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We narrowed to 17,299 results for: sgRNA

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Showing: 3901 - 3920 of 17299 results

  2. pspCas9-HF-tetra-com-vector

    Plasmid
    #132555
    Purpose
    Vector plasmid expressing high fidelity spCas9 (R691A) and sgRNA scaffold with com replacing the Tetraloop.
    Depositor
    Baisong Lu
    Publication
    Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    High fidelity Cas9
    sgRNA scaffold with com replacing the Tetraloop
    Expression
    Mammalian
    Available since
    March 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    132555-plasmid-map-sequence-id-259534

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  4. ATP1A1_G3_MTOR_Nick_Exon-44_Dual_sgRNA

    Plasmid
    #178096
    Purpose
    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick exon-44 sgRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Publication
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G3 + MTOR nick exon-44 sgRNAs
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178096-plasmid-map-sequence-id-350135

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  5. ATP1A1_G3_MTOR_Nick_Intron-45_Dual_sgRNA

    Plasmid
    #178099
    Purpose
    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick intron-45 sgRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Publication
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G3 + MTOR nick intron-45 sgRNAs
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178099-plasmid-map-sequence-id-350138

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  6. ATP1A1_G8_MTOR_Nick_Exon-44_Dual_sgRNA

    Plasmid
    #178105
    Purpose
    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick exon-44 sgRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Publication
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G8 + MTOR nick exon-44 sgRNAs
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178105-plasmid-map-sequence-id-350131

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  7. ATP1A1_G8_MTOR_Nick_Intron-45_Dual_sgRNA

    Plasmid
    #178106
    Purpose
    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick intron-45 sgRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Publication
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G8 + MTOR nick intron-45 sgRNAs
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178106-plasmid-map-sequence-id-350189

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  12. pX459-HypaCas9-mR2-TUBA1B_sgRNA

    Plasmid
    #183888
    Purpose
    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.
    Depositor
    Alisa Piekny
    Publication
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TUBA1B sgRNA spacer (TUBA1B Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    183888-plasmid-map-sequence-id-362425

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  16. MSP2279

    Plasmid
    #70706
    Purpose
    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    D10A and H557A in SaCas9
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70706-plasmid-map-sequence-id-127768

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  18. lentiCRISPR - Amplicon, BCR/ABL sgRNA 2

    Plasmid
    #70658
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone.
    Depositor
    David Sabatini
    Publication
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    March 28, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 3901 - 3920 of 17299 results