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pLX-EF1a_Empty

Plasmid

#221480

Purpose

Empty vector control for pLX307 overexpresion experiments

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_PsCatCh2.0-YFP

Plasmid

#186978

Purpose

Expresses PsCatCh2.0 channelrhodopsin in mammalian cells

Depositor

Adam Cohen

Article

Hayward et al eLife. 2023 Oct 6;12:RP86833. doi: 10.75

Insert

PsCatCh2.0-YFP

Use

Lentiviral

Tags

eYFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGE_En01

Plasmid

#71534

Purpose

Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

Depositor

Ikuko Hara-Nishimura, Takayuki Kohchi

Article

Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pBSM

Plasmid

#67505

Purpose

Express the tRNAMet scaffold

Depositor

Luc Ponchon

Article

Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

Insert

modified tRNAMet

Expression

Bacterial

Promoter

lpp

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
attB-GFP

Plasmid

#65521

Purpose

Plasmid for the generation of GFP-knockin fusions via Bxb1-mediated recombination into MIN-tagged cell lines

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-GFP

Use

Mouse Targeting; Bxb1

Expression

Mammalian

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-FLPX-rc [Jaws-KGC-tdTomato-ER2]

Plasmid

#195488

Purpose

AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CAG promoter, in FLPX/reversed (Frt-dependent) manner.

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-KGC-tdTomato-ER2

Use

AAV

Tags

ER2, KGC, and tdTomato

Expression

Mammalian

Mutation

K200R W214F

Promoter

CAG

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCFJ2475 - pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

Plasmid

#159813

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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1068 pGL3 FasL promoter (no SV40 prom)

Plasmid

#9029

Depositor

William Sellers

Article

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

Fas ligand promoter (FASLG Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Removed the SV40 promoter from pGL3

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pAAV-GFAP-eLACCO1.1

Plasmid

#168790

Purpose

Astrocyte expression of fluorescent sensor for extracellular lactate

Depositor

Marie-Eve Paquet

Article

Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

Insert

eLacco1.1

Use

AAV

Tags

ER export and GPI

Expression

Mammalian

Promoter

GFAP

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD-eLACCO1.1

Plasmid

#167944

Purpose

Fluorescent biosensor for extracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

Insert

eLACCO1.1

Expression

Bacterial

Promoter

araBAD

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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pRSF-FGE

Plasmid

#102615

Purpose

Inducible expression of Formylglycine-generating enzyme

Depositor

Marc Wold

Article

Bain et al Methods Enzymol. 2018;600:439-461. doi:

Insert

Formylglycine-generating enzyme

Expression

Bacterial

Promoter

T7

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCri-13a

Plasmid

#61321

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag and MISTIC-tag

Expression

Bacterial

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

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deltaMyc-HA

Plasmid

#16556

Depositor

Bert Vogelstein

Article

Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

Insert

c-MYC (MYC Human)

Tags

HA

Expression

Mammalian

Mutation

Deletion of aa 40-178

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
pAAV-Ef1a-DIO mScarlet (AAV5)

Viral Prep

#131002-AAV5

Purpose

Ready-to-use AAV5 particles produced from pAAV-Ef1a-DIO mScarlet (#131002). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO mScarlet plasmid DNA. EF1a-driven, Cre-dependent mScarlet expression. These AAV preparations are suitable purity for injection into animals.

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Promoter

EF1a

Tags

mScarlet (Cre-dependent)

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only
pUBC-SV40NLS-Pfv-Sapphire

Plasmid

#203653

Purpose

Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter.

Depositor

Liam Holt

Article

Shu T et et al bioRxiv 2022

Insert

PfV

Use

Lentiviral

Tags

Sapphire

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-UGAcS

Plasmid

#172135

Purpose

mammalian expression plasmid for a UDP-GlcNAc sensor

Depositor

Huiwang Ai

Article

Li et al ACS Cent Sci. 2021 Oct 27;7(10):1763-177

Insert

UGAcS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pBSV2B

Plasmid

#118228

Purpose

Empty E. coli - B. burgdorferi shuttle vector, blasticidin S resistant

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pET14-Encap

Plasmid

#86405

Purpose

Expresses T. maritima encapsulin in E. coli

Depositor

David Savage

Article

Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

Insert

Encapsulin

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pME-GFP-P2A

Plasmid

#80807

Purpose

GFP without stop codon followed by porcine teschovirus-1 2A (P2A) "self-cleaving" peptide

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

GFP-P2A

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pBSMrnaSeph

Plasmid

#67507

Purpose

Express the tRNAMet scaffold with the sephadex aptamer tag

Depositor

Luc Ponchon

Article

Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

Insert

modified tRNAMet with sephadex aptamer

Expression

Bacterial

Promoter

lpp

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only