    Thomas Mock

    Article

    Graff van Creveld et al Front Microbiol. 2021 Sep 8;12:688199. d

    Insert

    Cas9
    

    Use

    CRISPR; ; golden gate assembly

    Tags

    YFP

    Available Since

    July 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUASt-ssBiP-ESCargo*

    Plasmid

    #140165

    Purpose

    Expresses a regulatable secretory cargo (without an ER export signal) for Drosophila melanogaster

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    ssBip-DsRed-Express2-FKBP(LV)(C22V)
    

    Expression

    Insect

    Promoter

    hs70

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. psecBFP2

    Plasmid

    #62230

    Purpose

    encodes an inert blue fluorescent protein for use in oxidizing cellular environments including the periplasm and mammalian secretory pathway.

    Depositor

    Erik Snapp

    Article

    Costantini et al Biochem Biophys Res Commun. 2013 Jan 18;

    Insert

    secBFP2
    

    Tags

    secbfp2

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pCVD447

    Plasmid

    #78569

    Purpose

    E. coli E2348/69 LEE region (Type 3 Secretion System) cloned into pBluescriptKS.

    Depositor

    James Kaper

    Article

    Jarvis et al Proc Natl Acad Sci U S A. 1995 Aug 15;92

    Insert

    lee operon
    

    Use

    Cloning vector

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

19. SP_H3

    Plasmid

    #228189

    Purpose

    Plasmid contains the secretion signal peptide sequence of the yeast CIS3 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    sp_P47001_CIS3_YEAST:0-21
    

    Use

    Synthetic Biology

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SP_H4

    Plasmid

    #228190

    Purpose

    Plasmid contains the secretion signal peptide sequence of the human CCL8 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    sp_P80075_CCL8_HUMAN:0-23
    

    Use

    Synthetic Biology

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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