We narrowed to 6,005 results for: 338

1. Lrrc4-Fc-His

   Plasmid

   #72084

   Purpose

   Expresses the extracellular region of the LRRC4 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Lrrc4 (Lrrc4 Mouse)
   

   Use

   Tags

   Fc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-SFFV-FLAG-TurboID-dPB1-SQSTM1

   Plasmid

   #223716

   Purpose

   Expresses FLAG-TurboID-dPB1-SQSTM1

   Depositor

   Benjamin Wolozin

   Article

   Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

   Insert

   SQSTM1 (SQSTM1 Human)
   

   Use

   Lentiviral

   Tags

   TurboID

   Expression

   Mammalian

   Mutation

   deletion 1-119

   Promoter

   SFFV

   Available since

   Aug. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pHR-SFFV-FLAG-TurboID-dUBA-SQSTM1

   Plasmid

   #223717

   Purpose

   Expresses FLAG-TurboID-dUBA-SQSTM1

   Depositor

   Benjamin Wolozin

   Article

   Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

   Insert

   SQSTM1 (SQSTM1 Human)
   

   Use

   Lentiviral

   Tags

   TurboID

   Expression

   Mammalian

   Mutation

   deletion 384-Stop codon

   Promoter

   SFFV

   Available since

   Aug. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pDN-D2irC6kwh

   Plasmid

   #44725

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

   Inserts

   pCMV-D2i promoter
   mCherry
   

   Use

   Synthetic Biology; Expression regulator/reporter

   Tags

   Rabbit β-globin intron II and WPRE

   Expression

   Mammalian

   Mutation

   Initiator motif (Inr) displaced relative to pCMV-…

   Promoter

   pCMV-D2i

   Available since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGex-4T-2_GEC1

   Plasmid

   #73945

   Purpose

   For recombinant expression of human GEC1/GABARAPL1 in E. coli

   Depositor

   Dieter Willbold

   Article

   Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

   Insert

   GEC1 (GABARAPL1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

6. TFORF2483

   Plasmid

   #142104

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CBX2 (CBX2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21a-Med25-VBD-6His

   Plasmid

   #64773

   Purpose

   Bacterial expression of His-tagged Med25-VBD

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

   Insert

   Med25-VBD (MED25 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   insert is 394-543 only

   Promoter

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

8. EPHB1_HUMAN_D0

   Plasmid

   #79694

   Purpose

   This plasmid encodes the kinase domain of EPHB1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, Markus Seeliger, John Chodera

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   EPHB1 (EPHB1 Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

9. TFORF1493

   Plasmid

   #143286

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   FOSL1 (FOSL1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. C2-77 pComb3xss anti-cFos nanobody

    Plasmid

    #145837

    Purpose

    Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-cFos (Human) recombinant llama nanobody C2-77 (FOS L. glama (llama))
    

    Use

    Tags

    6xHis-HA

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. GC83 pComb3xss anti-Gephyrin nanobody

    Plasmid

    #134721

    Purpose

    Bacterial expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an immunolabel

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-Gephyrin (Human) recombinant llama nanobody GC83 (GPHN L. glama (llama))
    

    Use

    Tags

    6xHis-HA

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. STK3_HUMAN_D0

    Plasmid

    #79689

    Purpose

    This plasmid encodes the kinase domain of STK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, Markus Seeliger, John Chodera

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    STK3 (STK3 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pENTR221 UBR5 ∆PABC

    Plasmid

    #81064

    Purpose

    Gateway entry vector encoding full length UBR5 Y2402A/K2415A (∆PABC)

    Depositor

    Darren Saunders

    Article

    Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

    Insert

    Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆PABC (UBR5 Human)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Y2402A and K2415A (bp t7204g, a7205c, t7206c, a72…

    Promoter

    N/A

    Available since

    Jan. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH-puro/Flag PD-L1 4NQ

    Plasmid

    #121456

    Purpose

    expression of human PD-L1 mutant in mammalian cells

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    CD274 (CD274 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Dscaml1-Fc-His

    Plasmid

    #72072

    Purpose

    Expresses the extracellular region of the Dscaml1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Dscaml1 (Dscaml1 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL3 3'UTR reporter MUT 1.3 kb CD274 Hs 3'UTR Final

    Plasmid

    #107010

    Purpose

    Modified Luciferase expression vector

    Depositor

    Julian Downward

    Article

    Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

    Insert

    CD274 (CD274 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. EPHB1-bio-His

    Plasmid

    #51750

    Purpose

    Expresses full-length Ephrin type-B receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    EPHB1 (EPHB1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSLIK-NFLAG-hFUS

    Plasmid

    #50487

    Purpose

    Produces lentivirus expressing human FUS with FLAG tag at its N-terminus by co-transfection with psPAX2 and pMD2G

    Depositor

    Zissimos Mourelatos

    Article

    Nakaya et al RNA. 2013 Apr;19(4):498-509. doi: 10.126

    Insert

    FUS (FUS Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

19. S1-71 pComb3xss anti-cFos nanobody

    Plasmid

    #145832

    Purpose

    Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-cFos (Human) recombinant llama nanobody S1-71 (FOS L. glama (llama))
    

    Use

    Tags

    6xHis-HA

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pv6_MECP2_MBD

    Plasmid

    #179407

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    MECP2_MBD (Mecp2 Mouse)
    

    Use

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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