We narrowed to 19,259 results for: Tec;

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1M-Tm-BC2

Plasmid

#206943

Purpose

Mamamlian Expression of 1M-Tm-BC2Tag EMcapsulins

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

MmMT3, TmEnc

Tags

BC2

Expression

Mammalian

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pBK-VQR-BE3

Plasmid

#85171

Purpose

Expresses VQR-BE3 in mammalian cells

Depositor

David Liu

Article

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

VQR-BE3

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pShuttle

Plasmid

#192871

Purpose

Gateway compatible vector with BsaI cloning site

Depositor

Thorsten Stiewe

Article

Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

Type

Empty backbone

Use

Gateway-compatible cloning vector

Available Since

Dec. 12, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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EK0393 SFFV-mTagBFP2-SGc(EGFP.s2)cSG-mCherry (FLP-IN)

Plasmid

#191159

Purpose

Expression of a sensor for EGFP CDS in mammalian cells; mTagBFP2 marker, mCherry output

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mTagBFP:EGFP.s2:mCherry

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.4_1G11F2-3 light chain

Plasmid

#198254

Purpose

recombinant expression of light chain of monoclonal anti-AMP antibody 1G11F2-3

Depositor

Aymelt Itzen

Article

Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

Insert

light chain of monoclonal anti-AMP antibody 1G11F2-3 (mouse kappa)

Tags

IL10 signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pShuttle-CMV-iRFP

Plasmid

#31856

Purpose

a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

Depositor

Vladislav Verkhusha

Article

Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

Insert

iRFP

Use

Adenoviral

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…

Promoter

CMV

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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HBT-pcoCas9

Plasmid

#52254

Purpose

transient expression of pcoCas9 gene in plant cells

Depositor

Jen Sheen

Article

Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d

Insert

pcoCas9

Use

CRISPR; Plant expression

Tags

FLAG and NLS

Promoter

hybrid constitutive promoter 35SPPDK

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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pTXB1-TnY

Plasmid

#163975

Purpose

IPTG-inducible expression of Transposon TnY fused to Mxe Intein and Chitin-binding domain.

Depositor

Peter Smibert

Article

Liscovitch-Brauer et al Nat Biotechnol. 2021 Apr 29. pii: 10.103

Insert

TnY

Tags

Mxe intein - Chitin-binding domain

Expression

Bacterial

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pX-evoCas9

Plasmid

#107550

Purpose

Expresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cells

Depositor

Anna Cereseto

Article

Casini et al Nat Biotechnol. 2018 Jan 29. pii: nbt.40

Insert

Humanized S. pyogenes Cas9

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Mutation

M495V/Y515N/K526E/R661Q

Promoter

CBh

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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Cytosolic FAK biosensor

Plasmid

#78300

Purpose

FRET biosensor. Visualization of cytosolic FAK activity in live cells

Depositor

Yingxiao Wang

Article

Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

Insert

Cytosolic FAK biosensor

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2016

Availability

Academic Institutions and Nonprofits only

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FLAG-Foxo1(pCMV5)

Plasmid

#12148

Depositor

Domenico Accili

Article

Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

Insert

Foxo1 (Foxo1 Mouse)

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2007

Availability

Academic Institutions and Nonprofits only

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pGWB417

Plasmid

#74811

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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pY026

Plasmid

#84741

Purpose

Expresses huAsCpf1 and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Insert

huAsCpf1

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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EK0313 SFFV-mCherry-SG(s70587)SG-Cre (FLP-IN)

Plasmid

#191149

Purpose

Expression of Sensor 1 (for EK0208) in mammalian cells; mCherry marker, Cre output derived from a gift from prof. Liqun Luo

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:s70587:Cre

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_123

Plasmid

#96919

Purpose

for CRISPRi, lentiviral expression of dCas9-p300 2A BlastR.

Depositor

John Doench, David Root

Article

Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

Insert

dCas9

Use

Lentiviral

Tags

p300

Expression

Mammalian

Mutation

dCas9

Promoter

EF1a

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ExRai-AktAR

Plasmid

#118462

Purpose

cpGFP-based excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-AktAR

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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piGECI-N1

Plasmid

#160421

Purpose

Cytoplasmic expression of a near-infrared genetically encoded calcium indicator iGECI

Depositor

Vladislav Verkhusha

Article

Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

Insert

iGECI

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pBK-YEE-BE3

Plasmid

#85177

Purpose

Expresses YEE-BE3 in mammalian cells

Depositor

David Liu

Article

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

YEE-BE3

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pGWB515

Plasmid

#74857

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with 3xHA (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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PB/pHSP-tTA-TRE-tTA-pEF1α-PuroR

Plasmid

#206213

Purpose

PiggyBac-based transposon vector plasmid which encodes an expression unit of tetracycline-responsive transactivator under control of HSP promoter with transcriptional amplification

Depositor

Masamichi Kamihira

Article

Kitano et al Cytotechnology. 2021 Jun;73(3):353-362.

Insert

tetracycline-responsive transactivator

Expression

Mammalian

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro cTnT FUCCI

Plasmid

#136935

Purpose

donor plasmid for cardiac-specific FUCCI expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

Insert

Troponin T promoter

Use

Synthetic Biology

Expression

Mammalian

Promoter

cTnT

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hAsCpf1(TYCV) (pY210)

Plasmid

#89351

Purpose

Expresses humanized AsCpf1 variant that recognizes TYCV PAMs

Depositor

Feng Zhang

Article

Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

Insert

Cpf1, RR variant

Use

CRISPR

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

S542R/K607R

Promoter

CMV

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pR26 CAG/BFP Dest

Plasmid

#74282

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, BFP, for gateway cloning

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Available Since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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pGEL1029

Plasmid

#225600

Purpose

The STU backbone vector for tomato genome editing is based on Mb3Cas12a-RRR (D172R/N573R/K579R)

Depositor

Yong Zhang

Article

Liu et al Plant Biotechnol J. 2024 Oct 10. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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AA012

Plasmid

#215974

Purpose

Fragmid fragment: (N' terminus) Bipartite Nuclear Localization Signal

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; BPNLS_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBK-VRER-BE3

Plasmid

#85173

Purpose

Expresses VRER-BE3 in mammalian cells

Depositor

David Liu

Article

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

VRER-BE3

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pAcHELIX-sgRNA_entry (CJT94)

Plasmid

#181783

Purpose

Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k

Expression

Bacterial

Mutation

nAniI = K227M, F80K, L232K

Promoter

Lac and J23119

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pGWB442

Plasmid

#74828

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with eYFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pVenus-VSVG

Plasmid

#11914

Depositor

Jennifer Lippincott-Schwartz

Article

Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

Insert

VSVG

Expression

Mammalian

Mutation

F46L, F64L, M153T, V163A, S175G, T203Y, A206K rel…

Available Since

Sept. 13, 2006

Availability

Academic Institutions and Nonprofits only

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AAV-hRedOpsin-TGFB1

Plasmid

#164427

Purpose

AAV plasmid expressing TGFB1 in photoreceptors

Depositor

Connie Cepko

Article

Wang et al J Clin Invest. 2020 Aug 3;130(8):4360-43

Insert

Tgfb1 (Tgfb1 Mouse)

Use

AAV

Expression

Mammalian

Promoter

human red opsin

Available Since

Nov. 20, 2021

Availability

Academic Institutions and Nonprofits only

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