We narrowed to 7,663 results for: ski

1. pCDNA5/FRT_CMV_NES-Caprola_04-mEGFP

   Plasmid

   #194682

   Purpose

   CMV driven expression of the calcium recorder Caprola_04 in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_04-mEGFP
   

   Tags

   mEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV4a-SIRT2-Flag

   Plasmid

   #102623

   Purpose

   Expresses full length SIRT2 isoform 1 in mammalian cells

   Depositor

   Hening Lin

   Article

   Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

   Insert

   Sirtuin 2 (SIRT2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA5/FRT/TO_TOMM20_HaloTag9_T2A_EGFP

   Plasmid

   #169333

   Purpose

   Expression of HaloTag9 at the mitochondrial surface of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   TOMM20-HaloTag9 (TOMM20 Human)
   

   Tags

   HaloTag9

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA SF-HERC2 F6

   Plasmid

   #55789

   Purpose

   Expresses N-terminal 3xFLAG/STREP tagged HERC2 (4421-4834aa)

   Depositor

   David Chan

   Article

   Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

   Insert

   3xFLAG tagged HERC2 (4421-4834aa) (HERC2 Human)
   

   Tags

   3xFLAG and STREP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA pDEST40 Cas9-POLD3

   Plasmid

   #183194

   Purpose

   Expression clone

   Depositor

   Emma Haapaniemi

   Article

   Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

   Insert

   POLD3
   

   Use

   CRISPR

   Tags

   Cas9WT

   Expression

   Mammalian

   Mutation

   n/a

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hsyn-flex-dsRed-shvgat

   Plasmid

   #67845

   Purpose

   Expresses shRNA to the vesicular GABA transporter VGAT. Cre dependent expression by flex switch

   Depositor

   William Wisden

   Article

   Yu et al Neuron. 2015 Jun 17. pii: S0896-6273(15)

   Insert

   AAV-hsyn-flexed-dsRed-miR30-vgat2.shRNA
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and RNAi

   Expression

   Mammalian

   Promoter

   h-synapsin

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. His-MBP_G4G6 in pRSF

   Plasmid

   #188454

   Purpose

   Express in E. coli human gelsolin segments 4 through 6 with N-terminal 6xHis and MBP tags.

   Depositor

   Roberto Dominguez

   Article

   Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

   Insert

   G4G6 (GSN Human)
   

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. p3HM1Luc4

   Plasmid

   #236277

   Purpose

   Mammalian expression of human muscarinic M1 receptor; N-terminal HA tag, C-terminal Rluc8

   Depositor

   Vsevolod Gurevich

   Article

   Gimenez et al J Biol Chem. 2012 Aug 24;287(35):29495-5

   Insert

   muscarinic M1 receptor (CHRM1 Human)
   

   Tags

   3xHA and Rluc8

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-R-iLACCO1.1

   Plasmid

   #208026

   Purpose

   Biosensor for intracellular L-lactate

   Depositor

   Robert Campbell

   Article

   Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

   Insert

   R-iLACCO1.1
   

   Expression

   Mammalian

   Available Since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO.1-puro_CMV_NES-Caprola_06-mEGFP

    Plasmid

    #194693

    Purpose

    CMV driven expression of the calcium recorder Caprola_06 fused to mEGFP for neuronal expression through through lentivirus transduction

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_06-mEGFP
    

    Use

    Lentiviral

    Tags

    mEGFP

    Promoter

    CMV

    Available Since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1-puro_CMV_NES-Caprola_off-mEGFP

    Plasmid

    #194695

    Purpose

    CMV driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through lentivirus transduction

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_off-mEGFP
    

    Use

    Lentiviral

    Tags

    mEGFP

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEND-321_Cacnes_ori

    Plasmid

    #225603

    Purpose

    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a high-copy pBR322 E. coli ori

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEND-350_Cacnes_ori

    Plasmid

    #225605

    Purpose

    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. retro-gRNA-mRFP1

    Plasmid

    #112914

    Purpose

    Retrovirus expressing mRFP1 with BBSI cloning sites for sgRNA

    Depositor

    Christophe Benoist, Diane Mathis

    Article

    Magnuson et al Proc Natl Acad Sci U S A. 2018 Nov 6;115

    Insert

    mRFP1
    

    Use

    Retroviral

    Promoter

    EF1a

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. T/Brachyury-eGFP Rex Neo

    Plasmid

    #21222

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    Brachyury promoter
    

    Use

    Lentiviral

    Tags

    eGFP

    Available Since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET51b-His-TEV-SNAP-tag_fast

    Plasmid

    #167271

    Purpose

    Overexpression of the fast variant (E30R) of SNAP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAPf-tag
    

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    E30R

    Promoter

    T7

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. lentiCRISPRi(v1)-Blast

    Plasmid

    #170067

    Purpose

    Expresses dCas9 repressor KRAB-dCas9 and blasticidin resistance

    Depositor

    Neville Sanjana

    Article

    Morris et al Science. 2023 May 19;380(6646):eadh7699.

    Insert

    KRAB-dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    2A and 2xHA tag

    Expression

    Mammalian

    Promoter

    EFS-NS

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21a-C2-mKate

    Plasmid

    #208763

    Purpose

    To express C2-mKate in bacteria

    Depositor

    Urte Neniskyte

    Article

    Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

    Insert

    C2-mKate (Mfge8 Mouse)
    

    Tags

    mKate

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZS*12-MS2-GFP

    Plasmid

    #26245

    Depositor

    Philippe Cluzel

    Article

    Guet et al Nucleic Acids Res. 2008 Jul . 36(12):e73

    Insert

    MS2-GFP
    

    Expression

    Bacterial

    Mutation

    five amino acid linker (Ser, Gly, Gly, Gly, Gly) …

    Available Since

    Nov. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. W118-1_hTGM2-V5

    Plasmid

    #180413

    Purpose

    lentiviral transduction of human TGM2 transcript variant 2 (with a C-terminal V5 tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    TGM2 (TGM2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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