We narrowed to 30,517 results for: Ide

1. pCW57.1-DUX4-WT

   Plasmid

   #99282

   Purpose

   All-in-one lentivector with tet-inducible DUX4 (wildetype nucleotide sequence)

   Depositor

   Stephen Tapscott

   Article

   Jagannathan et al Hum Mol Genet. 2016 Oct 15;25(20):4419-4

   Insert

   DUX4 (DUX4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pY027

   Plasmid

   #84742

   Purpose

   Expresses huLbCpf1 and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Insert

   huLbCpf1
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmCherry_sgRNA-ver2

   Plasmid

   #126776

   Purpose

   gRNA expression vector with mCherry transfection control, does not contain the direct repeat of the truncated guideRNA

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   sgRNA (for SpCas9)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAVS1-TRE-SHH

   Plasmid

   #134350

   Purpose

   Targeting vector for site specific integration of doxycycline inducible expression of full length human SHH cDNA into human AAVS1 locus

   Depositor

   Lorenz Studer

   Article

   Cederquist et al Nat Biotechnol. 2019 Apr;37(4):436-444.

   Insert

   SHH (SHH Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2-UTX-F

   Plasmid

   #17438

   Purpose

   Expression of UTX in mammalian cells

   Depositor

   Kai Ge

   Article

   Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

   Insert

   UTX (KDM6A Human)
   

   Use

   Tags

   6xMyc and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. hGip-R

   Plasmid

   #14942

   Depositor

   Bernard Thorens

   Article

   Gremlich et al Diabetes. 1995 Oct . 44(10):1202-8.

   Insert

   Gip-R (GIPR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Short form of the receptor

   Promoter

   Available since

   June 7, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pUASp-GBP-Tbro

   Plasmid

   #179995

   Purpose

   Expresses two tandem copies of the GFP nanobody fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system. The UASp version is ideal for expression in the germline.

   Depositor

   Graydon Gonsalvez

   Article

   Baker et al Development. 2021 Nov 15;148(22). pii: 2

   Insert

   GBP nanobody
   

   Use

   Tags

   Trbo

   Expression

   Insect

   Mutation

   Promoter

   UAS-Gal4

   Available since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-mFlagEts1

   Plasmid

   #86099

   Purpose

   expresses flag tagged Ets1 in mammalian cells

   Depositor

   Barbara Graves

   Article

   Seidel et al Genes Dev. 2002 Jan 1;16(1):127-37.

   Insert

   Ets1 (Ets1 Mouse)
   

   Use

   Tags

   Flag tag; kanamycin selection

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Burden Monitor lambda version

   Plasmid

   #66073

   Purpose

   The burden monitor was cloned inside plasmid pAH63 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cells

   Depositor

   Tom Ellis

   Article

   Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

   Insert

   GFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pEJS2437_Nme2.Smu-ABE8e-nt

    Plasmid

    #201519

    Purpose

    Mammalian expression of N-terminally fused Nme2.Smu-ABE8e-nt

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2.Smu-ABE8e-nt
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNW-sfGFP(SPS6)

    Plasmid

    #133814

    Purpose

    Fluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides.

    Depositor

    Oscar Kuipers

    Article

    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

    Insert

    sfGFP(SPS6)
    

    Use

    E.coli/bacillus spp. shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ppta

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLHCX-gpASNase1

    Plasmid

    #121526

    Purpose

    Expresses gpASNase1 in mammalian cells.

    Depositor

    Lucas Sullivan, Matthew Vander Heiden

    Article

    Sullivan et al Nat Cell Biol. 2018 Jul;20(7):782-788. d

    Insert

    gpASNase1
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. hHSF1 K80R

    Plasmid

    #118350

    Purpose

    Plasmid expresses hHSF1 with K80 in DBD mutated to R.

    Depositor

    Lea Sistonen

    Article

    Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

    Insert

    hHSF1 (HSF1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    K80R

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL3_5UTR_Myc_FLuc

    Plasmid

    #229502

    Purpose

    Myc 5' untranslated region firefly luciferase reporter.

    Depositor

    Davide Ruggero

    Article

    Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

    Insert

    Myc 5'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. Brp-T2A-QF2w-Hsp70-3XP3-dsRed

    Plasmid

    #141094

    Purpose

    Template plasmid for inserting QF2w into the Brp gene of Aedes aegypti with CRISPR/Cas9, to generate a pan-neuronal driver line.

    Depositor

    Carolyn McBride

    Article

    Zhao et al Cell Rep Methods. 2021 Jul 26;1(3):10004

    Insert

    bruchpilot
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    coding sequence downstream of CRISPR cut site, sy…

    Promoter

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC3.1_CAG_oROS-Gr

    Plasmid

    #216113

    Purpose

    Expresses the genetically encoded, ratiometric hydrogen peroxide sensor oROS-Gr in mamalian cells.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.01.31.578117

    Insert

    CAG_oROS-Gr
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Grp1-PH pGEX2TK

    Plasmid

    #71379

    Purpose

    bacterial expression of PH domain for phosphoinositide binding assay

    Depositor

    Mark Lemmon

    Article

    Kavran et al J Biol Chem. 1998 Nov 13;273(46):30497-5

    Insert

    Grp1-PH (Cyth3 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Pleckstrin homology (PH) domain; aa 264-391

    Promoter

    tac

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pDUAL_tacI

    Plasmid

    #155170

    Purpose

    BaSP and CuCBP co-expression: tacI promoter controls BaSP expression

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDT-sgRNA-XMAS-1x

    Plasmid

    #164413

    Purpose

    Dual targeted guide and cloning vector. Targets pEF-XMAS-1xStop. Guides targeting endogenous sites can be cloned into BbsI sites.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDUAL_2_araBAD

    Plasmid

    #155176

    Purpose

    see pDUAL_araBAD: exchanged origin of replication (pBR322) and introduced bom site

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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