We narrowed to 23,801 results for: Sis

1. pUC57-4eCOL2A1

   Plasmid

   #97211

   Purpose

   For cloning of a COL2A1-based, chondrogenesis-responsive promoter, and subcloning this insert into new vectors

   Depositor

   Ryan Porter

   Article

   De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d

   Insert

   4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
   

   Use

   Vector is designed for cloning and generation of …

   Tags

   Expression

   Mutation

   Promoter

   COL2A1 regulatory elements

   Available Since

   Oct. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCold I-Hero11

   Plasmid

   #187928

   Purpose

   Bacterial expression of heat-resistant obscure (Hero) protein Hero11

   Depositor

   Yukihide Tomari

   Article

   Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

   Insert

   Hero11 (C19orf53 Human)
   

   Use

   Tags

   Flag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-FLEXfrt-SaCas9-U6-sgGria1

   Plasmid

   #124867

   Purpose

   Mutagenesis of Gria1

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gria1 (Gria1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. FKBP-beta3(702-1094)-GFP

   Plasmid

   #100734

   Purpose

   To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   AP3B1 (AP3B1 Human)
   

   Use

   Tags

   FKBP and GFP

   Expression

   Mammalian

   Mutation

   appendage and ear only

   Promoter

   CMV

   Available Since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-FLEXfrt-SaCas9-U6-sgGria2

   Plasmid

   #124868

   Purpose

   Mutagenesis of Gria2

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gria2 (Gria2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. FKBP-beta2(616-951)-mRuby2

   Plasmid

   #100730

   Purpose

   To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   AP2B1 (AP2B1 Human)
   

   Use

   Tags

   FKBP and mRuby2

   Expression

   Mammalian

   Mutation

   Long isoform

   Promoter

   CMV

   Available Since

   Sept. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-FLEX-SaCas9-U6-sgGria3

   Plasmid

   #124855

   Purpose

   Mutagenesis of Gria3

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gria3 (Gria3 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. CD8-pTagRFP657-LOVpep (T406A, T407A, I532A)

   Plasmid

   #100742

   Purpose

   To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   CD8A (CD8A Human)
   

   Use

   Tags

   LOVpep and tagRFP657

   Expression

   Mammalian

   Mutation

   Truncated

   Promoter

   CMV

   Available Since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-FLEXfrt-SaCas9-U6-sgGrin1

   Plasmid

   #124865

   Purpose

   Mutagenesis of Grin1

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Grin1 (Grin1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiCRISPR v2-sgBAX-2

    Plasmid

    #210130

    Purpose

    knock out BAX in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

    Insert

    Apoptosis regulator BAX (BAX Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-mCherry-KATNA1

    Plasmid

    #180650

    Purpose

    Lentiviral expression vector for KATNA1. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-47.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    KATNA1 (KATNA1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    siRNA resistant to: GGACAGCACUCCCUUGAAA

    Promoter

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-ybbr-HIS-ELP-ddFLN4-I27-XMod-Doc (wild type)

    Plasmid

    #153442

    Purpose

    E. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 and I27 fingerprint domains. This construct was designed for AFM measurements.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.XDocB
    

    Use

    Tags

    3xELP linker, 6xHis, Titin I27, ddFLN4 fingerprin…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCold I-Hero13

    Plasmid

    #187929

    Purpose

    Bacterial expression of heat-resistant obscure (Hero) protein Hero13

    Depositor

    Yukihide Tomari

    Article

    Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

    Insert

    Hero13 (BEX3 Human)
    

    Use

    Tags

    Flag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWZLblasti-HA-MEK1 C121S

    Plasmid

    #53165

    Purpose

    Expresses HA tagged human MEK1 C121S from blasticidin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK1 (MAP2K1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C121S

    Promoter

    Available Since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

15. hHSF1 S303A

    Plasmid

    #118363

    Purpose

    This plasmid expresses human HSF1 S303A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

    Insert

    hHSF1 (HSF1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    S303A

    Promoter

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. MSCV-TAP(FLAG-HA) CYLD

    Plasmid

    #60031

    Purpose

    CYLD with N-terminal TAP-tag (HA,FLAG) and IRES-Puro to allow tagged protein to be maintained

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

    Insert

    Cylindromatosis (CYLD Human)
    

    Use

    Retroviral

    Tags

    FLAG, HA, and IRES Puro

    Expression

    Mammalian

    Mutation

    Promoter

    LTR-driven expression

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

17. CD8-dCherry-FRB

    Plasmid

    #100739

    Purpose

    To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis (dark mCherry K70N)

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    CD8A (CD8A Human)
    

    Use

    Tags

    FRB and dCherry

    Expression

    Mammalian

    Mutation

    Dark mCherry (K70N) non-fluorescent

    Promoter

    CMV

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMMP-NGFR:LMP1-IRES-mRFP

    Plasmid

    #36974

    Depositor

    Bill Sugden

    Article

    Pratt et al J Virol. 2012 Apr;86(8):4380-93. Epub 20

    Insert

    NGFR:LMP1 (NGFR, LMP-1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    NGFR:LMP1 chimeric receptor consists of aa 1–276 …

    Promoter

    CMV

    Available Since

    Aug. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKIF1C-2xFLAG

    Plasmid

    #130979

    Purpose

    Expression of KIF1C-2xFLAG in mammalian cells.

    Depositor

    Anne Straube

    Article

    Theisen et al Dev Cell. 2012 Dec 11;23(6):1153-66. doi

    Insert

    KIF1C-2xFLAG (KIF1C Human)
    

    Use

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    4 silent mutations that make this construct resis…

    Promoter

    CMV

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-FLEXfrt-SaCas9-U6-sgGria3

    Plasmid

    #124869

    Purpose

    Mutagenesis of Gria3

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gria3 (Gria3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge