We narrowed to 9,378 results for: mel

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pFLAG AXL

Plasmid

#105933

Purpose

Mammalian expression of Flag-tagged AXL

Depositor

Rosa Marina Melillo

Article

Krishnamoorthy et al J Biol Chem. 2013 Jun 14;288(24):17481-9

Insert

AXL (AXL Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERH-ZNF281

Plasmid

#222923

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of ZNF281

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

ZNF281 (ZNF281 Human)

Expression

Mammalian

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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4xnrUAS:MLS-KillerRed in pTol2pA2-acrys-EGFP-

Plasmid

#115516

Purpose

Plasmid driving expression of membrane bound KillerRed fluorophore under UAS response element, with a green lens reporter

Depositor

Marco Morsch

Article

Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

Insert

MLS-KillerRed

Use

Gateway Cloning

Tags

MLS

Promoter

UAS

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pET28 MBP NAAEF-AMPKa1 (13-476,529-550)-b2(76-272)-g1(24-327)

Plasmid

#177850

Purpose

Bacterial Expression of AMPK

Depositor

Karsten Melcher

Article

Yan et al Science. 2021 Jul 23;373(6553):413-419.

Insert

AMPK (a1b2g1)

Tags

MBP

Expression

Bacterial

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1a-2xSV40_NLS-NLuc

Plasmid

#135953

Purpose

Expresses the NanoLuciferase targeted to the nucleus in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

NanoLuciferase

Tags

2xSV40-NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pGATOR-DTA

Plasmid

#64103

Purpose

Tet inducible, Gateway adapted ROSA26 targeting Vector with DTA

Depositor

Bill Skarnes

Article

Melidoni et al Proc Natl Acad Sci U S A. 2013 Oct 29;11

Insert

rTTA (Gt(ROSA)26Sor Synthetic)

Use

Mouse Targeting; Tet inducible

Expression

Mammalian

Promoter

ROSA26 endogenous

Available Since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

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HA-USPL1-pcDNA3.1

Plasmid

#85760

Purpose

mammalian expression of full length HA tagged USPL1

Depositor

Frauke Melchior

Article

Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

Insert

USPL1, full length (USPL1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CBA-DIO-GCaMP6s-P2A-mBeRFP-WPRE-pA

Plasmid

#175177

Purpose

Cre-dependent GCaMP6s and mBeRFP expression

Depositor

Bernardo Sabatini

Article

Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

Insert

GCaMP6s

Use

AAV

Tags

P2A-mBeRFP

Promoter

CAG

Available Since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAd MafA-I-nGFP

Plasmid

#19412

Depositor

Douglas Melton

Article

Zhou et al Nature. 2008 Oct 2;455(7213):627-32. doi

Insert

v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian) (Mafa Mouse)

Use

Adenoviral and Gateway Cloning

Tags

GFP and IRES

Available Since

Nov. 12, 2008

Availability

Academic Institutions and Nonprofits only
pET28C-mCherry-DDX4LCD

Plasmid

#204408

Purpose

E. coli expression of DDX4 low-complexity domain (1-236) with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

DDX4(1-236) (DDX4 Human)

Tags

6x-His; mCherry

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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MiniCoopR U6:gRNA spred1, mitfa:Cas9

Plasmid

#118843

Purpose

Targets spred1 and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

spred1 gRNA (spred1 Zebrafish)

Use

CRISPR; Tol2

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pET28C-mCherry-53BP1LCD

Plasmid

#204407

Purpose

E. coli expression of the 53BP1 low-complexity domain (1208-1977) with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

53BP1(1208-1977) (TP53BP1 Human)

Tags

6x-His; mCherry

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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"AND-gate" receiver plasmid

Plasmid

#127676

Purpose

Encodes the transcriptional activator LuxR and GFP. LuxR has an pLlacO-1 promoter and the GFP has a pLux promoter.

Depositor

Friedrich Simmel

Article

Schwarz-Schilling et al Integr Biol (Camb). 2016 Apr 18;8(4):564

Insert

LuxR, GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1 for LuxR, pLux for GFP

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pET23a-His-hRanGAP1tail

Plasmid

#53139

Purpose

E.coli expression of Cterminal domain of human RanGAP1 with Nterminal His-tag

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

RanGAP1 (RANGAP1 Human)

Tags

His

Expression

Bacterial

Mutation

C-terminal fragment (amino acids 398-587)

Promoter

T7

Available Since

May 30, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV CAG-DO-TC66T-mCherry

Plasmid

#175178

Purpose

Cre-off TVA-mCherry fusion protein with 66T mutation

Depositor

Bernardo Sabatini

Article

Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

Insert

TVA

Use

AAV

Tags

mCherry

Mutation

E66T

Promoter

CAG

Available Since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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dmBACCS2VL-IRES-dOrai

Plasmid

#67629

Purpose

Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, and dOrai

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2VL-IRES-dOrai

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pEF1a-2xSV40_NLS-CeNLuc

Plasmid

#135952

Purpose

Expresses the CeNLuc LumiFluor targeted to the nucleus in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

CeNLuc LumiFluor

Tags

2xSV40-NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pEF1a-T2-CeNLuc

Plasmid

#135948

Purpose

Expresses the CeNLuc LumiFluor targeted to the mitochondira in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

CeNLuc LumiFluor

Tags

T2 mitochondrial localization sequence

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-double floxed-Aldh1a1-HA-WPRE-HGHpA

Plasmid

#184633

Purpose

Expresses Aldh1a1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

Depositor

Nicolas Tritsch

Article

Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

Insert

Aldh1a1 (Aldh1a1 Mouse)

Use

AAV

Promoter

EF1a

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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1098A=TI-pgSIT[sxl,bTub,Hasp70Bb-Cas9]

Plasmid

#149426

Purpose

The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel sxl and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

Inserts

U6.3-gRNAs[Sxl, bTub]
Hsp70Bb-Cas9-T2A-eGFP-p10

Use

CRISPR

Tags

eGFP

Expression

Insect

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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